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2.4.1.170: isoflavone 7-O-glucosyltransferase

This is an abbreviated version!
For detailed information about isoflavone 7-O-glucosyltransferase, go to the full flat file.

Word Map on EC 2.4.1.170

Reaction

UDP-glucose
+
an isoflavone
=
UDP
+
an isoflavone 7-O-beta-D-glucoside

Synonyms

AtGT-2, F7GT, glucosyltransferase, uridine diphosphoglucose-isoflavone 7-O-, GmIF7GT, GmIF7GT1, GmUGT1, GmUGT3, GmUGT4, GmUGT7, GmUGT9, IF7GT, IFGT, isoflavone-7-O-glucosyltransferase, isoflavone-specific 7-O-glucosyltransferase, More, PlUGT1, UDP-glucose-flavonoid 7-O-glucosyltransferase, UDP-glucose: formononetin 7-O-glucosyltransferase, UDP-glucose:isoflavone 7-O-glucosyltransferase, UDPglucose-flavonoid 7-O-glucosyltransferase, UDPglucose:isoflavone 7-O-glucosyltransferase, UGT4, UGT709A4, UGT73F1, uridine diphosphoglucose-isoflavone 7-O-glucosyltransferase

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.170 isoflavone 7-O-glucosyltransferase

Purification

Purification on EC 2.4.1.170 - isoflavone 7-O-glucosyltransferase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
DEAE-Sepharose Fast Flow column, Q-Sepharose HP column, Phenyl-Sepharose HP column (pH 8.5), hydroxyapatite column, gel filtration (HiLoad 26/60 Superdex 200pg column), resource Q column and chromatofocusing on Mono P (Mono P column), all at 4°C. The activity in the crude extract is extremely unstable to oxidation and the addition of 2-mercaptoethanol is essential for its efficient purification. The addition of CHAPS to purification buffers is also essential for purification after step 6 (hydroxyapatite chromatography), otherwise the activity is irreversibly lost.
-
recombinant enzyme from Escherichia coli
-
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) to near homogeneity by nickel affinity chromatography
-