2.4.1.170: isoflavone 7-O-glucosyltransferase
This is an abbreviated version!
For detailed information about isoflavone 7-O-glucosyltransferase, go to the full flat file.
Word Map on EC 2.4.1.170
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2.4.1.170
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soybean
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isoflavonoids
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cotyledon
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daidzin
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kaempferol
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genistin
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2-hydroxyisoflavanone
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aglycones
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echinata
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glycyrrhiza
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ammonia-lyase
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phytoalexins
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long-distance
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luteolin
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flooding
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bradyrhizobium
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sojae
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pueraria
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cell-suspension
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phytophthora
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plant-microbe
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lobata
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enrei
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naringenin
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glycitin
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cicer
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glucosyltransferases
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arietinum
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drug development
- 2.4.1.170
- soybean
-
isoflavonoids
- cotyledon
- daidzin
- kaempferol
- genistin
- 2-hydroxyisoflavanone
-
aglycones
- echinata
-
glycyrrhiza
-
ammonia-lyase
-
phytoalexins
-
long-distance
- luteolin
-
flooding
-
bradyrhizobium
- sojae
-
pueraria
-
cell-suspension
-
phytophthora
-
plant-microbe
- lobata
-
enrei
- naringenin
- glycitin
-
cicer
-
glucosyltransferases
- arietinum
- drug development
Reaction
Synonyms
AtGT-2, F7GT, glucosyltransferase, uridine diphosphoglucose-isoflavone 7-O-, GmIF7GT, GmIF7GT1, GmUGT1, GmUGT3, GmUGT4, GmUGT7, GmUGT9, IF7GT, IFGT, isoflavone-7-O-glucosyltransferase, isoflavone-specific 7-O-glucosyltransferase, More, PlUGT1, UDP-glucose-flavonoid 7-O-glucosyltransferase, UDP-glucose: formononetin 7-O-glucosyltransferase, UDP-glucose:isoflavone 7-O-glucosyltransferase, UDPglucose-flavonoid 7-O-glucosyltransferase, UDPglucose:isoflavone 7-O-glucosyltransferase, UGT4, UGT709A4, UGT73F1, uridine diphosphoglucose-isoflavone 7-O-glucosyltransferase
ECTree
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Purification
Purification on EC 2.4.1.170 - isoflavone 7-O-glucosyltransferase
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DEAE-Sepharose Fast Flow column, Q-Sepharose HP column, Phenyl-Sepharose HP column (pH 8.5), hydroxyapatite column, gel filtration (HiLoad 26/60 Superdex 200pg column), resource Q column and chromatofocusing on Mono P (Mono P column), all at 4°C. The activity in the crude extract is extremely unstable to oxidation and the addition of 2-mercaptoethanol is essential for its efficient purification. The addition of CHAPS to purification buffers is also essential for purification after step 6 (hydroxyapatite chromatography), otherwise the activity is irreversibly lost.
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recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) to near homogeneity by nickel affinity chromatography
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