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(22S,25S)-5alpha-solanidanin-3beta-ol + UDP-glucose
?
-
demissidine 1.4% of the activity with sitosterol (stigmast-5-en-3beta-ol)
-
-
?
(22S,25S)-epoxy-furost-5-en-3beta,26-diol + UDP-glucose
?
-
nuatigenin, 16.1% of the activity with sitosterol (stigmast-5-en-3beta-ol)
-
-
?
(25R)-5alpha-spirostan-3beta-ol + UDP-glucose
?
-
tigogenin, 2.1% of the activity with sitosterol (stigmast-5-en-3beta-ol)
-
-
?
(25R)-5alpha-spirostan-3beta-ol-12-one + UDP-glucose
?
-
hecogenin, 6.3% of the activity with sitosterol (stigmast-5-en-3beta-ol)
-
-
?
(25R)-spirost-5-en-3beta,25beta-diol + UDP-glucose
?
-
isonuatigenin, 13.4% of the activity with sitosterol (stigmast-5-en-3beta-ol)
-
-
?
(25R)-spirost-5-en-3beta-ol + UDP-glucose
?
-
diosgenin, 3.5% of the activity with sitosterol (stigmast-5-en-3beta-ol)
-
-
?
(25S)-5alpha,22betaN-spirosolanin-3beta-ol + UDP-glucose
?
-
tomatidine, 2.8% of the activity with sitosterol (stigmast-5-en-3beta-ol)
-
-
?
24-methylene cholesterol + UDP-glucose
UDP + 24-methylene-cholesterol 3-beta-D-glucoside
-
-
-
-
?
3beta-hydroxy-16,17alpha-epoxypregnenolone + UDP-alpha-D-glucose
3beta-hydroxy-16,17alpha-epoxypregnenolone 3-beta-D-glucoside + UDP
-
-
-
-
?
3beta-hydroxy-pregna-5,16-dien-20-one + UDP-glucose
UDP + 3beta-20-oxopregna-5,16-dien-3-yl beta-D-glucopyranoside
5alpha-cholest-7-en-3beta-ol + UDP-glucose
?
-
14.1% of the activity with sitosterol (stigmast-5-en-3beta-ol)
-
-
?
5alpha-cholestan-3beta-ol + UDP-glucose
?
-
cholestanol, 12.0% of the activity with sitosterol (stigmast-5-en-3beta-ol)
-
-
?
5alpha-cholestan-3beta-ol + UDP-glucose
UDP + 3beta,5alpha-cholestan 3-beta-D-glucoside
-
-
-
-
?
androst-5-en-3beta-ol-17-one + UDP-glucose
?
-
androstenolon, 16.0% of the activity with sitosterol (stigmast-5-en-3beta-ol)
-
-
?
beta-sitosterol + UDP-glucose
UDP + beta-sitosterol 3-beta-D-glucoside
brassicasterol + UDP-glucose
UDP + brassicasterol 3-beta-D-glucoside
-
-
-
-
?
cholest-5-en-22-on-3beta-ol + UDP-glucose
?
-
22-oxycholesterol, 133.1% of the activity with sitosterol (stigmast-5-en-3beta-ol)
-
-
?
cholest-5-en-3beta,19-diol + UDP-glucose
?
-
9.2% of the activity with sitosterol (stigmast-5-en-3beta-ol)
-
-
?
cholest-5-en-3beta,20alpha-diol + UDP-glucose
?
-
90.8% of the activity with sitosterol (stigmast-5-en-3beta-ol)
-
-
?
cholest-5-en-3beta,25-diol + UDP-glucose
?
-
25-hydroxycholesterol, 89.4% of the activity with sitosterol (stigmast-5-en-3beta-ol)
-
-
?
cholest-5-en-3beta-ol + UDP-glucose
?
-
cholesterol, 98.5% of the activity with sitosterol (stigmast-5-en-3beta-ol)
-
-
?
cholest-5-en-3beta-ol-7-one + UDP-glucose
?
-
5.6% of the activity with sitosterol (stigmast-5-en-3beta-ol)
-
-
?
cholesterol + UDP-glucose
UDP + cholesterol 3-beta-D-glucoside
dehydroepiandrosterone + UDP-glucose
UDP + dehydroepiandrosterone 3-beta-D-glucoside
diosgenin + UDP-glucose
UDP + diosgenin 3-beta-D-glucoside
-
-
-
-
?
ergosterol + UDP-glucose
UDP + ergosterol 3-beta-D-glucoside
-
-
-
-
?
pregn-5-en-3beta-ol-20-one + UDP-glucose
?
-
pregnenolon, 33.1% of the activity with sitosterol (stigmast-5-en-3beta-ol)
-
-
?
pregnenolene + UDP-glucose
UDP + pregnenolone 3-beta-D-glucoside
-
-
-
-
?
pregnenolone + UDP-glucose
UDP + pregnenolone 3-beta-D-glucoside
-
-
-
-
?
solasodine + UDP-glucose
UDP + solasodine 3-beta-D-glucoside
-
-
-
-
?
stigmasta-5,22-dien-3beta-ol + UDP-glucose
?
-
stigmasterol, 49.2% of the activity with sitosterol (stigmast-5-en-3beta-ol)
-
-
?
stigmasta-5,24(28)-dien-3beta-ol + UDP-glucose
?
-
82.4% of the activity with sitosterol (stigmast-5-en-3beta-ol)
-
-
?
stigmastan-3beta-ol + UDP-glucose
?
-
38.7% of the activity with sitosterol (stigmast-5-en-3beta-ol)
-
-
?
stigmasterol + UDP-glucose
UDP + stigmasterol 3-beta-D-glucoside
UDP-alpha-D-glucose + testosterone
UDP + testosterone 17-beta-D-glucoside
UDP-glucose + 3beta-hydroxy-16,17alpha-epoxypregnenolone
?
-
-
-
?
UDP-glucose + 3beta-hydroxy-16,17alpha-epoxypregnenolone
UDP + 3beta-hydroxy-16,17alpha-epoxypregnenolone 3-beta-D-glucoside
-
-
-
-
?
UDP-glucose + 3beta-hydroxy-pregna-5,16-dien-one
UDP + 3beta-20-oxopregna-5,16-dien-3-yl beta-D-glucopyranoside
-
-
-
?
UDP-glucose + 4-demethyl-obtusifoliol
UDP + 4-demethyl-obtusifoliol 3-beta-glucoside
-
58% of the activity with sitosterol
-
?
UDP-glucose + 5-alpha-cholestanol
UDP + 5-alpha-cholestanol 3-beta-D-glucoside
-
8% of the activity with sitosterol
-
?
UDP-glucose + 5-cholestenol
UDP + 5-cholestenol 3-beta-D-glucoside
-
12% of the activity with sitosterol
-
?
UDP-glucose + 7-dehydrocholesterol
UDP + 7-dehydrocholesterol 3-beta-D-glucoside
-
6% of the activity with sitosterol
-
?
UDP-glucose + a sterol
UDP + a sterol 3-beta-D-glucoside
UDP-glucose + alpha-spinasterol
UDP + alpha-spinasterol 3-beta-D-glucoside
-
31% of the activity with sitosterol
-
?
UDP-glucose + beta-sitosterol
UDP + beta-sitosterol 3-beta-D-glucoside
UDP-glucose + brassicasterol
UDP + brassicasterol 3-beta-D-glucoside
UDP-glucose + campesterol
UDP + campesterol 3-beta-D-glucoside
UDP-glucose + cholesterol
UDP + cholesterol 3-beta-D-glucoside
UDP-glucose + dehydroepiandrosterone
UDP + dehydroepiandrosterone 3-beta-D-glucoside
-
-
-
?
UDP-glucose + epiandrosterone
UDP + epiandrosterone 3-beta-glucoside
-
best substrate
-
?
UDP-glucose + ergosterol
UDP + ergosterol 3-beta-D-glucoside
UDP-glucose + estradiol
UDP + estradiol 3-beta-D-glucoside
UDP-glucose + estrone
UDP + estrone 3-beta-D-glucoside
UDP-glucose + isofucosterol
UDP + isofucosterol 3-beta-glucoside
-
73% of the activity with sitosterol
-
?
UDP-glucose + poriferasterol
UDP + poriferasterol 3-beta-glucoside
-
synthesis of poriferasterol monoglucoside may be involved in differentiation
-
-
?
UDP-glucose + pregnenolone
UDP + pregnenolone 3-beta-D-glucoside
UDP-glucose + pregnenolone
UDP + pregnenolone 3-beta-glucoside
-
57% of the activity with epiandrosterone
-
?
UDP-glucose + protopanaxadiol
UDP + protopanaxadiol 3-beta-D-glucoside
UDP-glucose + sitosterol
UDP + sitosterol 3-beta-D-glucoside
UDP-glucose + solasodine
UDP + solasodine 3-beta-D-glucoside
-
-
-
?
UDP-glucose + stigmasterol
UDP + stigmasterol 3-beta-D-glucoside
UDP-glucose + tomatidine
UDP + tomatidine 3-beta-glucoside
UDP-glucose + transandrosterone
UDP + transandrosterone 3beta-D-glucoside
UDP-glucose + withaferin A
UDP + ?
-
-
-
?
UDP-glucose + withanolide A
UDP + ?
-
-
-
?
additional information
?
-
3beta-hydroxy-pregna-5,16-dien-20-one + UDP-glucose
UDP + 3beta-20-oxopregna-5,16-dien-3-yl beta-D-glucopyranoside
-
-
-
-
?
3beta-hydroxy-pregna-5,16-dien-20-one + UDP-glucose
UDP + 3beta-20-oxopregna-5,16-dien-3-yl beta-D-glucopyranoside
-
-
-
-
?
beta-sitosterol + UDP-glucose
UDP + beta-sitosterol 3-beta-D-glucoside
-
-
-
-
?
beta-sitosterol + UDP-glucose
UDP + beta-sitosterol 3-beta-D-glucoside
-
-
-
-
?
cholesterol + UDP-glucose
UDP + cholesterol 3-beta-D-glucoside
-
-
-
-
?
cholesterol + UDP-glucose
UDP + cholesterol 3-beta-D-glucoside
-
-
-
-
?
dehydroepiandrosterone + UDP-glucose
UDP + dehydroepiandrosterone 3-beta-D-glucoside
-
-
-
-
?
dehydroepiandrosterone + UDP-glucose
UDP + dehydroepiandrosterone 3-beta-D-glucoside
-
-
-
-
?
stigmasterol + UDP-glucose
UDP + stigmasterol 3-beta-D-glucoside
-
-
-
-
?
stigmasterol + UDP-glucose
UDP + stigmasterol 3-beta-D-glucoside
-
-
-
-
?
UDP-alpha-D-glucose + testosterone
UDP + testosterone 17-beta-D-glucoside
-
-
-
?
UDP-alpha-D-glucose + testosterone
UDP + testosterone 17-beta-D-glucoside
-
-
-
?
UDP-alpha-D-glucose + testosterone
UDP + testosterone 17-beta-D-glucoside
-
-
-
?
UDP-alpha-D-glucose + testosterone
UDP + testosterone 17-beta-D-glucoside
-
-
-
?
UDP-alpha-D-glucose + testosterone
UDP + testosterone 17-beta-D-glucoside
-
-
-
?
UDP-alpha-D-glucose + testosterone
UDP + testosterone 17-beta-D-glucoside
-
-
-
?
UDP-alpha-D-glucose + testosterone
UDP + testosterone 17-beta-D-glucoside
-
-
-
?
UDP-alpha-D-glucose + testosterone
UDP + testosterone 17-beta-D-glucoside
-
-
-
?
UDP-alpha-D-glucose + testosterone
UDP + testosterone 17-beta-D-glucoside
-
-
-
?
UDP-alpha-D-glucose + testosterone
UDP + testosterone 17-beta-D-glucoside
-
-
-
?
UDP-alpha-D-glucose + testosterone
UDP + testosterone 17-beta-D-glucoside
-
-
-
?
UDP-alpha-D-glucose + testosterone
UDP + testosterone 17-beta-D-glucoside
-
-
-
?
UDP-glucose + a sterol
UDP + a sterol 3-beta-D-glucoside
-
-
-
?
UDP-glucose + a sterol
UDP + a sterol 3-beta-D-glucoside
-
-
-
?
UDP-glucose + a sterol
UDP + a sterol 3-beta-D-glucoside
-
-
-
?
UDP-glucose + a sterol
UDP + a sterol 3-beta-D-glucoside
-
-
-
?
UDP-glucose + a sterol
UDP + a sterol 3-beta-D-glucoside
-
-
-
?
UDP-glucose + a sterol
UDP + a sterol 3-beta-D-glucoside
-
-
-
?
UDP-glucose + a sterol
UDP + a sterol 3-beta-D-glucoside
-
-
-
?
UDP-glucose + a sterol
UDP + a sterol 3-beta-D-glucoside
-
-
-
-
?
UDP-glucose + a sterol
UDP + a sterol 3-beta-D-glucoside
-
-
-
?
UDP-glucose + a sterol
UDP + a sterol 3-beta-D-glucoside
-
-
-
?
UDP-glucose + a sterol
UDP + a sterol 3-beta-D-glucoside
-
-
-
?
UDP-glucose + beta-sitosterol
UDP + beta-sitosterol 3-beta-D-glucoside
-
-
-
?
UDP-glucose + beta-sitosterol
UDP + beta-sitosterol 3-beta-D-glucoside
-
-
-
?
UDP-glucose + beta-sitosterol
UDP + beta-sitosterol 3-beta-D-glucoside
-
-
-
?
UDP-glucose + brassicasterol
UDP + brassicasterol 3-beta-D-glucoside
-
-
-
?
UDP-glucose + brassicasterol
UDP + brassicasterol 3-beta-D-glucoside
-
-
-
?
UDP-glucose + campesterol
UDP + campesterol 3-beta-D-glucoside
-
-
4% of the products
?
UDP-glucose + campesterol
UDP + campesterol 3-beta-D-glucoside
-
-
-
?
UDP-glucose + campesterol
UDP + campesterol 3-beta-D-glucoside
-
80% of the activity with poriferasterol
-
?
UDP-glucose + campesterol
UDP + campesterol 3-beta-D-glucoside
-
28% of the activity with sitosterol
-
?
UDP-glucose + campesterol
UDP + campesterol 3-beta-D-glucoside
-
best substrate
-
?
UDP-glucose + campesterol
UDP + campesterol 3-beta-D-glucoside
-
-
-
?
UDP-glucose + campesterol
UDP + campesterol 3-beta-D-glucoside
-
68% of the activity with sitosterol
-
?
UDP-glucose + cholesterol
UDP + cholesterol 3-beta-D-glucoside
-
-
-
?
UDP-glucose + cholesterol
UDP + cholesterol 3-beta-D-glucoside
cloned enzyme exhibits equal activity to the purified protein
-
?
UDP-glucose + cholesterol
UDP + cholesterol 3-beta-D-glucoside
-
-
-
?
UDP-glucose + cholesterol
UDP + cholesterol 3-beta-D-glucoside
-
with sitosterol, best substrates
-
?
UDP-glucose + cholesterol
UDP + cholesterol 3-beta-D-glucoside
cloned enzyme glucosylates cholesterol
-
?
UDP-glucose + cholesterol
UDP + cholesterol 3-beta-D-glucoside
cloned enzyme exhibits equal activity to the purified protein
-
?
UDP-glucose + cholesterol
UDP + cholesterol 3-beta-D-glucoside
cloned enzyme exhibits equal activity to the purified protein
-
?
UDP-glucose + cholesterol
UDP + cholesterol 3-beta-D-glucoside
cloned enzyme glucosylates cholesterol
-
?
UDP-glucose + cholesterol
UDP + cholesterol 3-beta-D-glucoside
-
-
-
?
UDP-glucose + cholesterol
UDP + cholesterol 3-beta-D-glucoside
-
-
-
?
UDP-glucose + cholesterol
UDP + cholesterol 3-beta-D-glucoside
cloned enzyme glucosylates cholesterol
-
?
UDP-glucose + cholesterol
UDP + cholesterol 3-beta-D-glucoside
cloned enzyme glucosylates cholesterol
-
?
UDP-glucose + cholesterol
UDP + cholesterol 3-beta-D-glucoside
-
best substrate
-
?
UDP-glucose + cholesterol
UDP + cholesterol 3-beta-D-glucoside
-
-
-
?
UDP-glucose + cholesterol
UDP + cholesterol 3-beta-D-glucoside
-
-
-
?
UDP-glucose + cholesterol
UDP + cholesterol 3-beta-D-glucoside
-
-
-
?
UDP-glucose + cholesterol
UDP + cholesterol 3-beta-D-glucoside
cloned enzyme glucosylates cholesterol
-
?
UDP-glucose + cholesterol
UDP + cholesterol 3-beta-D-glucoside
cloned enzyme glucosylates cholesterol
-
?
UDP-glucose + cholesterol
UDP + cholesterol 3-beta-D-glucoside
-
-
-
?
UDP-glucose + cholesterol
UDP + cholesterol 3-beta-D-glucoside
-
-
-
?
UDP-glucose + cholesterol
UDP + cholesterol 3-beta-D-glucoside
-
-
-
?
UDP-glucose + cholesterol
UDP + cholesterol 3-beta-D-glucoside
-
-
-
?
UDP-glucose + cholesterol
UDP + cholesterol 3-beta-D-glucoside
-
-
-
?
UDP-glucose + cholesterol
UDP + cholesterol 3-beta-D-glucoside
-
23% of the activity with sitosterol
-
?
UDP-glucose + cholesterol
UDP + cholesterol 3-beta-D-glucoside
-
12% of the activity with poriferasterol
-
?
UDP-glucose + cholesterol
UDP + cholesterol 3-beta-D-glucoside
-
-
-
?
UDP-glucose + cholesterol
UDP + cholesterol 3-beta-D-glucoside
-
-
-
?
UDP-glucose + cholesterol
UDP + cholesterol 3-beta-D-glucoside
cloned enzyme glucosylates cholesterol
-
?
UDP-glucose + cholesterol
UDP + cholesterol 3-beta-D-glucoside
-
-
-
?
UDP-glucose + cholesterol
UDP + cholesterol 3-beta-D-glucoside
-
-
-
?
UDP-glucose + ergosterol
UDP + ergosterol 3-beta-D-glucoside
-
-
-
?
UDP-glucose + ergosterol
UDP + ergosterol 3-beta-D-glucoside
-
-
-
?
UDP-glucose + ergosterol
UDP + ergosterol 3-beta-D-glucoside
cloned enzyme glucosylates ergosterol
-
?
UDP-glucose + ergosterol
UDP + ergosterol 3-beta-D-glucoside
-
69% of the activity with sitosterol
-
?
UDP-glucose + ergosterol
UDP + ergosterol 3-beta-D-glucoside
cloned enzyme glucosylates ergosterol
-
?
UDP-glucose + ergosterol
UDP + ergosterol 3-beta-D-glucoside
-
-
-
?
UDP-glucose + ergosterol
UDP + ergosterol 3-beta-D-glucoside
-
-
-
?
UDP-glucose + ergosterol
UDP + ergosterol 3-beta-D-glucoside
cloned enzyme glucosylates ergosterol
-
?
UDP-glucose + ergosterol
UDP + ergosterol 3-beta-D-glucoside
cloned enzyme glucosylates ergosterol
-
?
UDP-glucose + ergosterol
UDP + ergosterol 3-beta-D-glucoside
-
-
-
?
UDP-glucose + ergosterol
UDP + ergosterol 3-beta-D-glucoside
-
-
-
?
UDP-glucose + ergosterol
UDP + ergosterol 3-beta-D-glucoside
-
-
-
?
UDP-glucose + ergosterol
UDP + ergosterol 3-beta-D-glucoside
cloned enzyme glucosylates ergosterol
-
?
UDP-glucose + ergosterol
UDP + ergosterol 3-beta-D-glucoside
cloned enzyme glucosylates ergosterol
-
?
UDP-glucose + ergosterol
UDP + ergosterol 3-beta-D-glucoside
-
-
-
?
UDP-glucose + ergosterol
UDP + ergosterol 3-beta-D-glucoside
-
-
-
?
UDP-glucose + ergosterol
UDP + ergosterol 3-beta-D-glucoside
-
-
-
?
UDP-glucose + ergosterol
UDP + ergosterol 3-beta-D-glucoside
-
-
-
?
UDP-glucose + ergosterol
UDP + ergosterol 3-beta-D-glucoside
-
-
-
?
UDP-glucose + ergosterol
UDP + ergosterol 3-beta-D-glucoside
-
11% of the activity with sitosterol
-
?
UDP-glucose + ergosterol
UDP + ergosterol 3-beta-D-glucoside
-
9% of the activity with poriferasterol
-
?
UDP-glucose + ergosterol
UDP + ergosterol 3-beta-D-glucoside
-
-
-
?
UDP-glucose + ergosterol
UDP + ergosterol 3-beta-D-glucoside
-
-
-
?
UDP-glucose + ergosterol
UDP + ergosterol 3-beta-D-glucoside
cloned enzyme glucosylates ergosterol
-
?
UDP-glucose + ergosterol
UDP + ergosterol 3-beta-D-glucoside
-
-
-
?
UDP-glucose + ergosterol
UDP + ergosterol 3-beta-D-glucoside
-
-
-
?
UDP-glucose + estradiol
UDP + estradiol 3-beta-D-glucoside
-
-
-
?
UDP-glucose + estradiol
UDP + estradiol 3-beta-D-glucoside
-
-
-
?
UDP-glucose + estradiol
UDP + estradiol 3-beta-D-glucoside
-
-
-
?
UDP-glucose + estradiol
UDP + estradiol 3-beta-D-glucoside
-
-
-
?
UDP-glucose + estradiol
UDP + estradiol 3-beta-D-glucoside
-
-
-
?
UDP-glucose + estradiol
UDP + estradiol 3-beta-D-glucoside
-
-
-
?
UDP-glucose + estradiol
UDP + estradiol 3-beta-D-glucoside
-
-
-
?
UDP-glucose + estradiol
UDP + estradiol 3-beta-D-glucoside
-
-
-
?
UDP-glucose + estradiol
UDP + estradiol 3-beta-D-glucoside
-
-
-
?
UDP-glucose + estradiol
UDP + estradiol 3-beta-D-glucoside
-
-
-
?
UDP-glucose + estradiol
UDP + estradiol 3-beta-D-glucoside
-
-
-
?
UDP-glucose + estradiol
UDP + estradiol 3-beta-D-glucoside
-
-
-
?
UDP-glucose + estrone
UDP + estrone 3-beta-D-glucoside
-
-
-
?
UDP-glucose + estrone
UDP + estrone 3-beta-D-glucoside
-
-
-
?
UDP-glucose + estrone
UDP + estrone 3-beta-D-glucoside
-
-
-
?
UDP-glucose + estrone
UDP + estrone 3-beta-D-glucoside
-
-
-
?
UDP-glucose + estrone
UDP + estrone 3-beta-D-glucoside
-
-
-
?
UDP-glucose + estrone
UDP + estrone 3-beta-D-glucoside
-
-
-
?
UDP-glucose + estrone
UDP + estrone 3-beta-D-glucoside
-
-
-
?
UDP-glucose + estrone
UDP + estrone 3-beta-D-glucoside
-
-
-
?
UDP-glucose + estrone
UDP + estrone 3-beta-D-glucoside
-
-
-
?
UDP-glucose + estrone
UDP + estrone 3-beta-D-glucoside
-
-
-
?
UDP-glucose + estrone
UDP + estrone 3-beta-D-glucoside
-
-
-
?
UDP-glucose + estrone
UDP + estrone 3-beta-D-glucoside
-
-
-
?
UDP-glucose + pregnenolone
UDP + pregnenolone 3-beta-D-glucoside
-
-
-
-
?
UDP-glucose + pregnenolone
UDP + pregnenolone 3-beta-D-glucoside
-
-
-
?
UDP-glucose + protopanaxadiol
UDP + protopanaxadiol 3-beta-D-glucoside
an unnatural substrate of enzyme mutant 7_1
-
-
?
UDP-glucose + protopanaxadiol
UDP + protopanaxadiol 3-beta-D-glucoside
an unnatural substrate of enzyme mutant 7_1
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UDP-glucose + sitosterol
UDP + sitosterol 3-beta-D-glucoside
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UDP-glucose + sitosterol
UDP + sitosterol 3-beta-D-glucoside
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UDP-glucose + sitosterol
UDP + sitosterol 3-beta-D-glucoside
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UDP-glucose + sitosterol
UDP + sitosterol 3-beta-D-glucoside
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UDP-glucose + sitosterol
UDP + sitosterol 3-beta-D-glucoside
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UDP-glucose + sitosterol
UDP + sitosterol 3-beta-D-glucoside
cloned enzyme glucosylates sitosterol
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UDP-glucose + sitosterol
UDP + sitosterol 3-beta-D-glucoside
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with cholesterol, best substrates
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UDP-glucose + sitosterol
UDP + sitosterol 3-beta-D-glucoside
cloned enzyme glucosylates sitosterol
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UDP-glucose + sitosterol
UDP + sitosterol 3-beta-D-glucoside
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UDP-glucose + sitosterol
UDP + sitosterol 3-beta-D-glucoside
cloned enzyme glucosylates sitosterol
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UDP-glucose + sitosterol
UDP + sitosterol 3-beta-D-glucoside
cloned enzyme glucosylates sitosterol
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UDP-glucose + sitosterol
UDP + sitosterol 3-beta-D-glucoside
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88% of the activity with epiandrosterone
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UDP-glucose + sitosterol
UDP + sitosterol 3-beta-D-glucoside
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UDP-glucose + sitosterol
UDP + sitosterol 3-beta-D-glucoside
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75% of the product
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UDP-glucose + sitosterol
UDP + sitosterol 3-beta-D-glucoside
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UDP-glucose + sitosterol
UDP + sitosterol 3-beta-D-glucoside
cloned enzyme glucosylates sitosterol
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UDP-glucose + sitosterol
UDP + sitosterol 3-beta-D-glucoside
cloned enzyme glucosylates sitosterol
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UDP-glucose + sitosterol
UDP + sitosterol 3-beta-D-glucoside
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best substrate
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UDP-glucose + sitosterol
UDP + sitosterol 3-beta-D-glucoside
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maximal activity, the same as poriferasterol
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UDP-glucose + sitosterol
UDP + sitosterol 3-beta-D-glucoside
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greater activity than with other sterols
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UDP-glucose + sitosterol
UDP + sitosterol 3-beta-D-glucoside
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UDP-glucose + sitosterol
UDP + sitosterol 3-beta-D-glucoside
cloned enzyme glucosylates sitosterol
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UDP-glucose + sitosterol
UDP + sitosterol 3-beta-D-glucoside
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UDP-glucose + sitosterol
UDP + sitosterol 3-beta-D-glucoside
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UDP-glucose + sitosterol
UDP + sitosterol 3-beta-D-glucoside
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UDP-glucose + sitosterol
UDP + sitosterol 3-beta-D-glucoside
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best substrate
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UDP-glucose + stigmasterol
UDP + stigmasterol 3-beta-D-glucoside
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UDP-glucose + stigmasterol
UDP + stigmasterol 3-beta-D-glucoside
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UDP-glucose + stigmasterol
UDP + stigmasterol 3-beta-D-glucoside
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UDP-glucose + stigmasterol
UDP + stigmasterol 3-beta-D-glucoside
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UDP-glucose + stigmasterol
UDP + stigmasterol 3-beta-D-glucoside
cloned enzyme glucosylates stigmasterol
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UDP-glucose + stigmasterol
UDP + stigmasterol 3-beta-D-glucoside
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63% of the activity with sitosterol
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UDP-glucose + stigmasterol
UDP + stigmasterol 3-beta-D-glucoside
cloned enzyme glucosylates stigmasterol
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UDP-glucose + stigmasterol
UDP + stigmasterol 3-beta-D-glucoside
cloned enzyme glucosylates stigmasterol
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UDP-glucose + stigmasterol
UDP + stigmasterol 3-beta-D-glucoside
cloned enzyme glucosylates stigmasterol
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UDP-glucose + stigmasterol
UDP + stigmasterol 3-beta-D-glucoside
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122% of the activity with epiandrosterone, highest specificity
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UDP-glucose + stigmasterol
UDP + stigmasterol 3-beta-D-glucoside
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6% of the product
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UDP-glucose + stigmasterol
UDP + stigmasterol 3-beta-D-glucoside
cloned enzyme glucosylates stigmasterol
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UDP-glucose + stigmasterol
UDP + stigmasterol 3-beta-D-glucoside
cloned enzyme glucosylates stigmasterol
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UDP-glucose + stigmasterol
UDP + stigmasterol 3-beta-D-glucoside
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14% of the activity with poriferasterol
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UDP-glucose + stigmasterol
UDP + stigmasterol 3-beta-D-glucoside
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50% of the activity with sitosterol
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UDP-glucose + stigmasterol
UDP + stigmasterol 3-beta-D-glucoside
cloned enzyme glucosylates stigmasterol
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UDP-glucose + stigmasterol
UDP + stigmasterol 3-beta-D-glucoside
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UDP-glucose + stigmasterol
UDP + stigmasterol 3-beta-D-glucoside
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UDP-glucose + stigmasterol
UDP + stigmasterol 3-beta-D-glucoside
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UDP-glucose + tomatidine
UDP + tomatidine 3-beta-glucoside
cloned enzyme glucosylates tomatidine
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UDP-glucose + tomatidine
UDP + tomatidine 3-beta-glucoside
cloned enzyme glucosylates tomatidine
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UDP-glucose + tomatidine
UDP + tomatidine 3-beta-glucoside
cloned enzyme glucosylates tomatidine
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UDP-glucose + tomatidine
UDP + tomatidine 3-beta-glucoside
cloned enzyme glucosylates tomatidine
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UDP-glucose + transandrosterone
UDP + transandrosterone 3beta-D-glucoside
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UDP-glucose + transandrosterone
UDP + transandrosterone 3beta-D-glucoside
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additional information
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the enzyme only shows activity with sterols having hydroxyl group at C-3 position. Utilises only UDP-glucose, UDP-galactose cannot serve as the sugar donor.
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additional information
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yamogenin is not substrate of this enzyme
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additional information
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UDP-glucose is an effective glucose donor but not so UDP-mannose, UDP-galactose or UMP-glucose
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additional information
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UDP-glucose best glucose donor, CDP-glucose, GDP-glucose and UDP-xylopyranose are used but at significantly lower rates and UDP-mannose, UDP-glucaronic acid, GDP-mannose and TDP-glucose are not effective glucose donor
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additional information
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UDP-glucose best glucose donor, CDP-glucose, GDP-glucose and UDP-xylopyranose are used but at significantly lower rates and UDP-mannose, UDP-glucaronic acid, GDP-mannose and TDP-glucose are not effective glucose donor
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additional information
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the enzyme is a fungal virulence factor
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additional information
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UDP-glucose best glucose donor, CDP-glucose, GDP-glucose and UDP-xylopyranose are used but at significantly lower rates and UDP-mannose, UDP-glucaronic acid, GDP-mannose and TDP-glucose are not effective glucose donor
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additional information
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UDP-glucose best glucose donor, CDP-glucose, GDP-glucose and UDP-xylopyranose are used but at significantly lower rates and UDP-mannose, UDP-glucaronic acid, GDP-mannose and TDP-glucose are not effective glucose donor
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additional information
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UDP-glucose best glucose donor, CDP-glucose, GDP-glucose and UDP-xylopyranose are used but at significantly lower rates and UDP-mannose, UDP-glucaronic acid, GDP-mannose and TDP-glucose are not effective glucose donor
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additional information
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almost no activity with androsterone, digitoxigenin, digitoxin and testosterone
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additional information
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UDP-glucose is an effective glucose donor but not so ADP-glucose, CDP-glucose, TDP-glucose, GDP-glucose, UDP-galactose or UDP-mannose
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additional information
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UDP-glucose best glucose donor, CDP-glucose, GDP-glucose and UDP-xylopyranose are used but at significantly lower rates and UDP-mannose, UDP-glucaronic acid, GDP-mannose and TDP-glucose are not effective glucose donor
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additional information
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UDP-glucose best glucose donor, CDP-glucose, GDP-glucose and UDP-xylopyranose are used but at significantly lower rates and UDP-mannose, UDP-glucaronic acid, GDP-mannose and TDP-glucose are not effective glucose donor
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additional information
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sterol glucosyltransferase Ugt51/Paz4 is essential for pexophagy (peroxisome degradation), but not for macroautophagy in the methylotrophic yeast. Deletion of the catalytic domain does not impair protein localization, but abolishes pexophagy, suggesting that SG synthesis is required for this process
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additional information
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sterol glucosyltransferase Ugt51/Paz4 is essential for pexophagy (peroxisome degradation), but not for macroautophagy in the methylotrophic yeast. Deletion of the catalytic domain does not impair protein localization, but abolishes pexophagy, suggesting that SG synthesis is required for this process
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additional information
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the UGT51 gene is required for pexophagy and other vacuole related processes
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additional information
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sterol glucosyltransferase Ugt51/Paz4 is essential for pexophagy (peroxisome degradation), but not for macroautophagy in the methylotrophic yeast. Deletion of the catalytic domain does not impair protein localization, but abolishes pexophagy, suggesting that SG synthesis is required for this process
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additional information
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UDP-glucose best glucose donor, CDP-glucose, GDP-glucose and UDP-xylopyranose are used but at significantly lower rates and UDP-mannose, UDP-glucaronic acid, GDP-mannose and TDP-glucose are not effective glucose donor
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additional information
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DELTA5-sterols are glucosylated at higher rate than DELTA7-sterols
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additional information
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glucose 1-phosphate, GDP-glucose and ADP-glucose are inactive substrates
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additional information
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presence and localization of double bonds in the ring system exert a pronounced effect on the rate of glucosylation
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additional information
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the presence of a DELTA22 double bond decreases affinity of the sterol for the enzyme
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additional information
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enzyme can use CDP-glucose and TDP-glucose at 6 times slower rate
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additional information
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coprostanol and epicholestanol are not glucosylated
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additional information
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sterols possessing an alkyl group at C-24 are better substrates than C27 sterols
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additional information
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stanols and sterols with conjugated double bonds in ring B are poor substrates
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additional information
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all 3-B-OH sterols containing a double bond in position 5 are glucosylated and a double bond at C24 reduces glucosylation
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additional information
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UDP-glucose is an effective glucose donor, not so ADP-glucose, CDP-glucose or GDP-glucose
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additional information
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UDP-glucose best glucose donor, CDP-glucose, GDP-glucose and UDP-xylopyranose are used but at significantly lower rates and UDP-mannose, UDP-glucaronic acid, GDP-mannose and TDP-glucose are not effective glucose donor
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additional information
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UDP-glucose best glucose donor, CDP-glucose, GDP-glucose and UDP-xylopyranose are used but at significantly lower rates and UDP-mannose, UDP-glucaronic acid, GDP-mannose and TDP-glucose are not effective glucose donor
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additional information
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experimental results suggest that the enzyme is not involved in the cytoplasm to vacuole targeting pathway, macroautophagy and pexophagy
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additional information
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experimental results suggest that the enzyme is not involved in the cytoplasm to vacuole targeting pathway, macroautophagy and pexophagy
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additional information
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substrate molecular docking and structure analysis of wild-type and mutant enzymes, sugar donor binding mechanism of UGT51, overview
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additional information
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substrate molecular docking and structure analysis of wild-type and mutant enzymes, sugar donor binding mechanism of UGT51, overview
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additional information
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substrate molecular docking and structure analysis of wild-type and mutant enzymes, sugar donor binding mechanism of UGT51, overview
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additional information
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enzyme is absolute specific to UDP-glucose
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additional information
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glucose 1-phosphate, GDP-glucose and ADP-glucose are inactive substrates
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additional information
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all 3-B-OH sterols containing a double bond in position 5 are glucosylated and a double bond at C24 reduces glucosylation
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additional information
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SGT activity assays are carried out with a plant sterol mixture consisting of approximately 13% brassicasterol, 26% campesterol, 7% stigmasterol, and 53% beta-sitosterol in ethanol, and UDP-glucose
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additional information
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SGT activity assays are carried out with a plant sterol mixture consisting of approximately 13% brassicasterol, 26% campesterol, 7% stigmasterol, and 53% beta-sitosterol in ethanol, and UDP-glucose
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additional information
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SGT activity assays are carried out with a plant sterol mixture consisting of approximately 13% brassicasterol, 26% campesterol, 7% stigmasterol, and 53% beta-sitosterol in ethanol, and UDP-glucose
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additional information
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SGT activity assays are carried out with a plant sterol mixture consisting of approximately 13% brassicasterol, 26% campesterol, 7% stigmasterol, and 53% beta-sitosterol in ethanol, and UDP-glucose
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additional information
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SGT activity assays are carried out with a plant sterol mixture consisting of approximately 13% brassicasterol, 26% campesterol, 7% stigmasterol, and 53% beta-sitosterol in ethanol, and UDP-glucose
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additional information
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may participate in the regulating of free sterol concentration in the cell
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additional information
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UDP-galactose, TDP-glucose and CDP-glucose are also suitable as a sugar source for sterol glucosylation but with distinctly lower rates
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additional information
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no activity with solasodine (25R-22alphaN-spirosol-5-enin-3beta-ol), solanidine (22S,25S-solanid-5-enin-3beta-ol), sarsasapogenin (25S-5beta-spirostan-3beta-ol), yamogenin (25S-spirost-5-en-3beta-ol), thiocholesterol (cholest-5-en-3beta-tiol), 5beta-cholestan-3beta-ol (coprostanol), 5alpha-cholestan-3alpha-ol (epicholestanol), 5beta-cholestan-3alpha-ol (epicoprostanol), lanosterol (4,4,14-trimethyl-5alpha-cholest-8-en-3beta-ol), and beta-amyrin (olean-12-en-3beta-ol) do not serve as glucose acceptors
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additional information
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the enzyme is highly specific to sterols and shows no activity with non steroidal substrates. Amongst the sterols, the enzyme is position specific and works only with C-3 hydroxyl sterols, but the extradiol C-3 hydroxyl group is not glucosylated. Sterols with A-B ring 5-en (e.g. deacetyl-16-DPA) are glucosylated more efficiently than those with 5alpha-H. For the cytosolic enzyme, the relative substrate specificity of the membrane-bound enzyme is different. The membrane enzyme is more specific towards phytosterols and brassicasterol. It shows no activity towards secondary metabolits and flavonoids and isoflavonoids.
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additional information
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the enzyme only shows activity with sterols having hydroxyl group at C-3 position. SGTL1 is more active toward sterols without side chain. Utilises only UDP-glucose, UDP-galactose can not serve as the sugar donor.
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additional information
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the enzyme only shows activity with sterols having hydroxyl group at C-3 position. SGTL1 is more active toward sterols without side chain. Utilises only UDP-glucose, UDP-galactose can not serve as the sugar donor.
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additional information
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the enzyme is specific for the 3beta-hydroxy position and has a catalytic specificity to glycosylate withanolide and sterols
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additional information
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the enzyme is specific for the 3beta-hydroxy position and has a catalytic specificity to glycosylate withanolide and sterols
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additional information
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the UGT51 gene is required for utilization of decane, but not for pexophagy
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additional information
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p-nitrophenol, estrone and 4-alpha,10-beta-dimethyl-trans-decal-3-beta-ol are not substrates
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stigmast-5-en-3beta-ol + UDP-glucose
additional information
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sitosterol, 100% of the activity
3-O-beta-D-monoglucopyranoside of sitosterol and 3-O-beta-D-monogalactopyranoside of sitosterol identified as products
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