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2.4.1.175: glucuronosyl-N-acetylgalactosaminyl-proteoglycan 4-beta-N-acetylgalactosaminyltransferase

This is an abbreviated version!
For detailed information about glucuronosyl-N-acetylgalactosaminyl-proteoglycan 4-beta-N-acetylgalactosaminyltransferase, go to the full flat file.

Word Map on EC 2.4.1.175

Reaction

UDP-N-acetyl-alpha-D-galactosamine
+
[protein]-3-O-(beta-D-GlcA-(1->3)-beta-D-GalNAc-(1->4)-beta-D-GlcA-(1->3)-beta-D-Gal-(1->3)-beta-D-Gal-(1->4)-beta-D-Xyl)-L-serine
=
UDP
 +
[protein]-3-O-(beta-D-GalNAc-(1->4)-beta-D-GlcA-(1->3)-beta-D-GalNAc-(1->4)-beta-D-GlcA-(1->3)-beta-D-Gal-(1->3)-beta-D-Gal-(1->4)-beta-D-Xyl)-L-serine

Synonyms

AcbD, acetylgalactosaminyltransferase, uridine diphosphoacetylgalactosamine-chondroitin, II, ApCS, bifunctional CS synthase, ChGn-1, ChGn-2, chondroitin galactosaminyltransferase, chondroitin N-acetylgalactosaminyltransferase-1, chondroitin N-acetylgalactosaminyltransferase-2, chondroitin polymerase, chondroitin sulfate beta-1,4-N-acetylgalactosaminyltransferase-1, chondroitin sulfate glucuronyltransferase, chondroitin sulfate glycosyltransferase, chondroitin sulfate N-acetylgalactosaminyltransferase-1, chondroitin sulfate N-acetylgalactosaminyltransferase-2, chondroitin sulfate synthase 1, chondroitin sulfate synthase-1, chondroitin sulfate synthase-2, chondroitin sulfate synthase-2/chondroitin polymerizing factor, chondroitin sulfate synthase-3, chondroitin synthase, chondroitin synthase 1, chondroitin synthase-1, chondroitin synthase-2, chondroitin synthase-3, CHPF, ChSy, ChSy-1, ChSy-2, ChSy-3, CHSY1, CpCS, Cpha266_2341, CS glycosyltransferase, CS N-acetylgalactosaminyltransferase-1, CS synthase 1, CS synthase-1, CSGalNAcT-2, CSGlcA-T, CSS1, CSS2, CSS3, GalNAc-T, glucuronyl-N-acetylgalactosaminylproteoglycan beta1,4-N-acetylgalactosaminyltransferase, K4CP, More, N-acetylgalactosaminyltransferase II, UDP-N-acetyl-D-galactosamine:D-glucuronyl-N-acetyl-1,3-beta-D-galactosaminylproteoglycan beta-1,4-N-acetylgalactosaminyltransferase, uridine diphosphoacetylgalactosamine-chondroitin acetylgalactosaminyltransferasec II

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.175 glucuronosyl-N-acetylgalactosaminyl-proteoglycan 4-beta-N-acetylgalactosaminyltransferase

Cloned

Cloned on EC 2.4.1.175 - glucuronosyl-N-acetylgalactosaminyl-proteoglycan 4-beta-N-acetylgalactosaminyltransferase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
ChGn-2, expression analysis by quantitative real-time RT-PCR, co-expression of ChGn-1 or ChGn-2 into sog9 and L cells, sog9 is a mutant L cell line are deficient in the expression of chondroitin 4-O-sulfotransferase-1, C4ST-1, disaccharide composition of chondroitin sulfate in control and transfected L cells, overview
-
CSS2 quantitative expression analysis by realtime RT-PCR, overview
-
DNA and amino acid sequence determination and analysis, expression as soluble His-tagged protein in Escherichia coli strain TOP10
DNA sequence determination and analysis, expression of a truncated form, consisting of the putative catalytic domain, in Spodoptera frugiperda Sf21 insect cells as a soluble FLAG-tagged protein
DNA sequence determination, expression in Escherichia coli of amino acid residues 1-704 of total 965 as soluble functional protein
DNA sequence determination, genomic organization and chromosome localization, functional expression as soluble protein fused to protein A IgG-binding domain in COS-1 cells, secretion into the medium
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia Top10 cells
-
expressed in HeLa cells
expression in COS cells
gene acbD, encoded within the presumed capsule biosynthesis gene cluster, functional recombinant expression of N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3) as soluble protein
gene ChSy-1, genotyping in healthy persons and multiple sclerosis patients, recombinant expression of wild-type and mutant enzymes in COS-1 cells
gene CHSY1, quantitative reverse-transcription PCR enzyme expression analysis
gene CHSY1, quantitative RT-PCR enzyme expression analysis
gene CHSY1, quantitative RT-PCR enzyme expressiona analysis
gene chsy1, real-time PCR enzyme expression analysis
-
gene CHSY1, soluble forms of wild-type and mutant ChSy-1 is heterologously expressed in COS-1 cells, the recombinant enzymes are secreted to the medium, quantitative real-time RT-PCR enzyme expression analysis
gene Cpha266_2341, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of MBP-tagged enzyme in Escherichia coli strain XJa from plasmid CpCS/pMAL-c4E
genomic organization and chromosome localization, functional expression as soluble protein fused to protein A IgG-binding domain in COS-1 cells, secretion into the medium
GFP-tagged and DsRed-monomer tagged enzyme expressed in HeLa cells
individual overexpression of soluble forms of FLAG- and Myc-tagged splice variants CSS2A and CSS2B in COS-7 cells, co-expression with CSS1, EC 2.4.1.174. Whereas CSS2A facilitates chondroitin sulfate biosynthesis, CSS2B inhibits it. Analysis of transcription levels of CSS2 variants
-
soluble form of the enzyme lacking the first 129 amino acids expressed as protein A fusion protein in COS-1 cells
soluble form of the enzyme lacking the first 57 amino acids expressed as protein A fusion protein in COS-1 cells