complete cDNA sequence for the wild-type gene and the nonsense mutation in which a C to A substitution at base 102 results in a tyrosine (Y) to stop (X) mutation in codon 34 of exon of exon 1
expression in Escherichia coli. Multiple forms of the enzyme exist which differ mainly in the length of a polyglutamic acid repeat at the C-terminus of the protein, SBE A1 to SBE A-6. Expression of an antisense SBE A RNA in transgenic potato
expression of Escherichia coli branching enzyme in caryopses of transgenic Oryza satica results in amylopectin with an increased degree of branching. Expression of Escgerichia coli glgB in rice results in an increase in the short-chain fractions with DP between 6 and 10, which should have a significant effect on retrogradation
gene glgB, functional recombinant expression of the bacterial enzyme in Arabidopsis thaliana be2 be3 double mutant leaves, ecotype WS, which is devoid of BE activity and consequently free of starch. The synthesis of a water-insoluble, partly crystalline, amylose-containing starch-like polyglucan is restored in GlgB-expressing transgenic plants, morphology of purified insoluble and soluble polyglucans, phenotype, overview
gene RmGBE, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of N-terminally His-tagged enzyme in Escherichia coli strain BL21
gene SBE I, DNA and amino acid sequence determination and analysis of SBE I from two accessions, Y59 and Y63, genetic structure, sequence comparisons and phylogenetic analysis, enzyme expression analysis by real-time quantitative PCR
recombinant overexpression of endogenaous enzyme SBEII in Solanum tuberosum. A complete cDNA of potato SBEII is unable to be propagated in Escherichia coli, therefore a complete but hybrid SBEII intragene containing a single intron to prevent bacterial translation is assembled from cDNA and genomic DNA fragments
the gene encoding the branching enzyme from Anaerobranca gottschalkii is fused with a twin arginine translocation protein secretory-pathway-dependent siganl sequence from Escherichia coli and expressed in Staphylococcus carnosus