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2.4.1.18: 1,4-alpha-glucan branching enzyme

This is an abbreviated version!
For detailed information about 1,4-alpha-glucan branching enzyme, go to the full flat file.

Word Map on EC 2.4.1.18

Reaction

2 1,4-alpha-D-glucan =

alpha-1,4-D-glucan-alpha-1,6-(alpha-1,4-D-glucan)
+
H2O

Synonyms

(1-4)-alpha-D-glucan:(1-4)-alpha-D-glucan 6-alpha-D-[(1-4)-alpha-D-glucano]-transferase, 1,4-alpha-glucan branching enzyme, 1,4-alpha-glucan branching enzyme 1, AGBE, alpha-1,4->alpha-1,6-glycosyltransferase, alpha-1,4-glucan branching enzyme, alpha-1,4-glucan:alpha-1,4-glucan 6-glycosyltransferase, alpha-1,4-glucan:alpha-1,4-glucan-6-glycosyltransferase, alpha-glucan branching enzyme, alpha-glucan-branching glycosyltransferase, amylo-(1,4-1,6)-transglycosylase, amylo-(1,4->1,6)-transglycosylase, amylose isomerase, BBE, BE, BE-01, BE-02, BE-II, BE1, BE2, BE3, BEI, BEII, BEIIa, BEIIb, BGE, branching enzyme, branching factor, enzymatic, branching glycosyltransferase, ChlBE, Dg GBE, Dr GBE, enzyme Q, full-length starch branching enzyme II, GBE, GBE1, GBE1 enzyme, GH13 GBE, GH57-type glycogen branching enzyme, GlgB, glucan transferase, glucosan transglycosylase, glycogen branching enzyme, glycogen branching enzyme GBE1, glycosyltransferase, alpha-glucan-branching, HosBE, IbSBEI, mSBEIIa, ORF Rv1326c, PH1386, PhGBE, PvSBE1, PvSBE2, Q-enzyme, QE, rBEI, rice branching enzyme I, RMEBE, RoBE, SBE, SBE A, SBE B, SBE I, SBE IIa, SBE Iib, SBE-I, SBE1, SBEI, SBEII, SBEIIA, SBEIIB, ScoBE, SmGBE, starch branching enzyme, starch branching enzyme I, starch branching enzyme II, starch branching enzyme IIa, starch branching enzyme IIb, starch branching enzyme SBE I, starch branching enzyme SBE IIb, starch branching enzyme-I, starch-branching enzyme, starch-branching enzyme Ia, starch-branching enzyme IIa, starch-branching enzyme IIb, starch-branching enzymes IIa, starch-branching enzymes IIb, starch-branching-enzyme, TK1436, VsbeII

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.18 1,4-alpha-glucan branching enzyme

Cloned

Cloned on EC 2.4.1.18 - 1,4-alpha-glucan branching enzyme

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
complete cDNA sequence for the wild-type gene and the nonsense mutation in which a C to A substitution at base 102 results in a tyrosine (Y) to stop (X) mutation in codon 34 of exon of exon 1
expressed in Escherichia coli
-
expressed in Escherichia coli BL21 (DE3)
-
expressed in Escherichia coli BL21 cells
expressed in Escherichia coli BL21 Star (DE3) cells
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli Bl21-CodonPlus(DE3)-RIL and pET21a(+)
expressed in Escherichia coli DG5alpha
expressed in Escherichia coli strain KV832
expression in Bacillus subtilis
expression in Escherichia coli
expression in Escherichia coli AD494
-
expression in Escherichia coli BL21
expression in Escherichia coli, chimeric enzymes consisting of part mBE I and mBE II
-
expression in Escherichia coli, full-length enzyme and a truncated enzyme form missing the first 107 amino acids
-
expression in Escherichia coli, glycogen branching enzyme gene existing in tandem with the glycogen debranching enzyme
-
expression in Escherichia coli. Multiple forms of the enzyme exist which differ mainly in the length of a polyglutamic acid repeat at the C-terminus of the protein, SBE A1 to SBE A-6. Expression of an antisense SBE A RNA in transgenic potato
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expression in mosquito C6/36 cells
expression in Saccharomyces cerevisiae, each of the three isoforms is functional in yeast cells
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expression of Escherichia coli branching enzyme in caryopses of transgenic Oryza satica results in amylopectin with an increased degree of branching. Expression of Escgerichia coli glgB in rice results in an increase in the short-chain fractions with DP between 6 and 10, which should have a significant effect on retrogradation
-
expression of mSBEIIa in Escherichia coli as Strep-tagged enzyme
gene gkgB, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
-
gene glgB, functional recombinant expression of the bacterial enzyme in Arabidopsis thaliana be2 be3 double mutant leaves, ecotype WS, which is devoid of BE activity and consequently free of starch. The synthesis of a water-insoluble, partly crystalline, amylose-containing starch-like polyglucan is restored in GlgB-expressing transgenic plants, morphology of purified insoluble and soluble polyglucans, phenotype, overview
gene nbrc_glgB1, functional recombinant expression of N-terminally His-tagged isozyme BE1 in Escherichia coli
Cyanobacterium sp.
gene nbrc_glgB2, functional recombinant expression of His-tagged isozyme BE2 in Escherichia coli
Cyanobacterium sp.
gene nbrc_glgB3, functional recombinant expression of His-tagged isozyme BE3 in Escherichia coli
Cyanobacterium sp.
gene RmGBE, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of N-terminally His-tagged enzyme in Escherichia coli strain BL21
gene SBE I, DNA and amino acid sequence determination and analysis of SBE I from two accessions, Y59 and Y63, genetic structure, sequence comparisons and phylogenetic analysis, enzyme expression analysis by real-time quantitative PCR
gene Sbe2a, recombinant expression of N-terminally His6-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)pLysS
gene TcGBE, recombinant expression in Escherichia coli strain BL21(DE3)
-
isoenzymes PySBE1 and PySBE2, expression in Escherichia coli
-
isolation and characterization of a starch-branching enzymes IIa cDNA
-
isolation of a cDNA encoding a granule-bound 152-kilodalton starch-branching enzyme, a non-full-length cDNA clone
-
isolation of wheat SBE IIb cDNA
overexpression in Escherichia coli BL21
recombinant expression of His-tagged isozymes rBE2 and rBE3 in Escherichia coli strain BL21(DE3) and in strain DELTAglgCAP with BL21(DE3) background
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recombinant expression of wild-type and mutant enzymes
recombinant overexpression of endogenaous enzyme SBEII in Solanum tuberosum. A complete cDNA of potato SBEII is unable to be propagated in Escherichia coli, therefore a complete but hybrid SBEII intragene containing a single intron to prevent bacterial translation is assembled from cDNA and genomic DNA fragments
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the barley clone encodes the complete mature SBEI but is truncated by approximately 150 bases at the 5'-end
the gene encoding the branching enzyme from Anaerobranca gottschalkii is fused with a twin arginine translocation protein secretory-pathway-dependent siganl sequence from Escherichia coli and expressed in Staphylococcus carnosus
transformed to Agrobacterium tumefaciens EHA 105, generation of transgenic Oryza sativa, expression in Escherichia coli AD494
two closely related cDNAs encode starch branching enzyme from Arabidopsis thaliana
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wild-type and mutant enzymes R384A, R384S, R384Q, E384E and R384K
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