Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

2.4.1.18: 1,4-alpha-glucan branching enzyme

This is an abbreviated version!
For detailed information about 1,4-alpha-glucan branching enzyme, go to the full flat file.

Word Map on EC 2.4.1.18

Reaction

2 1,4-alpha-D-glucan =

alpha-1,4-D-glucan-alpha-1,6-(alpha-1,4-D-glucan)
+
H2O

Synonyms

(1-4)-alpha-D-glucan:(1-4)-alpha-D-glucan 6-alpha-D-[(1-4)-alpha-D-glucano]-transferase, 1,4-alpha-glucan branching enzyme, 1,4-alpha-glucan branching enzyme 1, AGBE, alpha-1,4->alpha-1,6-glycosyltransferase, alpha-1,4-glucan branching enzyme, alpha-1,4-glucan:alpha-1,4-glucan 6-glycosyltransferase, alpha-1,4-glucan:alpha-1,4-glucan-6-glycosyltransferase, alpha-glucan branching enzyme, alpha-glucan-branching glycosyltransferase, amylo-(1,4-1,6)-transglycosylase, amylo-(1,4->1,6)-transglycosylase, amylose isomerase, BBE, BE, BE-01, BE-02, BE-II, BE1, BE2, BE3, BEI, BEII, BEIIa, BEIIb, BGE, branching enzyme, branching factor, enzymatic, branching glycosyltransferase, ChlBE, Dg GBE, Dr GBE, enzyme Q, full-length starch branching enzyme II, GBE, GBE1, GBE1 enzyme, GH13 GBE, GH57-type glycogen branching enzyme, GlgB, glucan transferase, glucosan transglycosylase, glycogen branching enzyme, glycogen branching enzyme GBE1, glycosyltransferase, alpha-glucan-branching, HosBE, IbSBEI, mSBEIIa, ORF Rv1326c, PH1386, PhGBE, PvSBE1, PvSBE2, Q-enzyme, QE, rBEI, rice branching enzyme I, RMEBE, RoBE, SBE, SBE A, SBE B, SBE I, SBE IIa, SBE Iib, SBE-I, SBE1, SBEI, SBEII, SBEIIA, SBEIIB, ScoBE, SmGBE, starch branching enzyme, starch branching enzyme I, starch branching enzyme II, starch branching enzyme IIa, starch branching enzyme IIb, starch branching enzyme SBE I, starch branching enzyme SBE IIb, starch branching enzyme-I, starch-branching enzyme, starch-branching enzyme Ia, starch-branching enzyme IIa, starch-branching enzyme IIb, starch-branching enzymes IIa, starch-branching enzymes IIb, starch-branching-enzyme, TK1436, VsbeII

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.18 1,4-alpha-glucan branching enzyme

Purification

Purification on EC 2.4.1.18 - 1,4-alpha-glucan branching enzyme

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
2 isozymes
25.4fold after immobilized metal-ion affinity chromatography
3.45fold after Ni-NTA affinity chromatography
-
4 isozymes
-
about 19.0 to 101.9fold after Sephacryl S-200 gel filtration and Q2-sepharose anion exchange chromatography
-
ammonium sulfate, Q-Sepharose column, ultrafiltration, gel filtration with Superdex 200 column, native polyacrylamide gel elektrophoresis
-
anion exchange chromatography
-
anion exchange column and hydrophobic column
chimeric and N-terminal truncated enzymes: DEAE-Sepharose Cl6B column, Bio-Gel P-200 and Gigapite column all at 4°C and pH 7.5. Site-directed mutant enzymes: chelating Sepharose FF column and DEAE-Sepharose CL-6B column, all at 4°C.
-
chimeric enzymes expressed in E. coli
-
His tag affinity chromatography
His Trap column chromatography
-
His-tag affinity column chromatography
HisTrap column chromatography
HiTrap ANX column chromatography
HiTrap ANX column chromatography and HiTrap phenyl column chromatography
HiTrap chelating column chromatography
HiTrap column chromatography and Superdex S200 gel filtration
HiTrap phenyl column chromatography and HiTrap ANX column chromatography
HitrapQ HP anion exchange column
isoform QEIIf
-
isoform RBE 4
-
large scale purification from white potato tuber
-
Ni-NTA agarose column chromatography
Ni-NTA column chromatography
Ni-NTA resin chelating column chromatography
-
Ni2+-NTA affinity column and Q-Sepharose column
partial, 800000 Da enzyme form
-
Phenyl Sepharose 6 Fast Flow column and Superdex 200 gel filtration column
Q-sepharose chromatography
recombinant enzyme from Escherichia coli strain BL21(DE3)
-
recombinant His-tagged isozyme BE1 from Escherichia coli by nickel affinity chromatography, gel filtration, and ultrafiltration
Cyanobacterium sp.
recombinant His-tagged isozyme BE2 from Escherichia coli by nickel affinity chromatography, gel filtration, and ultrafiltration
Cyanobacterium sp.
recombinant His-tagged isozyme BE3 from Escherichia coli by nickel affinity chromatography, gel filtration, and ultrafiltration
Cyanobacterium sp.
recombinant His-tagged isozymes rBE2 and rBE3 from Escherichia coli strains by nickel affinity chromatography
-
recombinant N-terminally His-tagged enzyme from Escherichia coli strain BL21 by nickel affinity chromatography
recombinant N-terminally His6-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3)pLysS by nickel affinity chromatography
wild-type and mutant enzymes R384A, R384S, R384Q, E384E and R384K
-
wild-type and truncation mutants
-