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2.4.1.18: 1,4-alpha-glucan branching enzyme

This is an abbreviated version!
For detailed information about 1,4-alpha-glucan branching enzyme, go to the full flat file.

Word Map on EC 2.4.1.18

Reaction

2 1,4-alpha-D-glucan =

alpha-1,4-D-glucan-alpha-1,6-(alpha-1,4-D-glucan)
+
H2O

Synonyms

(1-4)-alpha-D-glucan:(1-4)-alpha-D-glucan 6-alpha-D-[(1-4)-alpha-D-glucano]-transferase, 1,4-alpha-glucan branching enzyme, 1,4-alpha-glucan branching enzyme 1, AGBE, alpha-1,4->alpha-1,6-glycosyltransferase, alpha-1,4-glucan branching enzyme, alpha-1,4-glucan:alpha-1,4-glucan 6-glycosyltransferase, alpha-1,4-glucan:alpha-1,4-glucan-6-glycosyltransferase, alpha-glucan branching enzyme, alpha-glucan-branching glycosyltransferase, amylo-(1,4-1,6)-transglycosylase, amylo-(1,4->1,6)-transglycosylase, amylose isomerase, BBE, BE, BE-01, BE-02, BE-II, BE1, BE2, BE3, BEI, BEII, BEIIa, BEIIb, BGE, branching enzyme, branching factor, enzymatic, branching glycosyltransferase, ChlBE, Dg GBE, Dr GBE, enzyme Q, full-length starch branching enzyme II, GBE, GBE1, GBE1 enzyme, GH13 GBE, GH57-type glycogen branching enzyme, GlgB, glucan transferase, glucosan transglycosylase, glycogen branching enzyme, glycogen branching enzyme GBE1, glycosyltransferase, alpha-glucan-branching, HosBE, IbSBEI, mSBEIIa, ORF Rv1326c, PH1386, PhGBE, PvSBE1, PvSBE2, Q-enzyme, QE, rBEI, rice branching enzyme I, RMEBE, RoBE, SBE, SBE A, SBE B, SBE I, SBE IIa, SBE Iib, SBE-I, SBE1, SBEI, SBEII, SBEIIA, SBEIIB, ScoBE, SmGBE, starch branching enzyme, starch branching enzyme I, starch branching enzyme II, starch branching enzyme IIa, starch branching enzyme IIb, starch branching enzyme SBE I, starch branching enzyme SBE IIb, starch branching enzyme-I, starch-branching enzyme, starch-branching enzyme Ia, starch-branching enzyme IIa, starch-branching enzyme IIb, starch-branching enzymes IIa, starch-branching enzymes IIb, starch-branching-enzyme, TK1436, VsbeII

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.18 1,4-alpha-glucan branching enzyme

Temperature Stability

Temperature Stability on EC 2.4.1.18 - 1,4-alpha-glucan branching enzyme

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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0 - 45
the enzyme retains about 80% of its activity at 0-45° for 1 h
10 - 30
pre-incubation of the enzyme for 2 h at different temperatures, following enzyme assay is conducted at 30°C. About 80% activity is retained after pre-incubation at 37°C and 45°C
10 - 40
purified recombinant enzyme, the enzyme retains about 80% of its activity at 40°C for 30 min, the enzyme displays excellent cold adaptation over a low temperature range of 10-30°C, retaining over 85% of its relative activity within this range
5 - 45
-
no apparent inactivation up to 30°C, about 10% loss of activity at 35°C, about 90% loss of enzyme activity at 50°C
50 - 55
in the absence of substrates the branching enzyme is stable at 50°C for more than 6 h. At 55°C the half-life of the branching enzyme is 50 min.
50 - 60
the wild type enzyme retains 100, about 75 and about 25% activity after 15 min at 50, 60 and 65°C, respectively. The enzyme is inactive after 15 min at 70°C. Its melting temperature is 63°C
51
-
partial inactivation
60
the wild type enzyme shows a half-life of 42.1 min at 60°C and retains less than 20% activity after 2 h at 60°C. Mutant enzyme Q231R shows a half-life of 53.2 min at 60°C and retains about 31% activity after 2 h at 60°C. Mutant enzyme Q231K shows a half-life of 53.6 min at 60°C and retains about 33% activity after 2 h at 60°C. Mutant enzyme T339E shows a half-life of 49.1 min at 60°C and retains about 28% activity after 2 h at 60°C. Mutant enzyme T339D shows a half-life of 50.4 min at 60°C and retains about 23% activity after 2 h at 60°C. Mutant enzyme I571D shows a half-life of 64.2 min at 60°C and retains about 36% activity after 2 h at 60°C
60 - 65
after 1 h incubation at 60°C fully active, after 1 h incubation at 65°C only 80% residual activity
60 - 70
the half-life of the enzyme at 60 and 70°C is 24 h and 5 h, respectively
70
-
pH 7.0, 30 min, 10% loss of the soluble enzyme form, 50% loss of the insoluble enzyme form
70 - 90
-
the enzyme remains stable at temperatures up to 80°C and has a half-life at 85°C of approximately 31 min. The enzyme is quite stable (for at least 2 h) at 70°C or lower, and 92% of the initial enzyme activity is retained at 80°C for 2 h. Furthermore, the enzyme demonstrates half-lives at 85 and 90°C of 31 min and 2.5 min, respectively
75
-
fully active after 100 min incubation, half-life time at 80°C 73.7 min, half-life time at 85°C 16.7 min
85
-
soluble and insoluble enzyme form, stable up to
90
the protein is stable up to 90°C, the activity decreases gradually during the incubation at 100°C
additional information