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2.4.1.19: cyclomaltodextrin glucanotransferase

This is an abbreviated version!
For detailed information about cyclomaltodextrin glucanotransferase, go to the full flat file.

Word Map on EC 2.4.1.19

Reaction

alpha-D-glucopyranosyl-(1-4)-alpha-D-glucopyranosyl-(1-4)-alpha-D-glucopyranosyl-(1-4)-alpha-D-glucopyranosyl-(1-4)-alpha-D-glucopyranosyl-(1-4)-alpha-D-glucopyranosyl-(1-4)-alpha-D-glucopyranose
=
alpha-cyclodextrin
 +
alpha-D-glucose

Synonyms

1,4-alpha-D-glucopyranosyl transferase, Akrilex C cyclodextrin glycosyltransferase, alpha-1,4-glucan 4-glycosyltransferase, cyclizing, alpha-cgt, alpha-CGTase, alpha-cyclodextrin glucanotransferase, alpha-cyclodextrin glucosyltransferase, alpha-cyclodextrin glycosyltransferase, Bacillus macerans amylase, beta-CGTase, beta-cyclodextrin glucanotransferase, beta-cyclodextrin glucosyltransferase, beta-cyclodextrin glycosyltransferase, beta-cyclodextrinase, BMA, C-CGTase, CD glucanotransferase, CGT, CGT13, CGTase, cgtS, CGTse ET1, CGT_TK, cyclodextrin beta-glucanotransferase, cyclodextrin glucanotransferase, cyclodextrin glucosyltransferase, cyclodextrin glycosyl transferase, cyclodextrin glycosyltransferase, cyclodextrin-glycosyltransferase, cyclodextrinase, cyclomaltodextrin glucanyltransferase, cyclomaltodextrin glucotransferase, cyclomaltodextrin glycanotransferase, cyclomaltodextrin glycosyltransferase, gamma-CGTase, gamma-cyclodextrin glycosyltransferase, konchizaimu, M-CGTase, More, neutral-cyclodextrin glycosyltransferase, PFCGT, Toruzyme

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.19 cyclomaltodextrin glucanotransferase

Cloned

Cloned on EC 2.4.1.19 - cyclomaltodextrin glucanotransferase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
ATCC21783 expressed in Bacillus subtilis
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cgtA gene cloned and expressed in Escherichia coli JM109
cloned and expressed in Escherichia coli
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cloned and overexpressed in Bacillus subtilis
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cloned and overexpressed in Escherichia coli
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coexpression of folding accessory proteins for production of active cyclodextrin glycosyltransferase in Escherichia coli. The optimal coexpression partner, human peptidyl-prolyl cis-trans isomerase, is applied to fed-batch cultures of recombinant Escherichia coli in an attempt to develop an industrialy viable process
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DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain JM109
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DNA and amino acid sequence determination and analysis, phylogenetic tree
expressed as inclusion body in recombinant Escherichia coli. The refolding yield of CGTase is enhanced by 71% by the immobilized poly-arginine fused minichaperone (MiniELR10) compared with a conventional refolding method. Minichaperone immobilized by poly-arginine fusion could assist refolding of heterologous proteins expressed as inclusion body
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expressed in Bacillus subtilis strain WB600
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expressed in Escherichia coli
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli JM109 cells
expressed in in Bacillus subtilis strain WB600
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expressed in Komagataella phaffii
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expression in Bacillus subtilis KN2
-
expression in Escherichia coli
expression in Escherichia coli strain BL21(DE3), glycine, as a medium supplement, can enhance the extracellular secretion of recombinant alpha-CGTase by 11fold. Glycine supplementation exerts impaired cell growth inhibiting an increased enzyme production, but Ca2+ can remedy cell growth inhibition induced by glycine, thereby leading to further increase in the glycine-enhanced extracellular secretion of recombinant alpha-CGTase, effects on the bacterial cell membrane permeability, overview
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expression in Saccharomyces cerevisiae strain EBY100, subcloning in Escherichia coli strain DH5 alpha
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expression of cyclodextrin glycosyltransferase gene fused with thioredoxin, hexa-histidine and S-protein at the N-terminus and a proline-rich peptide at the C-terminus, in Escherichia coli
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expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
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expression of the enzyme in Escherichia coli strain DH5alpha
expression of wild-type and mutant enzymes in Bacillus subtilis strain DB104A
expression of wild-type and mutant enzymes in Bacillus subtilis strain DB104A, subcloning in Escherichia coli strain MC1061
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gene cgt, expression in Escherichia coli strain JM109 and K12 N3406, enzyme secretion, batch fermentation method optimization via medium improvement, overview
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gene cgtase, DNA and amino acid sequence determination and analysis, expression in Escherichia coli periplasmic fraction in different strains at low amounts, overview
gene cloned in Escherichia coli
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gene coding for I transferred to a Bacillus host
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gene pfcgt, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression in Escherichia coli
gene sequencing of 16S rRNA
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isolation of alkaliphilic Bacillus strains and determination of their phylogenetic and phenotypic characteristics, overview
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M5a1 cloned and expressed
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mutant enzyme Y93F is expressed in Escherichia coli BL21(DE3) cells
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mutant enzymes H59Q, Y96M, 90-PPI-92, and DELTA(154–160) are constructed and produced using a recombinant Escherichia coli with a secretive expression system extracellularly
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mutant enzymes I631T, I641T and K647E are expressed in Escherichia coli BL21(DE3) cells
mutant Y167H is expressed in Escherichia coli BL21(DE3) cells
mutant Y195I is expressed in Escherichia coli BL21(DE3) cells
-
no. 38-2 cloned and sequenced
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no. 8 cloned, sequenced and expressed in Escherichia coli
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nucleotide sequence determined
overexpression and extracellular secretion of the recombinant alpha-cyclodextrin glycosyltransferase in Escherichia coli strain BL21(DE3), promotable by glycine, when supplemented optimally at the middle of the exponential growth phase at 1%, the enzyme production is increased by 11fold, glycine supplementation at the beginning of cell growth achieves a 1.2fold activation, overview. Glycine increases the cell membrane permeability, mechanism, overview
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pET-8c vector, gene cloned and expressed in Escherichia coli BL21(DE3)
phylogenetic analysis
phylogenetic analysis, expression in Bacillus subtilis strain DB104A
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phylogenetic analysis, expression in Bacillus subtilis strain NA-1
phylogenetic analysis, expression in Escherichia coli strain BL21(DE3)
phylogenetic analysis, expression of the enzyme from strain BC8 in Escherichia coli strain JM103, and of the enzyme from strain BC251 in Bacillus subtilis strain DB104A
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phylogenetic tree
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sp. 17-1, nucleotide sequence determined
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sp. B1018, nucleotide sequence determined
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strain 1011 cloned in Escherichia coli bacteriophage lambdaD69 and recloned in Escherichia coli plasmid pBR322
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strain 1011 gene sequenced
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strain IFO3490, vector plasmid pTB523, gene cgtM cloned in Bacillus subtilis NA-1, sequence determined, chimeric GTAse gene constructed by using cgt-1 from Bacillus stearothermophilus NO2
strain NO2, mutant CGTases constructed from cgt1 gene and expressed in Bacillus subtilis NA-1
-
strain NO2, vector plasmid pTB523, 3genes, cgt-1, cgt-5 and cgt-232 cloned in Bacillus subtilis NA-1, sequence determined, chimeric GTAse gene constructed by using cgt-1 and cgtM from Bacillus macerans IFO3490
subcloned on pUB140, expressed in Bacillus subtilis LKS88
-
subcloning in Escherichia coli strain JM109, expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
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the cyclodextrin glucanotransferase gene is cloned into plasmid pYD1, which allows regulated expression, secretion, and detection. The expression of CGTase with a-agglutinin at the N-terminal end on the extracellular surface of Saccharomyces cerevisiae is confirmed by immunofluorescence microscopy. This surface-anchored CGTase gives the yeast the ability to directly utilize starch as a sole carbon source and the ability to produce the anticipated products, cyclodextrins, as well as glucose and maltose. The resulting glucose and maltose, which are efficient acceptors in the CGTase coupling reaction, could be consumed by yeast fermentation and thus facilitated cyclodextrin production. On the other hand, ethanol produced by the yeast may form a complex with cyclodextrin and shift the equilibrium in favor of cyclodextrin production. The yeast with immobilized CGTase produces 24.07 mg/ml cyclodextrins when it is incubated in yeast medium supplemented with 4% starch
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