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2.4.1.19: cyclomaltodextrin glucanotransferase

This is an abbreviated version!
For detailed information about cyclomaltodextrin glucanotransferase, go to the full flat file.

Word Map on EC 2.4.1.19

Reaction

alpha-D-glucopyranosyl-(1-4)-alpha-D-glucopyranosyl-(1-4)-alpha-D-glucopyranosyl-(1-4)-alpha-D-glucopyranosyl-(1-4)-alpha-D-glucopyranosyl-(1-4)-alpha-D-glucopyranosyl-(1-4)-alpha-D-glucopyranose
=
alpha-cyclodextrin
+
alpha-D-glucose

Synonyms

1,4-alpha-D-glucopyranosyl transferase, Akrilex C cyclodextrin glycosyltransferase, alpha-1,4-glucan 4-glycosyltransferase, cyclizing, alpha-cgt, alpha-CGTase, alpha-cyclodextrin glucanotransferase, alpha-cyclodextrin glucosyltransferase, alpha-cyclodextrin glycosyltransferase, Bacillus macerans amylase, beta-CGTase, beta-cyclodextrin glucanotransferase, beta-cyclodextrin glucosyltransferase, beta-cyclodextrin glycosyltransferase, beta-cyclodextrinase, BMA, C-CGTase, CD glucanotransferase, CGT, CGT13, CGTase, cgtS, CGTse ET1, CGT_TK, cyclodextrin beta-glucanotransferase, cyclodextrin glucanotransferase, cyclodextrin glucosyltransferase, cyclodextrin glycosyl transferase, cyclodextrin glycosyltransferase, cyclodextrin-glycosyltransferase, cyclodextrinase, cyclomaltodextrin glucanyltransferase, cyclomaltodextrin glucotransferase, cyclomaltodextrin glycanotransferase, cyclomaltodextrin glycosyltransferase, gamma-CGTase, gamma-cyclodextrin glycosyltransferase, konchizaimu, M-CGTase, More, neutral-cyclodextrin glycosyltransferase, PFCGT, Toruzyme

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.19 cyclomaltodextrin glucanotransferase

Purification

Purification on EC 2.4.1.19 - cyclomaltodextrin glucanotransferase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
acetone precipitation, DEAE-cellulose column chromatography, and Sephadex G-100 gel filtration
-
ammonium sulfate precipitation
-
ammonium sulfate precipitation and DEAE-cellulose column chromatography
-
ammonium sulfate precipitation and Ni-NTA agarose column chromatography
-
ammonium sulfate precipitation, DEAE-Sepharose column chromatography and Sephadex G-100 gel filtration
-
beta-cyclodextrin affinity column chromatography
DEAE-Sepharose column chromatography
DF9, 2 different types, smooth and rough variation
-
ethanol precipitation, Superdex 200 gel filtration and DEAE-Sepharose column chromatography
-
from strain H69-3
-
from strain P4
-
from strain ST-12 K
-
from strains 7B, NCIM 5119 and no. 37
-
from strains BT01 and RB01
-
from the alkalophilic strain 20RF
-
from the alkalophilic strain 8SB
-
from the alkalophilic strains 8SB and 20RF
-
His-tag affinity column chromatography
HisTrap column chromatography
-
HisTrap column chromatography, and gel filtration
-
HiTrap Q column chromatography
-
mutant Y167H with ammonium sulfate precipitation and Ni-NTA agarose column chromatography
native CGTase 22.9fold to homogeneity by hydrophobic interaction chromatography and starch adsorption
native enzyme 11.4fold from strain BL-12 by ultrafiltration and beta-cyclodextrin polymer affinity chromatography
-
native enzyme 13.7fold from strain ATCC 21783 by ultrafiltration and consecutive starch adsorption
native enzyme 315fold by ammonium sulfate fractionation, beta-cyclodextrin affinity chromatography, and ultrafiltration
-
native enzyme 47fold by affinity chromatography
-
native enzyme 99fold and recombinant enzyme 7fold from Escherichia coli both by starch adsorption, and hydrophobic interaction or beta-cyclodextrin affinity chromatography
-
native enzyme about 200fold from strain H69-3 by gel filtration, and one or two different steps of anion exchange chromatography
-
native enzyme by adsorption on starch, ion exchange chromatography, and gel filtration
-
native enzyme by starch adsorption and anion exchange chromatography
-
native extracellular enzyme 23.1fold from an alkalophilic strain by ultrafiltration and anion exchange chromatography to homogeneity
-
natural and recombinant enzyme
Ni-NTA agarose column chromatography
Ni-NTA agarose column chromatography and Sephacryl-100 gel filtration
-
Ni-NTA resin column chromatography
-
Ni-Sepharose 6 column chromatography
-
partial purification using a PD-10 desalting column
-
purification and concentration of CGTase by starch adsorption
-
rapid affinity purification
-
recombinant
recombinant enzyme
recombinant enzyme 25fold from Escherichia coli, by heat treatment at 60°C, hydrophobic interaction chromatography and starch adsorption, overview
recombinant enzyme from Escherichia coli strain BL21(DE3) inclusion bodies
-
recombinant enzyme from Escherichia coli strain DH5alpha
recombinant enzyme, cgtM gene product
recombinant enzymes, partially
-
recombinant fusion protein
-
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21 (DE3) by nickel affinity chromatography
-
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
-
Sepharose 4B column chromatography
-
strain 1011, wild-type and mutants
-
wild-type and mutant enzymes
-
wild-type and recombinant enzymes
-
wild-type enzyme and mutant enzymes H59Q, Y96M, 90-PPI-92, and DELTA(154–160) expressed in Escherichia coli
-