2.4.1.19: cyclomaltodextrin glucanotransferase

This is an abbreviated version!
For detailed information about cyclomaltodextrin glucanotransferase, go to the full flat file.

Reaction

Synonyms

1,4-alpha-D-glucopyranosyl transferase, Akrilex C cyclodextrin glycosyltransferase, alpha-1,4-glucan 4-glycosyltransferase, cyclizing, alpha-cgt, alpha-CGTase, alpha-cyclodextrin glucanotransferase, alpha-cyclodextrin glucosyltransferase, alpha-cyclodextrin glycosyltransferase, Bacillus macerans amylase, beta-CGTase, beta-cyclodextrin glucanotransferase, beta-cyclodextrin glucosyltransferase, beta-cyclodextrin glycosyltransferase, beta-cyclodextrinase, BMA, C-CGTase, CD glucanotransferase, CGT, CGT13, CGTase, cgtS, CGTse ET1, CGT_TK, cyclodextrin beta-glucanotransferase, cyclodextrin glucanotransferase, cyclodextrin glucosyltransferase, cyclodextrin glycosyl transferase, cyclodextrin glycosyltransferase, cyclodextrin-glycosyltransferase, cyclodextrinase, cyclomaltodextrin glucanyltransferase, cyclomaltodextrin glucotransferase, cyclomaltodextrin glycanotransferase, cyclomaltodextrin glycosyltransferase, gamma-CGTase, gamma-cyclodextrin glycosyltransferase, konchizaimu, M-CGTase, More, neutral-cyclodextrin glycosyltransferase, PFCGT, Toruzyme

ECTree

     2 Transferases

         2.4 Glycosyltransferases

             2.4.1 Hexosyltransferases

                2.4.1.19 cyclomaltodextrin glucanotransferase

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Results	in table
165	Activating Compound
217	AA Sequence
308	Application
1	CAS Registry Number
117	Cloned(Commentary)
16	Crystallization (Commentary)
3	Expression
3	General Information
23	General Stability
6	IC50 Value
175	Inhibitors
7	kcat/KM [mM/s]
17	Ki Value [mM]
147	KM Value [mM]
78	Localization
117	Metals/Ions
122	Molecular Weight [Da]
103	Natural Substrates/ Products (Substrates)
31	Organic Solvent Stability
949	Organism
6	Pathway
68	PDB ID
211	pH Optimum
50	pH Range
73	pH Stability
2	pI Value
248	Protein Variants
134	Purification (Commentary)
3	Reaction
5	Reaction Type
558	Reference
1	Renatured (Commentary)
23	Source Tissue
100	Specific Activity [micromol/min/mg]
3	Storage Stability
1116	Substrates and Products (Substrate)
152	Subunits
482	Synonyms
1	Systematic Name
241	Temperature Optimum [°C]
46	Temperature Range [°C]
137	Temperature Stability [°C]
127	Turnover Number [1/s]

pH Stability

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of the Enzyme Summary Page.

pH Stability on EC 2.4.1.19 - cyclomaltodextrin glucanotransferase

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pH STABILITY

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

10 - 11

Niallia circulans

-

ATCC 21783, stability decreases by immobilization

488831

4 - 10

2 entries

4.5 - 11

Paenibacillus campinasensis

-

24 h, 25°C, stable

671395

5

Lysinibacillus sphaericus

-

purified native enzyme, 50°C, 4 h, 34.2% retaining activity

702110

5 - 10

Niallia circulans

-

ATCC 21783

488826

5 - 10.5

Weizmannia coagulans

-

activity is not lost by incubation at 4°C for 1 month

488839

5 - 11

3 entries

5 - 7

Bacillus sp. N-227

Q197W1

the enzyme is stable for at least 60 min across a wide pH range of 5.0-7.0. The enzyme becomes unstable at higher pH as indicated by the residual activity dropping from 85% at pH 8.0 to less than 30% at pH 10.0

755664

5 - 8

2 entries

5.5

Paenibacillus macerans

-

the half-life at pH 5.5 is 29.8 h

735493

5.5 - 10

Bacillus sp. (in: Bacteria)

-

INMIA A/7

488855

5.5 - 11

Bacillus sp. (in: Bacteria)

-

strain 1011, wild-type and mutant enzyme, stable at 37°C for 30 min

488851

5.5 - 8.5

Thermoactinomyces vulgaris

-

highly stable

488861

5.5 - 9

5 entries

5.5 - 9.5

4 entries

6 - 10

8 entries

6 - 10.5

Priestia megaterium

-

stable

684523

6 - 11

Bacillus sp. (in: Bacteria)

-

HA3-3-2/ATCC 39612

488832

6 - 11.5

Paenibacillus campinasensis

-

purified enzyme, stable

684517

6 - 7

Lysinibacillus sphaericus

-

purified native enzyme, 50°C, 4 h, almost completely stable

702110

6 - 8

4 entries

6 - 9

5 entries

6 - 9.5

4 entries

6.5 - 8.5

Paenibacillus macerans

-

stable at 50°C

488839

6.5 - 9.5

Bacillus ohbensis

-

stable at 55°C

488839

7 - 10

4 entries

7 - 11

Cytobacillus firmus

-

-

488856

7 - 12

Bacillus sp. (in: Bacteria)

-

37°C, 30 min, stable

657629

7 - 8

Niallia circulans

-

strain E 192, maximum stability, only 14% decrease in activity at pH 7.0

488842

7 - 9

2 entries

7 - 9.2

Geobacillus stearothermophilus

-

stable at 50°C

488839

8

2 entries

8 - 10

2 entries

additional information

3 entries

4 - 10

Alkalihalobacillus lehensis

-

after 1 h of incubation, the enzyme shows less than 20% activity at pH 4.0 and 5.0, about 35% at pH 6.0, 100% at pH 7.0, about 80% at pH 8.0, about 30% at pH 9.0, and about 20% activity at pH 10.0

735782

4 - 10

Paenibacillus macerans

-

-

488830

5 - 11

Bacillus sp. Y112

-

the enzyme retains 100% of its initial activity in a range of pH 7.0 to 9.0 after pre-incubation in buffer for 1 h at 25°C. The enzyme is highly stable at pH 5.0 and 11.0 as indicated by the maintenance of 77% and 72% residual activity, respectively

757110

5 - 11

Niallia circulans

P30920

75% of maximal activity within this range

684450

5 - 11

Salipaludibacillus agaradhaerens

-

stable within

719615

5 - 8

Bacillus sp. (in: Bacteria)

-

quite stable at 40°C for 3 h

488836

5 - 8

Geobacillus stearothermophilus

P31797

the enzyme remains stable for 120 min at pH 5.0-8.0

755883

5.5 - 9

Bacillus autolyticus

-

stable for 12 h at 40°C, below 40°C stable for 1 h at pH 6.0

488849

5.5 - 9

Geobacillus stearothermophilus

-

-

488849

5.5 - 9

Geobacillus stearothermophilus

-

strain B-4006, highly stable

488861

5.5 - 9

Niallia circulans

-

-

488849, 488861

5.5 - 9

Niallia circulans

-

C31

488835

5.5 - 9.5

Bacillus licheniformis

-

strain BIO-9m, highly stable

488861

5.5 - 9.5

Parageobacillus thermoglucosidasius

-

after incubation at various pH values within a wide range (pH 5.5-9.5) for 2 h, the enzyme shows higher than 90% residual activity

758476

5.5 - 9.5

Weizmannia coagulans

-

-

488861

5.5 - 9.5

Weizmannia coagulans

-

stable at 70°C

488839

6 - 10

Alkalihalobacillus alcalophilus

-

strain B-3103, highly stable

488861

6 - 10

Alkalihalobacillus pseudalcaliphilus

-

the beta-cyclodextrin forming activity decreases with decreasing pH from 6.0 to 4.0, and increases with increasing pH from 10.0 to 11.0. However, there is no significant difference in the beta-cyclodextrin forming activity after 1 and 2 h of treatment at pH 6.0-10.0. With regard to gamma-cyclodextrin production, the enzyme activity increases with increasing pH from 4.0 to 8.0, and then decreases to 77.2% and 66.4% after 1 and 2 h at pH 11.0, respectively

757274

6 - 10

Bacillus sp. (in: Bacteria)

-

stable

658790

6 - 10

Niallia circulans

-

-

488861, 488864

6 - 10

Niallia circulans

-

stable at 65°C

488839

6 - 10

Paenibacillus illinoisensis

-

-

488864

6 - 10

Weizmannia coagulans

-

-

488839

6 - 10

Weizmannia coagulans

-

strain BIO-13m, highly stable

488861

6 - 8

Bacillus subtilis

-

stable at 50°C

488839

6 - 8

Niallia circulans

-

-

488839

6 - 8

Niallia circulans

-

ATCC 21783, immobilized enzyme, stable

488831

6 - 8

Paenibacillus sp. xw-6-66

W8CJT2

the enzyme retains more than 80% activity at pH 6.0-8.0 for 30 min

758336

6 - 9

Niallia circulans

-

-

488861

6 - 9

Niallia circulans

-

stable at 70°C

488839

6 - 9

Niallia circulans

-

strain E 192, only 14% decrease in activity

488842

6 - 9

Paenibacillus illinoisensis

-

the enzyme is stable from pH 6.0 to 9.0 for 30 min with a gradual loss of activity at higher and lower pH values in the range of 6.0-9.0

737302

6 - 9

Salimicrobium halophilum

-

strain BIO-12H, highly stable

488861

6 - 9.5

Niallia circulans

-

strain BIO-3m, highly stable

488861

6 - 9.5

Niallia circulans

-

stable at 55°C

488839

6 - 9.5

Paenibacillus macerans

-

-

719222

6 - 9.5

Salimicrobium halophilum

-

strain BIO-13H, highly stable

488861

7 - 10

Alkalihalobacillus alcalophilus

-

strain BA-4229, highly stable

488861

7 - 10

Bacillus sp. (in: Bacteria)

-

-

488839

7 - 10

Bacillus sp. (in: Bacteria)

-

No. 5 strain

488825

7 - 10

Priestia megaterium

-

stable at 65°C

488839

7 - 9

Bacillus sp. (in: Bacteria)

-

60°C, 30 min, stable

660343

7 - 9

Bacillus sp. (in: Bacteria)

Q5U9W0

60°C, 30 min, stable

673391

8

Cytobacillus firmus

-

the purified enzyme is maximally stable at pH 8.0

689822

8

Niallia circulans

-

ATCC 21783, stable for about 2 weeks at 60°C

488831

8 - 10

Lysinibacillus sphaericus

-

purified native enzyme, 50°C, 4 h, 16.8% retaining activity

702110

8 - 10

Paenibacillus macerans

-

-

488825, 488855

additional information

Paenibacillus macerans

-

the immobilized and the crosslinked imprinted CGTase shows higher stability in the ranges from pH 3 to 7 and from pH 9 to 11

673578

additional information

Paenibacillus sp.

-

the immobilized and the crosslinked imprinted CGTase are more stable than the native enzyme in the pH ranges from 3 to 6 and 8 to 11

673578

additional information

Paenibacillus sp.

-

the recombinant enzyme is more stable at higher temperature and lower pH

687470