2.4.1.197: high-mannose-oligosaccharide beta-1,4-N-acetylglucosaminyltransferase

This is an abbreviated version!
For detailed information about high-mannose-oligosaccharide beta-1,4-N-acetylglucosaminyltransferase, go to the full flat file.

Reaction

O-mannopyranosyl-alpha-1,3-[O-mannopyranosyl-alpha-1,3-(O-mannopyranosyl-alpha-1,6)-O-mannopyranosyl-alpha-1,6]-O-mannopyranosyl-beta-1,4-N-acetyl-D-glucosamine

O-mannopyranosyl-alpha-1,3-O-mannopyranosyl-alpha-1,3-(O-mannopyranosyl-alpha-1,6)-[N-acetylglucosamine-beta-1,4-O-mannopyranosyl-alpha-1,6]-O-mannopyranosyl-beta-1,4-N-acetyl-D-glucosamine

Synonyms

acetylglucosamine-oligosaccharide acetylglucosaminyltransferase, acetylglucosaminyltransferase, uridine diphosphoacetylglucosamine-oligosaccharide, UDP-GlcNAc:oligosaccharide beta-N-acetylglucosaminyltransferase

ECTree

2 Transferases

2.4 Glycosyltransferases

2.4.1 Hexosyltransferases

2.4.1.197 high-mannose-oligosaccharide beta-1,4-N-acetylglucosaminyltransferase

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1	CAS Registry Number
4	Inhibitors
7	KM Value [mM]
3	Localization
2	Metals/Ions
2	Organism
1	pH Optimum
1	pH Range
1	Purification (Commentary)
1	Reaction
1	Reaction Type
2	Reference
3	Specific Activity [micromol/min/mg]
7	Substrates and Products (Substrate)
3	Synonyms
1	Systematic Name
2	Temperature Optimum [°C]
1	Temperature Stability [°C]

Substrates Products

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Substrates Products on EC 2.4.1.197 - high-mannose-oligosaccharide beta-1,4-N-acetylglucosaminyltransferase

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UDP-N-acetyl-D-glucosamine + (O-mannopyranosyl-alpha-1,3)-mannopyranosyl-beta-1,4-N-acetyl-glucosamine

UDP + O-mannopyranosyl-alpha-1,3-[N-acetyl-glucosamine-beta-1,4]-mannopyranosyl-beta-1,4-N-acetyl-D-glucosamine

Dictyostelium discoideum

i.e. Man2GlcNAc, very low activity

488873

UDP-N-acetyl-D-glucosamine + methyl O-alpha-mannopyranosyl-1,6-[O-alpha-D-mannopyranosyl-1,3]-alpha-D-mannopyranoside

UDP + methyl O-alpha-mannopyranosyl-1,6-[O-alpha-D-mannopyranosyl-1,3]-alpha-D-mannopyranosyl-1,4-N-acetyl-D-glucosamine

2 entries

UDP-N-acetyl-D-glucosamine + O-mannopyranosyl-alpha-1,2-O-mannopyranosyl-alpha-1,2-O-mannopyranosyl-alpha-1,3-[O-mannopyranosyl-alpha-1,2-O-mannopyranosyl-alpha-1,3-(O-mannopyranosyl-alpha-1,2-O-mannopyranosyl-alpha-1,6)-O-mannopyranosyl-alpha-1,6]-O-mannopyranosyl-beta-1,4-N-acetylglucosamine

UDP + O-mannopyranosyl-alpha-1,2-O-mannopyranosyl-alpha-1,2-O-mannopyranosyl-alpha-1,3-[O-mannopyranosyl-alpha-1,2-O-mannopyranosyl-alpha-1,3-(O-mannopyranosyl-alpha-1,2-O-mannopyranosyl-alpha-1,6)-(N-acetyl-D-glucosamine-beta-1,4)-O-mannopyranosyl-alpha-1,6]-O-mannopyranosyl-beta-1,4-N-acetyl-D-glucosamine

Dictyostelium discoideum

i.e. Man9GlcNAc oligosaccharide, enzyme catalyzes the transfer of N-acetyl-glucosamine to both alpha- and beta linked mannoses

488873

i.e. Man9GlcNAc oligosaccharide with an additional N-acetyl-D-glucosamine residue attached beta-1,4 to the mannose linked alpha-1,6 to the core mannose in an intersecting position

488873

UDP-N-acetyl-D-glucosamine + O-mannopyranosyl-alpha-1,3-mannopyranosyl-alpha-methyl

Dictyostelium discoideum

i.e. Man2Me

488873

UDP-N-acetyl-D-glucosamine + O-mannopyranosyl-alpha-1,3-[O-mannopyranosyl-alpha-1,3-(O-mannopyranosyl-alpha-1,6)-O-mannopyranosyl-alpha-1,6]-O-mannopyranosyl-beta-1,4-N-acetyl-D-glucosamine

UDP + O-mannopyranosyl-alpha-1,3-O-mannopyranosyl-alpha-1,3-(O-mannopyranosyl-alpha-1,6)-[N-acetylglucosamine-beta-1,4-O-mannopyranosyl-alpha-1,6]-O-mannopyranosyl-beta-1,4-N-acetyl-D-glucosamine

Dictyostelium discoideum

i.e. Man5GlcNAc oligosaccharide, 20% of activity compared to Man9GlcNAc

488873

i.e. Man5GlcNAc oligosaccharide with an additional N-acetyl-D-glucosamine residue in an intersected and/or bisected position, so that either the core beta-mannose or the branching alpha-mannose residue accepts the transferred N-acetyl-D-glucosamine residue

488873

UDP-N-acetyl-D-glucosamine + O-mannopyranosyl-alpha-1,3[O-mannopyranosyl-alpha-1,6]-O-mannopyranosyl-beta-1,4-N-acetylglucosamine

UDP + O-mannopyranosyl-alpha-1,3[O-mannopyranosyl-alpha-1,6-(N-acetyl-D-glucosamine beta-1,4)]-O-mannopyranosyl-beta-1,4-N-acetylglucosamine

Dictyostelium discoideum

i.e. Man3GlcNAc

488873