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2.4.1.2: dextrin dextranase

This is an abbreviated version!
For detailed information about dextrin dextranase, go to the full flat file.

Word Map on EC 2.4.1.2

Reaction

[(1->4)-alpha-D-glucosyl]n
+
[(1->6)-alpha-D-glucosyl]m
=
[(1->4)-alpha-D-glucosyl]n-1
 +
[(1->6)-alpha-D-glucosyl]m+1

Synonyms

DDase, dextran dextrinase, dextrin 6-glucosyltransferase, glucosyltransferase, dextrin 6-

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.2 dextrin dextranase

Purification

Purification on EC 2.4.1.2 - dextrin dextranase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
extra- and intracellular enzymes
-
native DDase 187.5fold to homogeneity by anion exchange chromatography and gel filtration
-
one-step centrifugation at 4°C, 20000 g for 20 min
-
phenyl-Toyopearl, gel filtration
-
recombinant N-terminally His-tagged enzyme from Escherichia coli strain BL21(DE3)-CodonPlus-RIL by nickel affinity chromatography, dialysis, and ultrafiltration