2.4.1.20: cellobiose phosphorylase

This is an abbreviated version!
For detailed information about cellobiose phosphorylase, go to the full flat file.

Reaction

Synonyms

Cbp, CbpA, cellobiose phosphorylase A, cellobiose:orthophosphate alpha-D-glucosyltransferase, CPase, CuCbP, GH94 cellobiose phosphorylase, lactose phosphorylase, ra2122, THA_1941, Tm_1848

ECTree

     2 Transferases

         2.4 Glycosyltransferases

             2.4.1 Hexosyltransferases

                2.4.1.20 cellobiose phosphorylase

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Results	in table
1	Activating Compound
146	AA Sequence
8	Application
1	CAS Registry Number
32	Cloned(Commentary)
3	Crystallization (Commentary)
2	Expression
2	General Information
3	General Stability
30	Inhibitors
58	kcat/KM [mM/s]
10	Ki Value [mM]
124	KM Value [mM]
7	Metals/Ions
19	Molecular Weight [Da]
49	Natural Substrates/ Products (Substrates)
6	Organic Solvent Stability
112	Organism
4	Pathway
28	PDB ID
19	pH Optimum
6	pH Range
3	pH Stability
26	Protein Variants
21	Purification (Commentary)
4	Reaction
1	Reaction Type
65	Reference
32	Specific Activity [micromol/min/mg]
205	Substrates and Products (Substrate)
18	Subunits
35	Synonyms
1	Systematic Name
14	Temperature Optimum [°C]
4	Temperature Range [°C]
15	Temperature Stability [°C]
91	Turnover Number [1/s]

KCat KM Value

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KCat KM Value on EC 2.4.1.20 - cellobiose phosphorylase

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kcat/KM VALUE [1/mMs^-1]

SUBSTRATE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

0.29

1,5-anhydro-D-glucitol

Ruminococcus albus

-

pH 6.0, 37°C

719128

0.0198 - 1.43

2-deoxy-D-glucose

5 entries

12.7

6-deoxy-D-glucose

Ruminococcus albus

-

pH 6.0, 37°C

719128

0.0829 - 63.2

alpha-D-glucose 1-phosphate

5 entries

0.266

butyl beta-D-glucoside

Cellulomonas uda

-

mutant N156D/N163D/T508I/E649G/N667A, pH 6.6, 37°C

719136

0.0681 - 47.1

cellobiose

16 entries

5.67

cellopentaose

Thermosipho africanus

B7IED6

at pH 7.5 and 75°C

758413

7.91

cellotetraose

Thermosipho africanus

B7IED6

at pH 7.5 and 75°C

758413

2.98

cellotriose

Thermosipho africanus

B7IED6

at pH 7.5 and 75°C

758413

0.37

D-gentobiose

Ruminococcus albus

-

pH 6.0, 37°C

719128

0.61 - 0.72

D-glucosamine

3 entries

0.031 - 63.2

D-glucose

4 entries

0.0263 - 0.931

D-mannose

5 entries

0.05 - 0.65

D-xylose

2 entries

0.033 - 0.063

ethyl beta-D-glucoside

2 entries

0.00048 - 0.0034

lactose

4 entries

0.006

methyl alpha-D-glucoside

Cellulomonas uda

-

mutant N156D/N163D/T508I/E649G/N667A, pH 6.6, 37°C

719136

0.099 - 0.12

methyl beta-D-glucoside

2 entries

0.0376

N-acetyl-D-glucosamine

Ruminococcus albus

-

mutant enzyme Y648V, at pH 6.0 and 37°C

735893

0.129

phenyl beta-D-glucoside

Cellulomonas uda

-

mutant N156D/N163D/T508I/E649G/N667A, pH 6.6, 37°C

719136

0.0198

2-deoxy-D-glucose

Ruminococcus albus

-

mutant enzyme C485A, at pH 6.0 and 37°C

735893

0.0477

2-deoxy-D-glucose

Ruminococcus albus

-

mutant enzyme Y648V, at pH 6.0 and 37°C

735893

0.357

2-deoxy-D-glucose

Ruminococcus albus

-

wild type enzyme, at pH 6.0 and 37°C

735893

0.36

2-deoxy-D-glucose

Ruminococcus albus

-

pH 6.0, 37°C

719128

1.43

2-deoxy-D-glucose

Ruminococcus albus

-

mutant enzyme Y648F, at pH 6.0 and 37°C

735893

0.0829

alpha-D-glucose 1-phosphate

Ruminococcus albus

-

mutant enzyme Y648V, at pH 6.0 and 37°C

735893

2.09

alpha-D-glucose 1-phosphate

Ruminococcus albus

-

mutant enzyme Y648F, at pH 6.0 and 37°C

735893

4.85

alpha-D-glucose 1-phosphate

Thermosipho africanus

B7IED6

at pH 7.5 and 75°C

758413

8.55

alpha-D-glucose 1-phosphate

Ruminococcus albus

-

mutant enzyme C485A, at pH 6.0 and 37°C

735893

63.2

alpha-D-glucose 1-phosphate

Ruminococcus albus

-

wild type enzyme, at pH 6.0 and 37°C

735893

0.0681

cellobiose

Ruminococcus albus

-

mutant enzyme Y648V, at pH 6.0 and 37°C

735893

0.103

cellobiose

Acetivibrio thermocellus

-

mutant R48R/Q130H/K131Y/K142R/S411G/A423S/V526A/A781K, pH 7.2, 40°C

720434

0.134

cellobiose

Acetivibrio thermocellus

-

mutant Q130H/K131Y/S411G/A423S/A781K, pH 7.2, 40°C

720434

0.189

cellobiose

Acetivibrio thermocellus

-

mutant S411G, pH 7.2, 40°C

720434

0.2

cellobiose

Acetivibrio thermocellus

-

wild-type, pH 7.2, 40°C

720434

0.24

cellobiose

Cellulomonas uda

-

mutant N156D/N163D/T508I/E649G/N667A, pH 6.6, 37°C

719136

0.263

cellobiose

Acetivibrio thermocellus

-

mutant A781 K, pH 7.2, 40°C

720434

0.287

cellobiose

Acetivibrio thermocellus

-

mutant A423S, pH 7.2, 40°C

720434

0.9

cellobiose

Cellulomonas uda

Q7WTR6

pH 6.6, 37°C, recombinant mutant T508A/N667T

706588

0.99

cellobiose

Cellulomonas uda

Q7WTR6

pH 6.6, 37°C, recombinant mutant A397V/T508A/A512T/D557N/N667T/G681S

706588

1.21

cellobiose

Cellulomonas uda

Q7WTR6

pH 6.6, 37°C, recombinant mutant T508I/N667A

706588

1.92

cellobiose

Thermosipho africanus

B7IED6

at pH 7.5 and 75°C

758413

2.36

cellobiose

Ruminococcus albus

-

mutant enzyme Y648F, at pH 6.0 and 37°C

735893

4.56

cellobiose

Cellulomonas uda

Q7WTR6

pH 6.6, 37°C, recombinant wild-type enzyme

706588

5.35

cellobiose

Ruminococcus albus

-

mutant enzyme C485A, at pH 6.0 and 37°C

735893

47.1

cellobiose

Ruminococcus albus

-

wild type enzyme, at pH 6.0 and 37°C

735893

0.61

D-glucosamine

Ruminococcus albus

-

mutant enzyme C485A, at pH 6.0 and 37°C

735893

0.719

D-glucosamine

Ruminococcus albus

-

wild type enzyme, at pH 6.0 and 37°C

735893

0.72

D-glucosamine

Ruminococcus albus

-

pH 6.0, 37°C

719128

0.031

D-glucose

Cellulomonas uda

-

mutant N156D/N163D/T508I/E649G/N667A, pH 6.6, 37°C

719136

0.217

D-glucose

Cellulomonas uda

-

mutant T508I/E649C/N667A, pH 6.6, 37°C

719136

2.12

D-glucose

Thermosipho africanus

B7IED6

at pH 7.5 and 75°C

758413

63.2

D-glucose

Ruminococcus albus

-

pH 6.0, 37°C

719128

0.0263

D-mannose

Ruminococcus albus

-

mutant enzyme Y648V, at pH 6.0 and 37°C

735893

0.0532

D-mannose

Ruminococcus albus

-

mutant enzyme C485A, at pH 6.0 and 37°C

735893

0.112

D-mannose

Ruminococcus albus

-

wild type enzyme, at pH 6.0 and 37°C

735893

0.12

D-mannose

Ruminococcus albus

-

pH 6.0, 37°C

719128

0.931

D-mannose

Ruminococcus albus

-

mutant enzyme Y648F, at pH 6.0 and 37°C

735893

0.05

D-xylose

Thermosipho africanus

B7IED6

at pH 7.5 and 75°C

758413

0.65

D-xylose

Ruminococcus albus

-

pH 6.0, 37°C

719128

0.033

ethyl beta-D-glucoside

Cellulomonas uda

-

mutant T508I/E649C/N667A, pH 6.6, 37°C

719136

0.063

ethyl beta-D-glucoside

Cellulomonas uda

-

mutant N156D/N163D/T508I/E649G/N667A, pH 6.6, 37°C

719136

0.00048

lactose

Cellulomonas uda

Q7WTR6

pH 6.6, 37°C, recombinant wild-type enzyme

706588

0.0015

lactose

Cellulomonas uda

Q7WTR6

pH 6.6, 37°C, recombinant mutant T508I/N667A

706588

0.0021

lactose

Cellulomonas uda

Q7WTR6

pH 6.6, 37°C, recombinant mutant T508A/N667T

706588

0.0034

lactose

Cellulomonas uda

Q7WTR6

pH 6.6, 37°C, recombinant mutant A397V/T508A/A512T/D557N/N667T/G681S

706588

0.099

methyl beta-D-glucoside

Cellulomonas uda

-

mutant N156D/N163D/T508I/E649G/N667A, pH 6.6, 37°C

719136

0.12

methyl beta-D-glucoside

Cellulomonas uda

-

mutant T508I/E649C/N667A, pH 6.6, 37°C

719136