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2.4.1.20: cellobiose phosphorylase

This is an abbreviated version!
For detailed information about cellobiose phosphorylase, go to the full flat file.

Word Map on EC 2.4.1.20

Reaction

cellobiose
+
phosphate
=
alpha-D-glucose 1-phosphate
+
D-glucose

Synonyms

Cbp, CbpA, cellobiose phosphorylase A, cellobiose:orthophosphate alpha-D-glucosyltransferase, CPase, CuCbP, GH94 cellobiose phosphorylase, lactose phosphorylase, ra2122, THA_1941, Tm_1848

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.20 cellobiose phosphorylase

Purification

Purification on EC 2.4.1.20 - cellobiose phosphorylase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
His-bind resin column chromatography
-
Histrap column chromatography, HiTrap Q column chromatography and Superdex 200 gel filtration
-
Ni-chelating column chromatography and HiTrap Q column chromatography
Ni-chelating column chromatography, and gel filtration
-
Ni-NTA agarose slurry, Superdex 200 column and Mono Q column
-
Ni-NTA column chromatography
-
Ni-NTA resin column chromatography and Mono Q column chromatography
-
recombinant enzyme
-
recombinant enzyme from E. coli
recombinant enzyme with a cellobiose-binding intein tag, development of a simple, low-cost, and scalable protein purification method using a biodegradable regenerated amorphous cellulose, affinity adsorption on regenerated amorphous cellulose followed by intein self-cleavage, method evaluation, overview
-
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli by immobilized metal ion affinity chromatography
use of thiol reagent necessary during purification to maintain activity
-