2.4.1.20: cellobiose phosphorylase
This is an abbreviated version!
For detailed information about cellobiose phosphorylase, go to the full flat file.
Word Map on EC 2.4.1.20
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2.4.1.20
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phosphorolysis
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cellodextrins
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phosphorylases
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cellvibrio
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gilvus
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cellulolytic
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thermocellum
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cellulomonas
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synthesis
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alpha-d-glucose
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laminaribiose
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cellobiose-grown
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cellotriose
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alpha-d-glucose-1-phosphate
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phosphorolyzed
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6-deoxy-d-glucose
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analysis
- 2.4.1.20
-
phosphorolysis
- cellodextrins
- phosphorylases
-
cellvibrio
- gilvus
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cellulolytic
- thermocellum
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cellulomonas
- synthesis
- alpha-d-glucose
- laminaribiose
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cellobiose-grown
- cellotriose
- alpha-d-glucose-1-phosphate
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phosphorolyzed
- 6-deoxy-d-glucose
- analysis
Reaction
Synonyms
Cbp, CbpA, cellobiose phosphorylase A, cellobiose:orthophosphate alpha-D-glucosyltransferase, CPase, CuCbP, GH94 cellobiose phosphorylase, lactose phosphorylase, ra2122, THA_1941, Tm_1848
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Purification
Purification on EC 2.4.1.20 - cellobiose phosphorylase
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Histrap column chromatography, HiTrap Q column chromatography and Superdex 200 gel filtration
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Ni-chelating column chromatography and HiTrap Q column chromatography
Ni-NTA resin column chromatography and Mono Q column chromatography
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recombinant enzyme from E. coli
recombinant enzyme with a cellobiose-binding intein tag, development of a simple, low-cost, and scalable protein purification method using a biodegradable regenerated amorphous cellulose, affinity adsorption on regenerated amorphous cellulose followed by intein self-cleavage, method evaluation, overview
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recombinant His-tagged wild-type and mutant enzymes from Escherichia coli by immobilized metal ion affinity chromatography
use of thiol reagent necessary during purification to maintain activity
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