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11 members of the XTH gene family are cloned from Populus tomentosa, sequence comparisons and phylogenetic analysis and tree, recombinant expression of PtoXTH27 in Pichia pastoris strain GS115, sugar and osmotolerance analysis of yeast transformants
11 members of the XTH gene family are cloned from Populus tomentosa, sequence comparisons and phylogenetic analysis and tree, recombinant expression of PtoXTH34 in Pichia pastoris strain GS115, sugar and osmotolerance analysis of yeast transformants
24 XTH genes, HvXTH1-24, sequence comparisons and phylogenetic analysis, the 24 genes are unevenly distributed on the 7 chromosomes, with 10 of them specifically located on chromosome 7H, genetic organization, sequence comparisons and phylogenetic analysis, overview. Four gene pairs (HvXTH6/7, HvXTH17/18, HvXTH19/20, HvXTH21/22) are detected within 100 kb on chromosome 3H and chromosome 7H, which may be the result of tandem duplication
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3 insect-cell-produced XETs: EXGT, TCH4 and MERI-5
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6 isoforms, AdXET1-6 gene family is cloned, a full-length cDNA, AdXET5, is cloned, sequenced and overexpressed as active XET in Escherichia coli, purified XET is encoded by AdXET6
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AtXTH12, DNA and amino acid sequence determination and analysis, expression in Pichia pastoris strain GS115
AtXTH13, DNA and amino acid sequence determination and analysis, expression in Pichia pastoris strain GS115. AtXTH13 cDNA construct is correctly expressed and posttranslationally modified with variable N-linked glycans on one site
AtXTH17, DNA and amino acid sequence determination and analysis, expression in Pichia pastoris strain GS115
AtXTH18, DNA and amino acid sequence determination and analysis, expression in Pichia pastoris strain GS115
AtXTH19, DNA and amino acid sequence determination and analysis, expression in Pichia pastoris strain GS115
AtXTH24 is expressed in baculovirus-infected insect cells
cloning and expression of TCH4 in Escherichia coli
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cloning and expression of TCH4, Meri-5, EXGT and XTR9 using the baculovirus/Sf9 insect cell system, XTR9 is sequenced
cloning and expression of XTR9 using the baculovirus/Sf9 insect cell system, XTR9 is sequenced
DNA and amino acid sequence determination and analysis, expression in Pichia pastoris resulting in a recombinant enzyme which is more highly mannosylated than the native enzyme without having influence on activity
DNA and amino acid sequence determination and analysis, phylogenetic analysis and sequence alignment
DNA and amino acid seuence determination and analysis, the poplar genome contains 40 XTH genes, overview
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DNA sequence determination and analysis, functional expression in Pichia pastoris
DNA sequence determination and analysis, methanol-induced, functional expression of wild-type and N93S mutant enzymes in Pichia pastoris, the recombinant wild-type enzyme is heterogeneous due to presence of variable N-glycosylation and incomplete cleavage of the alpha-factor secretion signal peptide
expressed in Arabidopsis thaliana
expressed in Escherichia coli strain BL21 (DE3)
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expressed in Pichia pastoris
expressed in Pichia pastoris strain GS115
expression in Pichia pastoris
expression in Solanum lycopersicum cv. Money Maker via transfection by Agrobacterium tumefaciens strain C58C1:pGV2260 under control of promoter CaMV-35S. Expression analysis of SlXTH1 tomato gene by real-time quantitative PCR from apical and basal hypocotyls segments of wild-type and transgenic tomato lines, and extensibility of cell walls from apical and basal hypocotyls segments of wild type and transgenic tomato lines
for expression in Pichia pastoris strain GS115
gene AtXTH18, expression analysis by real-time semiquantitative RT-PCR, overexpression in Arabidopsis thaliana
gene AtXTH19, expression analysis by real-time semiquantitative RT-PCR, overexpression in Arabidopsis thaliana
gene AtXTH20, expression analysis by real-time semi-quantitative RT-PCR, overexpression in Arabidopsis thaliana
gene DcXTH1, DNA and amino acid sequence determination and analysis, genetic structure, sequence comparisons, and quantitative tissse expression analysis by real-time RT-PCR
gene DcXTH2, DNA and amino acid sequence determination and analysis, genetic structure, sequence comparisons, and quantitative tissue expression analysis by real-time RT-PCR
gene DcXTH3, DNA and amino acid sequence determination and analysis, genetic structure, sequence comparisons, and quantitative tissse expression analysis by real-time RT-PCR
gene DkXTH1, DNA and amino acid sequence determination and analysis, sequence comparison of isozymes and phylogenetic analysis, quantitative real-time PCR expression analysis, mature coding region of DkXTH1 is expressed in Escherichia coli strain BL21, recombinant expression of GFP-tagged isozyme lacking the sugnal peptide and of the DkXTH1 signal peptide fused to GFP in onion cells
gene DkXTH2, DNA and amino acid sequence determination and analysis, sequence comparison of isozymes and phylogenetic analysis, quantitative real-time PCR expression analysis, the isozyme is expressed without the N-terminal leader sequence in Escherichia coli strain BL21, recombinant expression of full-length GFP-tagged isozyme in onion cells
gene DkXTH3, DNA and amino acid sequence determination and analysis, phylogenetic analysis, quantitative real-time PCR expression analysis, recombinant expression of His-tagged isozyme in Escherichia coli strain BL21
gene DkXTH4, DNA and amino acid sequence determination and analysis, quantitative real-time PCR expression analysis, sequence comparison of isozymes and phylogenetic analysis, recombinant expression of His-tagged isozyme in Escherichia coli strain BL21
gene DkXTH5, DNA and amino acid sequence determination and analysis, quantitative real-time PCR expression analysis, sequence comparison of isozymes and phylogenetic analysis, recombinant expression of His-tagged isozyme in Escherichia coli strain BL21
gene DXTH1, DNA and amino acid sequence determination and analysis, phylogenetic analysis, quantitative realtime PCR expression analysis. The isozyme encoding DKXTH1 and DKXTH2 genes show different expression patterns during fruit ripening
gene DXTH2, DNA and amino acid sequence determination and analysis, phylogenetic analysis, quantitative realtime PCR expression analysis. The isozyme encoding DKXTH1 and DKXTH2 genes show different expression patterns during fruit ripening
gene HvXET3, DNA and amino acid sequence determination and analysis, expression in Pichia pastoris
gene HvXET4, DNA and amino acid sequence determination and analysis, expression in Pichia pastoris
gene HvXET6, DNA and amino acid sequence determination and analysis, expression in Pichia pastoris
gene LeXET2, DNA and amino acid sequence determination and analysis, phylogenetic analysis, isozyme expression analysis by quantitative real-time PCR
Q40144, Q43527, Q43528, Q6RHX7, Q6RHX8, Q6RHX9, Q6RHY0, Q6RHY1, Q9FR51, Q9FZ05, Q9SLN9
gene MdXTH1, DNA and amino acid sequence determination and analysis, phylogenetic analysis, isozyme expression analysis by quantitative real-time PCR
C0IRH4, C0IRH6, C0IRH7, C0IRH8, C0IRH9, C0IRI0, C0IRI1, C0IRI2, C0IRI3, C0IRI4, Q8GTJ0
gene MdXTH10, DNA and amino acid sequence determination and analysis, phylogenetic analysis, isozyme expression analysis by quantitative real-time PCR
C0IRH4, C0IRH6, C0IRH7, C0IRH8, C0IRH9, C0IRI0, C0IRI1, C0IRI2, C0IRI3, C0IRI4, Q8GTJ0
gene MdXTH11, DNA and amino acid sequence determination and analysis, phylogenetic analysis, isozyme expression analysis by quantitative real-time PCR
C0IRH4, C0IRH6, C0IRH7, C0IRH8, C0IRH9, C0IRI0, C0IRI1, C0IRI2, C0IRI3, C0IRI4, Q8GTJ0
gene MdXTH3, DNA and amino acid sequence determination and analysis, phylogenetic analysis, isozyme expression analysis by quantitative real-time PCR
C0IRH4, C0IRH6, C0IRH7, C0IRH8, C0IRH9, C0IRI0, C0IRI1, C0IRI2, C0IRI3, C0IRI4, Q8GTJ0
gene MdXTH4, DNA and amino acid sequence determination and analysis, phylogenetic analysis, isozyme expression analysis by quantitative real-time PCR
C0IRH4, C0IRH6, C0IRH7, C0IRH8, C0IRH9, C0IRI0, C0IRI1, C0IRI2, C0IRI3, C0IRI4, Q8GTJ0
gene MdXTH5, DNA and amino acid sequence determination and analysis, phylogenetic analysis, isozyme expression analysis by quantitative real-time PCR
C0IRH4, C0IRH6, C0IRH7, C0IRH8, C0IRH9, C0IRI0, C0IRI1, C0IRI2, C0IRI3, C0IRI4, Q8GTJ0
gene MdXTH6, DNA and amino acid sequence determination and analysis, phylogenetic analysis, isozyme expression analysis by quantitative real-time PCR
C0IRH4, C0IRH6, C0IRH7, C0IRH8, C0IRH9, C0IRI0, C0IRI1, C0IRI2, C0IRI3, C0IRI4, Q8GTJ0
gene MdXTH7, DNA and amino acid sequence determination and analysis, phylogenetic analysis, isozyme expression analysis by quantitative real-time PCR
C0IRH4, C0IRH6, C0IRH7, C0IRH8, C0IRH9, C0IRI0, C0IRI1, C0IRI2, C0IRI3, C0IRI4, Q8GTJ0
gene MdXTH8, DNA and amino acid sequence determination and analysis, phylogenetic analysis, isozyme expression analysis by quantitative real-time PCR
C0IRH4, C0IRH6, C0IRH7, C0IRH8, C0IRH9, C0IRI0, C0IRI1, C0IRI2, C0IRI3, C0IRI4, Q8GTJ0
gene MdXTH9, DNA and amino acid sequence determination and analysis, phylogenetic analysis, isozyme expression analysis by quantitative real-time PCR
C0IRH4, C0IRH6, C0IRH7, C0IRH8, C0IRH9, C0IRI0, C0IRI1, C0IRI2, C0IRI3, C0IRI4, Q8GTJ0
gene PrXTH1, DNA and amino acid sequence determination and analyyis, phylogenetic analysis, gene PrXTH1 belongs to group I of XTHs. The recombinant purified protein has only transglycosylase activity. Quantitative real time-PCR enzyme expression analysis. Recombinant expression of C-terminally HIs-tagged enzyme in Pichia pastoris strain X-33
gene SIXTH12, DNA and amino acid sequence determination and analysis, phylogenetic analysis, isozyme expression analysis by quantitative real-time PCR
Q40144, Q43527, Q43528, Q6RHX7, Q6RHX8, Q6RHX9, Q6RHY0, Q6RHY1, Q9FR51, Q9FZ05, Q9SLN9
gene SlXET4, DNA and amino acid sequence determination and analysis, phylogenetic analysis, isozyme expression analysis by quantitative real-time PCR
Q40144, Q43527, Q43528, Q6RHX7, Q6RHX8, Q6RHX9, Q6RHY0, Q6RHY1, Q9FR51, Q9FZ05, Q9SLN9
gene SlXTH1, DNA and amino acid sequence determination and analysis, phylogenetic analysis, isozyme expression analysis by quantitative real-time PCR
Q40144, Q43527, Q43528, Q6RHX7, Q6RHX8, Q6RHX9, Q6RHY0, Q6RHY1, Q9FR51, Q9FZ05, Q9SLN9
gene SlXTH3, DNA and amino acid sequence determination and analysis, phylogenetic analysis, isozyme expression analysis by quantitative real-time PCR
Q40144, Q43527, Q43528, Q6RHX7, Q6RHX8, Q6RHX9, Q6RHY0, Q6RHY1, Q9FR51, Q9FZ05, Q9SLN9
gene SlXTH5, DNA and amino acid sequence determination and analysis, phylogenetic analysis, isozyme expression analysis by quantitative real-time PCR
Q40144, Q43527, Q43528, Q6RHX7, Q6RHX8, Q6RHX9, Q6RHY0, Q6RHY1, Q9FR51, Q9FZ05, Q9SLN9
gene SlXTH6, DNA and amino acid sequence determination and analysis, phylogenetic analysis, isozyme expression analysis by quantitative real-time PCR
Q40144, Q43527, Q43528, Q6RHX7, Q6RHX8, Q6RHX9, Q6RHY0, Q6RHY1, Q9FR51, Q9FZ05, Q9SLN9
gene SlXTH7, DNA and amino acid sequence determination and analysis, phylogenetic analysis, isozyme expression analysis by quantitative real-time PCR
Q40144, Q43527, Q43528, Q6RHX7, Q6RHX8, Q6RHX9, Q6RHY0, Q6RHY1, Q9FR51, Q9FZ05, Q9SLN9
gene SlXTH8, DNA and amino acid sequence determination and analysis, phylogenetic analysis, isozyme expression analysis by quantitative real-time PCR
Q40144, Q43527, Q43528, Q6RHX7, Q6RHX8, Q6RHX9, Q6RHY0, Q6RHY1, Q9FR51, Q9FZ05, Q9SLN9
gene SlXTH9, DNA and amino acid sequence determination and analysis, phylogenetic analysis, isozyme expression analysis by quantitative real-time PCR
Q40144, Q43527, Q43528, Q6RHX7, Q6RHX8, Q6RHX9, Q6RHY0, Q6RHY1, Q9FR51, Q9FZ05, Q9SLN9
gene structure, genomic localization and phylogenetic relationship of the XTH gene family
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gene tXET-B1, DNA and amino acid sequence determination and analysis, phylogenetic analysis, isozyme expression analysis by quantitative real-time PCR
Q40144, Q43527, Q43528, Q6RHX7, Q6RHX8, Q6RHX9, Q6RHY0, Q6RHY1, Q9FR51, Q9FZ05, Q9SLN9
gene tXET-B2, DNA and amino acid sequence determination and analysis, phylogenetic analysis, isozyme expression analysis by quantitative real-time PCR
Q40144, Q43527, Q43528, Q6RHX7, Q6RHX8, Q6RHX9, Q6RHY0, Q6RHY1, Q9FR51, Q9FZ05, Q9SLN9
gene XET02, DNA and amino acid sequence determination and analysis, phylogenetic analysis, isozyme expression analysis by quantitative real-time PCR
C0IRH4, C0IRH6, C0IRH7, C0IRH8, C0IRH9, C0IRI0, C0IRI1, C0IRI2, C0IRI3, C0IRI4, Q8GTJ0
gene XET1, DNA and amino acid sequence determination and analysis, cloning from germinating seeds, sequence comparisons and phylogenetic tree
gene XET16A, expression analysis by real-time semi-quantitative RT-PCR, overexpression in Arabidopsis thaliana
gene xet6.3, sequence comparisons and analysis, recombinant expression of His-/FLAG-tagged wild-type and mutant enzymes in Escherichia coli strain XL10-Gold, recombinant expression of His-/FLAG-tagged wild-type and mutant enzymes in Pichia pastoris strain GS115, from the latter the enzymes are secreted
gene XTH1, DNA and amino acid sequence determination and analysis, phylogenetic analysis, quantitative real-time RT-PCR enzyme expression analysis, constitutive recombinant expression in Nicotiana tabacum plants via transfection with Agrobacterium tumefaciens strain AGL0
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gene XTH1, DNA and amino acid sequence determination and analysis, quantitative real-time RT-PCR enzyme expression analysis
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gene XTH1, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, quantitative real-time (qRT)-PCR expession analysis, recombinant expression of GFP-tagged isozyme in onion epidermal cells, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21
gene XTH1, recombinant expression of His-tagged enzyme in Pichia pastoris
gene XTH10, isolation of genes XTH2 and XTH10 from an SSH library for the Taishanzaoxia apple, recombinant expression in Solanum lycopersicum plants via Agrobacterium tumefaciens strain LBA4404 transfection method
gene XTH15, recombinant expression in Pichia pastoris
gene XTH15, recombinant expression in Pichia pastoris strain X33, the enzyme is secreted
gene XTH2, cloned from young leaves, recombinant expression in Pichia pastoris strain X-33, subcloning in Escherichia coli
gene XTH2, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, quantitative real-time (qRT)-PCR expession analysis, recombinant expression of GFP-tagged isozyme in onion epidermal cells, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21
gene XTH2, isolation of genes XTH2 and XTH10 from an SSH library for the Taishanzaoxia apple, recombinant expression in Solanum lycopersicum plants via Agrobacterium tumefaciens strain LBA4404 transfection method
gene XTH30, recombinant expression of GFP- or YFP-tagged isozyme XTH30 in Arabidopsis thaliana and heterologous expression of wild-type and mutant enzymes in Nicotiana benthamiana leaves via Agrobacterium tumefaciens strain GV3101-mediated transformation, real-time PCR enzyme expression analysis. Expression of a XTH30 promoter::GUS construct in Arabidopsis thaliana
gene XTH31, recombinant expression in Pichia pastoris
gene XTH31, recombinant expression in Pichia pastoris strain X33, the enzyme is secreted
gene XTH6, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, quantitative real-time PCR expession analysis, recombinant expression of GFP-tagged isozyme in onion epidermal cells. Recombinant expression of DkXTH6 without the signal peptide in Escherichia coli strain BL21
gene XTH7, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, quantitative real-time PCR expession analysis, recombinant expression of GFP-tagged isozyme in onion epidermal cells, recombinant expression of DkXTH7 without the signal peptide in Escherichia coli strain BL21
gene XTH8, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, transient expression in onion epidermal cells at the cell wall, recombinant overexpression of DkXTH8 in Arabidopsis thaliana and in Solanum lycopersicum
genes GhXTH1, GhXTH2, and GhXTH3, DNA and amino acid sequence determination and analysis, genetic structure, expression analysis, recombinant overexpression of isozyme GHXTH1 in transgenic cotton plants using Agrobacterium tumefaciens transfection method under transcriptional control of the CaMV 35S promoter
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genes SIXTH13-SlXTH25, DNA and amino acid sequence determination and analysis, phylogenetic analysis, isozyme expression analysis by quantitative real-time PCR
Q40144, Q43527, Q43528, Q6RHX7, Q6RHX8, Q6RHX9, Q6RHY0, Q6RHY1, Q9FR51, Q9FZ05, Q9SLN9
genes XET1 and NXG1 encode 2 enzyme forms, XET1 cDNA is cloned and sequenced, amino acid sequence of NGX1
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identification of several genes designated Ac(F153)XTH1 to Ac(F153)XTH24, sequence comparisons and phylogenetic analysis, genetic structure analysis and pattern of the motif in XTHs, genome-wide analysis, quantitative real-time PCR expression analysis. Detailed overview
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identification of several genes designated Ac(MD2)XTH1 to Ac(MD2)XTH24, sequence comparisons and phylogenetic analysis, genetic structure analysis and pattern of the motif in XTHs, genome-wide analysis, quantitative real-time PCR expression analysis. Detailed overview
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into the pBIm and pCAM-BIA1300 vector, and into the pPIC6A expression vector for the production of recombinant AtXTH21 protein using the Pichia pastoris expression system
into the pET28a+ vector for transformation of Escherichia coli BL21 cells, into the pPICZalphaA vector for transformation of Pichia pastoris X-33 cells, into the pCAMBIA1302 and pCAMBIA1300 vectors for transformation of onion and Arabidopsis thaliana cells, respectively
isoform XTH14 is expressed in Escherichia coli BL21 cells
isoform XTH5 is expressed in Escherichia coli BL21 cells
isoform XTH7 is expressed in Escherichia coli BL21 cells
LeEXT gene encoding XET is cloned
NtXET-1 is cloned and expressed in Escherichia coli, ORF encodes a 295 amino acids protein, cDNA is cloned in sense and antisense orientation, creation of transgenic tobacco plants with reduced NtXET-1 expression: 2 independent lines with reduced total XET activity by 56% and 37%, respectively, in midribs of tobacco plants transformed with an antisense construct, which exhibit an at least 20% increase in the average MW of xyloglucan
phylogenetic tree and expression analysis of XTH genes, transgenic overexpression of PtxtXET16-34 in Populus tremula x tremuloides
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phylogenetic tree of xyloglucan endotransglucosylase/hydrolases (XTHs) from different species, overexpression of FvXTH6 in Fragaria x ananassa cv. Elsanta fruits, coexpression with FvXTH9, recombinant expression in Nicotiana tabacum cv. Samsum Agrobacterium tumefaciens GV3101/pSoup transfection system for localization studies, recombinant expression of His-tagged enzyme in Saccharomyces cerevisiae INVSc1 cells
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phylogenetic tree of xyloglucan endotransglucosylase/hydrolases (XTHs) from different species, overexpression of FvXTH9 in Fragaria x ananassa cv. Elsanta fruits, coexpression with FvXTH6, recombinant expression in Nicotiana tabacum cv. Samsum via Agrobacterium tumefaciens GV3101/pSoup transfection system for localization studies, recombinant expression of His-tagged enzyme in Saccharomyces cerevisiae INVSc1 cells
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PttXET16-34 is cloned and recombinantly expressed in Pichia pastoris
PttXET16A, a XET isoform in secondary vascular tissues and a member of XET subfamily I, is cloned, sequenced and expressed in Escherichia coli BL21(DE3), structure of the coding sequence
recombinant I expression in Pichia pastoris
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single copy gene, cDNA encoding XET is cloned, sequenced and encodes a 33.5 kDa precursor polypeptide, which is subsequently processed to a 31 kDa mature protein, NXG1 and 2 may be different alleles of the XET gene
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small multi-gene family encodes XET: tXETB1, -B2, -B3 and -B4, tXET-B2 is cloned, sequenced and overexpressed in Escherichia coli, nucleotide and amino acid sequence of tXET-B1
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TCH4 is a member of the XET-related gene family
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XTH gene DcXTH4, DNA and amino acid sequence determination and analysis, genetic structure, sequence comparisons, and quantitative tissse expression analysis by real-time RT-PCR
expressed in Arabidopsis thaliana
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expressed in Arabidopsis thaliana
expressed in Pichia pastoris
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expressed in Pichia pastoris
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expressed in Pichia pastoris
expressed in Pichia pastoris
expressed in Pichia pastoris strain GS115
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expressed in Pichia pastoris strain GS115
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expressed in Pichia pastoris strain GS115
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expressed in Pichia pastoris strain GS115
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into the pMD-18T vector
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into the pMD-18T vector
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PttXET16-34 is cloned and recombinantly expressed in Pichia pastoris
PttXET16-34 is cloned and recombinantly expressed in Pichia pastoris
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