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2.4.1.21: starch synthase (glycosyl-transferring)

This is an abbreviated version!
For detailed information about starch synthase (glycosyl-transferring), go to the full flat file.

Word Map on EC 2.4.1.21

Reaction

ADP-alpha-D-glucose
+
[(1->4)-alpha-D-glucosyl]n
=
ADP
 +
[(1->4)-alpha-D-glucosyl]n+1

Synonyms

adenosine diphosphate glucose-starch glucosyltransferase, adenosine diphosphoglucose-starch glucosyltransferase, ADP-glucose starch synthase, ADP-glucose synthase, ADP-glucose transglucosylase, ADP-glucose-starch glucosyltransferase, ADP-glucose:1,4-alpha-D-glucan 4-alpha-D-glucosyltransferase, ADPG starch synthetase, ADPG-starch glucosyltransferase, AtSS2, AtSSII, AtSSIII, CkGBSS, dull1, GBSS, GBSSI, glgA, glucosyltransferase, adenosine diphosphoglucose-starch, glycogen synthase, glycogen/starch synthase, granule bound starch synthase, granule-bound starch synthase, granule-bound starch synthase I, GSase, OsGBSSII, OsSSIII-1, OsSSIII-2, OsSSIV-1, OsSSIV-2, PvSSI, PvSSII-1, SBD123, SS1, SS4, SSI, SSII, SSII(a), SSIIa, SSIIc, SSIII, SSIII(a), SSIII-1, SSIII-B, SSIII-C, SSIIIa, SSIIIb, SSIV, SSS, SSSI, SSV, starch synthase, starch synthase 1, starch synthase 4, starch synthase I, starch synthase II, starch synthase IIa, starch synthase III, starch synthase III-1, starch synthase III-2, starch synthase III-B, starch synthase IIIa, starch synthase IV, starch synthase IV-1, starch synthase IV-2, starch synthetase, starch-synthase III, starch/glycogen synthase, TaSSIVb, TVAG_258220

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.21 starch synthase (glycosyl-transferring)

Engineering

Engineering on EC 2.4.1.21 - starch synthase (glycosyl-transferring)

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
C164S
C164S/C265S
the mutant shows strongly reduced activity compared to the wild type enzyme
C164S/C545S
C209S
the mutant shows wild type activity
C261S
the mutant shows reduced activity compared to the wild type enzyme
C265S
C265S/C545S
C442S
the mutant shows reduced activity compared to the wild type enzyme
C458S
the mutant shows reduced activity compared to the wild type enzyme
C533S
the mutant shows wild type activity
C545S
W366A
-
mutation in starch-binding domains, 3fold decrease in affinity to starch
W366A/Y394A
-
mutation in starch-binding domains, significant decrease in affinity to starch
Y394A
-
mutation in starch-binding domains, 2fold decrease in affinity to starch
C7S/C409S
exhibits comparable specific activity and apparent affinity for ADP-Glc to wild-type. Use as alternative in crystallization trials to avoid aggregation
E377A
C126S
the mutant shows strongly reduced activity compared to the wild type enzyme
C506S
the mutant shows strongly reduced activity compared to the wild type enzyme
F538A
the mutant shows strongly reduced activity compared to the wild type enzyme
E352A
-
the mutation renders the enzyme about 146 and 14fold less active regards ADP-alpha-D-glucose and rabbit muscle glycogen, respectively
F138A
-
the mutant shows reduced activity compared to the wild type enzyme
K275A
-
the mutation renders the enzyme about 327 and 74fold less active regards ADP-alpha-D-glucose and rabbit muscle glycogen, respectively
R270A
-
the mutation renders the enzyme about 301 and 52fold less active regards ADP-alpha-D-glucose and rabbit muscle glycogen, respectively
W122A/Y124A
-
the mutant shows reduced activity compared to the wild type enzyme
W279A
-
the mutant shows reduced activity compared to the wild type enzyme
W304A
-
the mutant shows reduced activity compared to the wild type enzyme
Y147A
-
the mutant shows reduced activity compared to the wild type enzyme
K193E
-
mutation does not alter the binding of ADP-glucose, Km-value for amylopectin is 3.6fold higher than the wild-type value, KM-value for glycogen is 3.2fold lower than the wild-type value
K193Q
-
mutation does not alter the binding of ADP-glucose, Km-value for amylopectin is 1.5fold lower than the wild-type value, KM-value for glycogen is 15.5fold lower than the wild-type value
K193R
-
mutation does not alter the binding of ADP-glucose, Km-value for amylopectin is 2.6fold higher than the wild-type value, KM-value for glycogen is 2.5fold lower than the wild-type value