2.4.1.210: limonoid glucosyltransferase
This is an abbreviated version!
For detailed information about limonoid glucosyltransferase, go to the full flat file.
Reaction
Synonyms
CitLGT-1, CitLGT-2, CrLGT, cuGT, LGT, LGTase, limonoid glucosyltransferase, limonoid GTase, limonoid UDP glucosyltransferase, limonoid UDP-glucosyltransferase, SLLGT, UDP-D-glucose:limonoid glucosyltransferase, UDP-glucose:limonoid glucosyltransferase
ECTree
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Cloned
Cloned on EC 2.4.1.210 - limonoid glucosyltransferase
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cDNA clone encoding limonoid UDP-glucosyltransferase isolated, single copy gene in the citrus genome
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Citrus unshiu is heterozygous posxadsessing both alleles, CitLGT1/CitLGT2, two alleles (CitLGT1/ CitLGT2) of LGT at a single locus
CrLGT cloning of full-length gene from a cDNA library, DNA and amino acid sequence determination and analysis, genetic structure, sequence comparisons and phylogenetic analysis, cloning in Escherichia coli strain DH5alpha, RT-PCR enzyme expression analyxadsis
expression in Escherichia coli for sequencing
gene SLLGT, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression in Escherichia coli and in Pichia pastoris
gene SLLGT, single copy gene encoding LGT
recombinant expression of the MBP-tagged enzyme (MBP-cuGT) in Escherichia coli strain BLR(DE3). MBP-cuGT does not exhibit any detectable effect on limonin degradation when Navel orange juice is used as the substrate. MBP-cuGT is unable to biotransform either limonoate A-ring lactone (LARL) or limonoate as purified substrates. But MBP-cuGT displays a broad activity spectrum towards flavonoids, confirming that the enzyme produced is active under the conditions evaluated in vitro, expression method evaluation, overview
single copy gene encoding LGT
single copy gene encoding LGT, Citrus sinensis is homozygous for allele CitLGT1