2.4.1.211: 1,3-beta-galactosyl-N-acetylhexosamine phosphorylase

This is an abbreviated version!
For detailed information about 1,3-beta-galactosyl-N-acetylhexosamine phosphorylase, go to the full flat file.

Reaction

Synonyms

1,3-beta-galactosyl-N-acetylhexosamine phosphorylase, beta-1,3-galactosyl-N-acetylhexosamine phosphorylase, beta-D-galactopyranosyl-(1-3)-N-acetyl-D-hexosamine:phosphate galactosyltransferase, BLLJ-1623, cphy0577 protein, cphy3030 protein, D-galactosyl-beta1,3-N-acetyl-D-hexosamine phosphorylase, Gal-GlyNAcP, Gal-HexNAcP, galacto-N-biose phosphorylase, galacto-N-biose/lacto-N-biose I phosphorylase, galacto-N-biose/LNB phosphorylase, GalHexNAcP, GL-BP, GLNBP, GNB/LNB phosphorylase, GnpA, lacto-N-biose I phosphorylase, lacto-N-biose phosphorylase, lnba, lnba1, lnba2, LNBP, lnpA, N-biose/lacto-N-biose I-binding protein

ECTree

     2 Transferases

         2.4 Glycosyltransferases

             2.4.1 Hexosyltransferases

                2.4.1.211 1,3-beta-galactosyl-N-acetylhexosamine phosphorylase

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Results	in table
700	AA Sequence
7	Application
1	CAS Registry Number
11	Cloned(Commentary)
3	Crystallization (Commentary)
10	General Information
1	General Stability
20	kcat/KM [mM/s]
39	KM Value [mM]
2	Localization
10	Molecular Weight [Da]
2	Natural Substrates/ Products (Substrates)
44	Organism
22	PDB ID
8	pH Optimum
4	pH Range
6	pH Stability
62	Protein Variants
10	Purification (Commentary)
2	Reaction
1	Reaction Type
37	Reference
29	Specific Activity [micromol/min/mg]
65	Substrates and Products (Substrate)
3	Subunits
83	Synonyms
1	Systematic Name
6	Temperature Optimum [°C]
1	Temperature Range [°C]
7	Temperature Stability [°C]
27	Turnover Number [1/s]

Temperature Stability

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Temperature Stability on EC 2.4.1.211 - 1,3-beta-galactosyl-N-acetylhexosamine phosphorylase

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TEMPERATURE STABILITY

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

20 - 50

Clostridium perfringens ATCC 13124

-

in 100 mM buffer containing 20 micromolar DTT

684635

20 - 60

Bifidobacterium bifidum

-

-

288692

30

Lachnoclostridium phytofermentans

A9KIW5, A9KQ75

protein is thermostable up to 30°C

693235

30 - 55

Vibrio vulnificus

A0A3Q0KZS1

the enzyme is stable at temperatures up to 30°C during incubation for 30 min, the reactions proceed linearly for 5 min at temperatures up to 37°C but do not proceed linearly at 45°C, no reaction is observed at 55°C

701782

37

Cutibacterium acnes

B7XEJ0, B7XEJ1

the enzyme is stable up to 37°C

701840

45

Lachnoclostridium phytofermentans

A9KIW5, A9KQ75

protein is thermostable up to 45°C

693235

55

Bifidobacterium longum subsp. Longum

E8MF13

rapid inactivation of wild-type enzyme, slower inactivation of mutants R209H, G437S, and N506S, mutants C236Y and D576V retain some activity

737186