2.4.1.212: hyaluronan synthase
This is an abbreviated version!
For detailed information about hyaluronan synthase, go to the full flat file.
Word Map on EC 2.4.1.212
-
2.4.1.212
-
glycosaminoglycans
-
collagen
-
polysaccharide
-
keratinocytes
-
cartilage
-
4-methylumbelliferone
-
proteoglycans
-
hyaluronidases
-
chondrocytes
-
dermal
-
mesenchymal
-
pericellular
-
pasteurella
-
udp-glucuronic
-
hyals
-
versican
-
cumulus
-
synovial
-
vocal
-
articular
-
aggrecan
-
osteoarthritis
-
streptococcal
-
udp-n-acetylglucosamine
-
multocida
-
rhamm
-
pyogenes
-
udp-sugars
-
chondroitin
-
glucuronic
-
procollagen
-
synoviocytes
-
cumulus-oocyte
-
decorin
-
udp-glcua
-
photoaging
-
filaggrin
-
equisimilis
-
ha-binding
-
temporomandibular
-
glcua
-
perineuronal
-
ophthalmopathy
-
zooepidemicus
-
uvb-irradiated
-
ha-mediated
-
tnfaip6
-
medicine
-
hyaluronan-binding
-
fibromodulin
-
biglycan
-
analysis
-
synthesis
-
drug development
-
biotechnology
- 2.4.1.212
- glycosaminoglycans
- collagen
- polysaccharide
- keratinocytes
- cartilage
- 4-methylumbelliferone
- proteoglycans
- hyaluronidases
- chondrocytes
-
dermal
- mesenchymal
-
pericellular
- pasteurella
-
udp-glucuronic
-
hyals
- versican
-
cumulus
- synovial
-
vocal
-
articular
- aggrecan
- osteoarthritis
- streptococcal
- udp-n-acetylglucosamine
- multocida
-
rhamm
- pyogenes
- udp-sugars
- chondroitin
-
glucuronic
- procollagen
- synoviocytes
-
cumulus-oocyte
- decorin
- udp-glcua
-
photoaging
- filaggrin
- equisimilis
-
ha-binding
-
temporomandibular
-
glcua
-
perineuronal
- ophthalmopathy
- zooepidemicus
-
uvb-irradiated
-
ha-mediated
-
tnfaip6
- medicine
-
hyaluronan-binding
- fibromodulin
- biglycan
- analysis
- synthesis
- drug development
- biotechnology
Reaction
Synonyms
CHAS2, CHAS3, class I hyaluronan synthase, CPS1, DG42 protein, HA synthase, HA synthase 3, HA1, HA2, HA3, HAS, HAS-1, HAS-2, HAS-3, HAS1, Has2, Has3, hasA, HASs, HsHAS1, HuHAS1, HyaD, hyaluronan synthase, hyaluronan synthase 1, hyaluronan synthase 2, hyaluronan synthase 3, hyaluronan synthase-1, hyaluronan synthase-2, hyaluronan synthases 2, hyaluronan synthethase, hyaluronate synthase, hyaluronate synthetase, hyaluronic acid synthase, hyaluronic acid synthetase, More, PmHAS, seHAS, XHAS1, XHAS2, XHAS3
ECTree
2.4.1.212 hyaluronan synthaseAdvanced search results
results (
results (KM Value
KM Value on EC 2.4.1.212 - hyaluronan synthase
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
top
KM VALUE [mM] 
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
IMAGE
0.7
hyaluronan oligomer HA4
with UDP-alpha-N-acetyl-D-glucosamine, pH 8.0, 35°C, recombinant enzyme
-
0.6
hyaluronan oligomer HA5
with UDP-alpha-D-glucuronate, pH 8.0, 35°C, recombinant enzyme
-
1
hyaluronan oligomer HA6
with UDP-alpha-N-acetyl-D-glucosamine, pH 8.0, 35°C, recombinant enzyme
-
0.7
hyaluronan oligomer HA8
with UDP-alpha-N-acetyl-D-glucosamine, pH 8.0, 35°C, recombinant enzyme
-
23.4
UDP-N-acetyl-alpha-D-glucosamine
pH 7.0, 25 °C, 10 mM MnCl2 and 5 mM UDP-GlcA (in absence of HA oligosaccharide as acceptor substrate)
0.8
UDP-alpha-D-glucuronate
pH 7.0, 25 °C, 10 mM MnCl2 and 15 mM UDP-GlcNAc (in absence of HA oligosaccharide as acceptor substrate)
0.04
UDP-D-glucuronate
-
pH 7.0, 30°C, recombinant mutant C281A
0.044
UDP-D-glucuronate
-
pH 7.0, 30°C, recombinant mutant C226S
0.056
UDP-D-glucuronate
-
pH 7.0, 30°C, recombinant mutant C281S
0.077
UDP-D-glucuronate
-
pH 7.0, 30°C, recombinant wild-type enzyme
0.079
UDP-D-glucuronate
-
pH 7.0, 30°C, recombinant mutant C367S
0.085
UDP-D-glucuronate
-
pH 7.0, 30°C, recombinant mutant C367A
0.088
UDP-D-glucuronate
-
pH 7.0, 30°C, recombinant mutant C226A
0.096
UDP-D-glucuronate
-
pH 7.0, 30°C, recombinant mutant C262S
0.11
UDP-D-glucuronate
-
pH 7.5, 30°C, recombinant wild-type enzyme at low UDP-N-acetyl-D-glucosamine concentration, and mutant C239S
0.146
UDP-D-glucuronate
-
pH 7.0, 30°C, recombinant mutant C262A
0.053
UDP-N-acetyl-D-glucosamine
-
pH 7.0, 30°C, recombinant mutant C226A/C281A
0.065
UDP-N-acetyl-D-glucosamine
-
pH 7.0, 30°C, recombinant mutant C281A/C367A
0.074
UDP-N-acetyl-D-glucosamine
-
pH 7.0, 30°C, recombinant wild-type enzyme
0.079
UDP-N-acetyl-D-glucosamine
-
pH 7.0, 30°C, recombinant mutant C226A/C367A
0.09
UDP-N-acetyl-D-glucosamine
-
pH 7.0, 30°C, recombinant mutant C367A
0.091
UDP-N-acetyl-D-glucosamine
-
pH 7.0, 30°C, recombinant mutant C367S
0.098
UDP-N-acetyl-D-glucosamine
-
pH 7.0, 30°C, recombinant mutant C281S
0.113
UDP-N-acetyl-D-glucosamine
-
pH 7.0, 30°C, recombinant mutant C262A/C281A
0.121
UDP-N-acetyl-D-glucosamine
-
pH 7.0, 30°C, recombinant mutant C262A/C367A
0.13
UDP-N-acetyl-D-glucosamine
-
pH 7.0, 30°C, recombinant mutant C281A
0.134
UDP-N-acetyl-D-glucosamine
-
pH 7.0, 30°C, recombinant mutant C226A/C262A
0.153
UDP-N-acetyl-D-glucosamine
-
pH 7.0, 30°C, recombinant mutant C262S
0.154
UDP-N-acetyl-D-glucosamine
-
pH 7.0, 30°C, recombinant mutant C226A
0.186
UDP-N-acetyl-D-glucosamine
-
pH 7.0, 30°C, recombinant mutant C262A
0.232
UDP-N-acetyl-D-glucosamine
-
pH 7.0, 30°C, recombinant mutant C226S
0.26
UDP-N-acetyl-D-glucosamine
-
pH 7.5, 30°C, recombinant wild-type enzyme at low UDP-glucuronate concentration
0.4
UDP-N-acetyl-D-glucosamine
-
pH 7.5, 30°C, recombinant wild-type enzyme and mutants C210S, C337S at low UDP-glucuronate concentration
0.88
UDP-N-acetyl-D-glucosamine
-
pH 7.5, 30°C, recombinant mutant C337S at higher UDP-glucuronate concentration
additional information
additional information
-
values for other substrate concentrations
-
additional information
additional information
-
kinetics, wild-type and mutant enzymes
-
additional information
additional information
-
kinetics, kinetic analysis of Cys-deletion mutants, overview
-
additional information
additional information
-
Km values of the enzyme in standard reaction in presence of diverse protecting nucleotide compounds, overview
-
additional information
additional information
-
recombinant enzyme, kinetics at different pH, thermodynamics
-
additional information
additional information
availability of substrate UDP-GlcNAc does not considerably influence the Km of Has1 toward UDP-GlcUA, whereas levels of UDP-GlcUA have a significant effect of the Km toward UDP-GlcNAc
-
additional information
additional information
structural and kinetic analysis and modeling, both NAc- and UA-transferase domains follow a sequential kinetic mechanism, most likely an ordered one in which the UDP-sugar donor binds first, followed by the HA oligosaccharide. After transfer of the sugar moiety, both products are released, first the elongated HA oligosaccharide and then the UDP sugar. A mechanistic shift from a steady-state ordered bi-bi to rapid equilibrium ordered bi-bi mechanism is observed at the NAc-site between the HA6 and HA8 elongation, detailed overview
-
