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2.4.1.214: glycoprotein 3-alpha-L-fucosyltransferase

This is an abbreviated version!
For detailed information about glycoprotein 3-alpha-L-fucosyltransferase, go to the full flat file.

Word Map on EC 2.4.1.214

Reaction

GDP-beta-L-fucose
+
N4-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc]-L-asparaginyl-[protein]
=
GDP
 +
N4-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-[alpha-L-Fuc-(1->3)]-beta-D-GlcNAc]-L-asparaginyl-[protein]

Synonyms

alpha 1,3-fucosyltransferase VI, alpha(1,3)-fucosyltransferase VII, alpha(1,3)-fucosyltransferase-IV, alpha(1,3)-fucosyltransferase-VII, alpha(1,3)fucosyltransferase, alpha-1,3-fucosyltransferase-VII, alpha1,3-fucosyltransferase, alpha1,3-fucosyltransferase 10, alpha1,3-fucosyltransferase 7, alpha1,3-fucosyltransferase 9, alpha1,3-fucosyltransferase IX, alpha1,3FTs, alpha1-3 fucosyltransferase-VII, alpha3-fucosyltransferase, alpha3-FucT VII, AtFUT11, CEFT-1, CEFT-2, CEFT-3, CEFT-4, CEFT-5, CEFT3, core alpha-(1,3)-fucosyltransferase, core alpha1,3-fucosyltransferase, Fuc-T C3, Fuc-TVII, fucosyltransferase A, fucosyltransferase VII, FucT, FucT 115, FucT 45, FucT VI, FucT VII, FucT-IV, FucT-VII, FucT1, FucTA, FucTB, FucTC, FUT-1, FUT-6, FUT10, FUT11, FUT7, Fut9, GDP-fucose:beta-N-acetylglucosamine (Fuc to (Fuca1-6GlcNAc)-Asn-peptide) alpha1-3-fucosyltransferase, GDP-L-fuc:Asn-linked GlcNAc alpha1,3-fucosyltransferase, GDP-L-fuc:N-acetyl-beta-D-glucosaminide alpha1,3-fucosyltransferase, LOC107797347, LOC107830046, NM_001324945, XM_016620229, XM_016657530

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.214 glycoprotein 3-alpha-L-fucosyltransferase

Cloned

Cloned on EC 2.4.1.214 - glycoprotein 3-alpha-L-fucosyltransferase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
1121-bp PCR product of cDNA containing the full length of the FUT7 gene ligated into the pM.D.18-T cloning vector, propagation in Escherichia coli DH5alpha. Full coding sequence of FUT7 subcloned into pIRES2-EGFP expression vector and transiently transfected into RL95-2 cells or injected into the uteri of early pregnant mice
cDNA cloning of Fuc-T C3 from mRNA, recombinant enzyme expressed in Sf21 insect cells using baculovirus vector
-
expressed in Pichia pastoris
-
expressed in Pichia pastoris GS115 cells
expressed in Pichia pastoris strain GS115
expressed in Spodoptera frugiperda Sf9 cells
-
expression in CHO cells
-
expression in COS7 cells, fusion of CFET-1 to green fluorescent protein
-
expression in Escherichia coli DH5alpha or BL21
expression in Pichia pastoris GS115 cells
expression in sf21 cell
expression in Sf9 cell
expression in Sf9 cells
expression of FucT-VII in H7721 hepatocarcinoma cells
-
expression of the soluble enzyme, by constructing a fusion enzyme amino acid residues 1-63 of a mammalian enzyme fused to the stem and the catalytic region of residues 39-342 of the enzyme, in CHO cells
-
expression of wild-type and mutant FucT VIs in COS-7 cells as soluble N-terminal protein A-fusion proteins, mutant K241A shows a low expression level
-
expression of wild-type enzyme, in Pichia pastoris strain GS115
-
FucT cDNA (414 bp) amplified and cloned into the BamHI site of puc18XTI2 to create puc18Fsi. The antisense fragment amplified and cloned into the KpnI digested vector puc18Fsi to generate puc18Fsias. Subsequently, the 'sense-intron-antisense' cassette excised by XbaI digestion and cloned into XbaI linearized plant expression vector pGA643
-
gene FUT7, stable expression in MOLT-4 8H cells being induced by the recombinant enzyme in presence of mifepristone to produce sL2x epitopes, expression of Staphylococcus aureus protein A-human FucT-VII fusion protein in Spodoptera frugiperda Sf21 insect cells via the baculovirus infection system
-
overexpression Escherichia coli to obtain a selenomethionine-labeled L202M FucT for crystallization
-
the Apis mellifera genome encodes three alpha1,3-FucT homologues FucTA, FucTB and FucTC, expressed in yeast and insect cells only FucTA is found to be a core alpha1,3-FucT (EC 2.1.214), FucTC discloses to be the first Lewis-type alpha1,3-FucT (EC 2.4.1.152) to be described in insects, FucTB does not show any activity
the complex of the FUT7-cytoplasmic tail and bovine UDP-galactose:beta-N-acetylglucosaminide: beta1,4-galactosyltransferase 1 is expressed in Sf9 insect cells
three genes FucTA, FucTB and FucTC encode proteins similar to alpha1-3-fucosyltransferases, expression in Pichia pastoris