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2.4.1.214: glycoprotein 3-alpha-L-fucosyltransferase

This is an abbreviated version!
For detailed information about glycoprotein 3-alpha-L-fucosyltransferase, go to the full flat file.

Word Map on EC 2.4.1.214

Reaction

GDP-beta-L-fucose
+
N4-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc]-L-asparaginyl-[protein]
=
GDP
+
N4-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-[alpha-L-Fuc-(1->3)]-beta-D-GlcNAc]-L-asparaginyl-[protein]

Synonyms

alpha 1,3-fucosyltransferase VI, alpha(1,3)-fucosyltransferase VII, alpha(1,3)-fucosyltransferase-IV, alpha(1,3)-fucosyltransferase-VII, alpha(1,3)fucosyltransferase, alpha-1,3-fucosyltransferase-VII, alpha1,3-fucosyltransferase, alpha1,3-fucosyltransferase 10, alpha1,3-fucosyltransferase 7, alpha1,3-fucosyltransferase 9, alpha1,3-fucosyltransferase IX, alpha1,3FTs, alpha1-3 fucosyltransferase-VII, alpha3-fucosyltransferase, alpha3-FucT VII, AtFUT11, CEFT-1, CEFT-2, CEFT-3, CEFT-4, CEFT-5, CEFT3, core alpha-(1,3)-fucosyltransferase, core alpha1,3-fucosyltransferase, Fuc-T C3, Fuc-TVII, fucosyltransferase A, fucosyltransferase VII, FucT, FucT 115, FucT 45, FucT VI, FucT VII, FucT-IV, FucT-VII, FucT1, FucTA, FucTB, FucTC, FUT-1, FUT-6, FUT10, FUT11, FUT7, Fut9, GDP-fucose:beta-N-acetylglucosamine (Fuc to (Fuca1-6GlcNAc)-Asn-peptide) alpha1-3-fucosyltransferase, GDP-L-fuc:Asn-linked GlcNAc alpha1,3-fucosyltransferase, GDP-L-fuc:N-acetyl-beta-D-glucosaminide alpha1,3-fucosyltransferase, LOC107797347, LOC107830046, NM_001324945, XM_016620229, XM_016657530

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.214 glycoprotein 3-alpha-L-fucosyltransferase

Purification

Purification on EC 2.4.1.214 - glycoprotein 3-alpha-L-fucosyltransferase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
by chromatography on DE52 cellulose and Affigel Blue, chromatofocusing using PBE 94 and polybuffer 96, gelfiltration on a Sephacryl S200 column and affinity chromatography on a GnGn-column
-
Ni-NTA column chromatography
-
Ni-NTA column chromatography and Affi-Gel Blue Sepharose column chromatography
Ni-NTA or HisTrap column chromatography
of Fuc-T C3, using Triton X-100 extraction and column chromatography on DE52 cellulose, Affi-Gel blue, S-Sepharose, GnGn-Sepharose and GDP-hexanolamine-Sepharose
-
recombinant soluble enzyme from CHO cells by GDP-hexanolamine affinity chromatography to homogeneity
-
recombinant soluble protein A-fusion wild-type and mutant FucT VIs from COS-7 cells by IgG affinity chromatography
-