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2.4.1.217: mannosyl-3-phosphoglycerate synthase

This is an abbreviated version!
For detailed information about mannosyl-3-phosphoglycerate synthase, go to the full flat file.

Word Map on EC 2.4.1.217

Reaction

GDP-mannose
+
3-phospho-D-glycerate
=
GDP
 +
2-(alpha-D-mannosyl)-3-phosphoglycerate

Synonyms

mannosyl-3-P-glycerate synthase, mannosyl-3-phosphoglycerate phosphatase, mannosyl-3-phosphoglycerate synthase, mannosyl-3-phosphoglycerate synthase/phosphatase, mannosylglycerate synthase, MGSD, MPG synthase, MPGP, MPGS, MPGS/GPGS, MpgS/P, synthase, mannosyl-3-phosphoglycerate

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.217 mannosyl-3-phosphoglycerate synthase

Cloned

Cloned on EC 2.4.1.217 - mannosyl-3-phosphoglycerate synthase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
corresponding gene is expressed in Escherichia coli
DNA and amino acid sequence determination and analysis, genetic organization of genes involved in mannosylglycerate biosynthesis, functional overexpression in Escherichia coli strain BL21
expressed in Escherichia coli
expressed in Escherichia coli BL21 Rosetta cells
-
gene mgsD, DNA and amino acid sequence determination and analysis, genetic organization, overexpression of the holoenzyme and of the isolated mannosyl-3-phosphoglycerate synthase and mannosyl-3-phosphoglycerate phosphatase domains of the bifunctional enzyme in Escherichia coli, the recombinant Escherichia coli strain does not accumulate mannosylglycerate, expression of active bifunctional enzyme in Saccharomyces cerevisiae
-
gene mpgs, DNA and amino acid sequence determination and analysis of the 2 isozymes, expression in Escherichia coli strain BL21(DE)
into the pGEMT-Easy vector for sequencing and subcloned into pET30a for expression in Escherichia coli BL21DE3 cells
overexpressed in Escherichia coli
overexpression in Escherichia coli
-
phylogenetic analysis
the mpgS gene from strain HB27 is cloned into the pGEM-T Easy vector