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2.4.1.225: N-acetylglucosaminyl-proteoglycan 4-beta-glucuronosyltransferase

This is an abbreviated version!
For detailed information about N-acetylglucosaminyl-proteoglycan 4-beta-glucuronosyltransferase, go to the full flat file.

Word Map on EC 2.4.1.225

Reaction

UDP-alpha-D-glucuronate
+
N-acetyl-alpha-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-proteoglycan
=
UDP
 +
beta-D-glucuronosyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-proteoglycan

Synonyms

diphosphoglucuronate:oligosaccharide uridine glucuronosyltransferase, exostosin, exostosin-1, exostosin-2, Exostosin1, Exostosin2, exotose-2, EXT-1, EXT-2, EXT-3, EXT1, EXT2, EXTL3, extosin, gene EXTL1 glycosyltransferase, gene EXTL2 glycosyltransferase, glucuronic acid/N-acetylglucosamine co-polymerase-1, glucuronosyltransferase, heparan glucuronosyltransferase II, heparan sulfate co-polymerase, heparan sulfate glucuronosyltransferase, heparan sulfate synthesis enzyme, N-acetylglucosaminylproteoglycan beta-1,4-glucuronosyltransferase, RIB-1, RIB-2, sister of tout velu, sister of tout-velu, sotv, tout velu, tout-velu, ttv, UDP-glucuronate:oligosaccharide

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.225 N-acetylglucosaminyl-proteoglycan 4-beta-glucuronosyltransferase

Cloned

Cloned on EC 2.4.1.225 - N-acetylglucosaminyl-proteoglycan 4-beta-glucuronosyltransferase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Drosophila melanogaster
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expressed in HEK-293 cells
expression in CHO cells
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expression in COS-7 cells as His/FLAG fusion protein
EXT1 and EXT2 cDNA, alone or in combination, cloned into the pBudCE4.1 vector, which contains two multiple cloning sites for simultaneous expression of two proteins. EXT1 cloned into the multiple cloning sites with the cytomegalovirus (CMV) immediate-early promotor and EXT2 into the multiple cloning sites with the human elongation factor 1alpha promotor. Constructs transfected into HEK-293 cells stably expressing NDST1 from the pCDNA3 vector. Mutated EXT2 cDNA cloned into the pBudCE4.1 vector and transfected into HEK-93 cells overexpressing NDST1. EXT2 cDNA inserted into the pCAGGS expression vector under the control of the CMV immediate-early enhancer and the chicken beta-actin promoter (CAG) for constitutively high tissue expression from fertilized eggs and early embryonic stage through adulthood. The cDNA construct cloned into the unique EcoR1 site between the CAG promoter and the rabbit beta-globin sequence. Vector linearized with BamH1 and SalI and injected into mice B6CBAF1 oocytes
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full-length Ext1 cDNA cloned into the pBudCE4.1 vector and transfected into Ext1Gt/Gt fibroblasts
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gene EXT1, creation of loxP-modified Ext1 allele and establishment of the Ext1 floxed mouse line (Ext1flox/flox), expression of the enzyme in Gdf-5-Cre transgenic mice, semiquantitative real-time PCR enzyme expression analysis
gene EXT1, real-time quantitative PCR enzyme expression analysis
gene EXT1, recombinant expression of Myc-DKK-tagged enzyme in S2 cells, interaction analysis with GOLPH3 via yeast two-hybrid assay
gene EXT2, genotyping, autozygosity mapping
gene EXT2, recombinant expression of Myc-DKK-tagged enzyme in S2 cells, interaction analysis with GOLPH3 via yeast two-hybrid assay