2.4.1.231: alpha,alpha-trehalose phosphorylase (configuration-retaining)
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For detailed information about alpha,alpha-trehalose phosphorylase (configuration-retaining), go to the full flat file.
Reaction
Synonyms
PoTPase, ScTPase, TP2, TP3, trehalose phosphorylase
ECTree
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General Stability
General Stability on EC 2.4.1.231 - alpha,alpha-trehalose phosphorylase (configuration-retaining)
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highly unstable during purification, glycerol, phosphate, dithiothreitol and PMSF stabilize
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unstable protein, half-life of no more than approximately 1.3 h in 20mM MES buffer, 25°C, pH 7.0. The enzyme can be stabilized on addition of alpha,alpha-trehalose (300 mM) which yields a half-life of 11.5 h. In the presence of 20% (w/v) glycerol, and 9 and 26% (w/v) PEG-4000, half-life is 6.5 h, 16 h and 70 h, respectively. Large improvements in stability by covalent protein modification with activated derivatives of PEG-5000
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