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2.4.1.232: initiation-specific alpha-1,6-mannosyltransferase

This is an abbreviated version!
For detailed information about initiation-specific alpha-1,6-mannosyltransferase, go to the full flat file.

Word Map on EC 2.4.1.232

Reaction

GDP-mannose
+
Man8GlcNAc
=
GDP
+
Man9GlcNAc

Synonyms

AFUA_2G01450, alpha-1,6-mannosyltransferase, alpha-1,6-MT, alpha-mannosyltransferase, alpha1,6-mannosyltransferase KlOch1p, alpha1-6-mannosyltransferase, Anl1p, Anp1p, Dol-P-Man:Man7GlcNAc2-PP-Dol-alpha1-6-mannosyltransferase, GDP-mannose:glycolipid 1,6-alpha-D-mannosyltransferase, GDP-mannose:oligosaccharide 1,6-alpha-D-mannosyltransferase, glycolipid 6-alpha-mannosyltransferase, glycoprotein alpha1-6-mannosyltransferase, HpOCH1, initiation-specific alpha-1,6-mannosyltransferase, mannosyltransferase, guanosine diphosphomannose-glycoprotein alpha1-6-, Mnn9, Mnn9p, och1+, Och1p, ScMnn9p, SpOch1p, TbALG12, VdOCH1, YlOch1p

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.232 initiation-specific alpha-1,6-mannosyltransferase

Cloned

Cloned on EC 2.4.1.232 - initiation-specific alpha-1,6-mannosyltransferase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
ANL1 and OCH1 gene
-
as a fusion with the with green fluorescent protein, MNN9-OCH1 chimeric genes are constructed and cloned
-
as a fusion with the with red fluorescent protein, MNN9-OCH1 chimeric genes are constructed and cloned
-
into the pGEMT-Easy vector
-
MNN9 gene encoding Mnn9p is cloned and sequenced
-
OCH1 gene encodes Och1p, an alpha-1,6-mannosyltransferase
-
och1+ gene encodes alpha-1,6-mannosyltransferase, recombinant expression and cloning, och1+ gene knockout fission yeast
-
och1+ gene, sequencing
recombinant expression of His6-tagged wild-type and mutant enzymes in Escherichia coli BL21(DE3) pLysS, expression of mutant enzymes in Saccharomyces cerevisiae strain BY4741 DELTAMNN9
recombinant Och1p is expressed in Pichia pastoris as a secreted protein, after truncation of its transmembrane region and fusion with myc and histidine tags at the C-terminus
-
transcription of the OCH1 gene encoding alpha-1,6-mannosyltransferase is regulated by 2 transcription factors, Swi4 and Skn7, it is controlled by the ubiquitin-dependent degradation pathway
-
transcriptional control of the och1+ gene, gene expression is not regulated during the cell cycle, but is induced by NaCl and KCl through the transcription factor Atf1p
-