2.4.1.238: delphinidin 3,5-di-O-glucoside 3'-O-glucosyltransferase
This is an abbreviated version!
For detailed information about delphinidin 3,5-di-O-glucoside 3'-O-glucosyltransferase, go to the full flat file.
Reaction
Synonyms
3'GT, anthocyanin 3'-O-beta-D-glucosyltransferase, UDP-glucose:anthocyanin 3'-O-glucosyltransferase
ECTree
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Substrates Products
Substrates Products on EC 2.4.1.238 - delphinidin 3,5-di-O-glucoside 3'-O-glucosyltransferase
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REACTION DIAGRAM
UDP-alpha-D-glucose + delphinidin 3-O-glucosyl-5-O-(6-O-caffeoylglucoside)
UDP + delphinidin 3-O,3'-O-di-beta-D-glucosyl-5-O-(6-O-caffeoyl-beta-D-glucoside)
in vitro, 50.9% of the activity with delphinidin 3,5-diglucoside
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UDP-glucose + an anthocyanidin
UDP + an anthocyanidin 3'-O-beta-D-glucoside
strict substrate specificity, 3'GT specifically glucosylates the 3'-hydroxy group of delphinidin-type anthocyanins containing glucose groups at the 3 and 5 positions and specifically uses UDP-glucose as the sugar donor, 3GT strictly requires three hydroxy groups on the B-ring for its substrates
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UDP-glucose + delphinidin
UDP + delphinidin 3'-glucoside
poor substrate, 3.3% of the activity with delphinidin 3,5-diglucoside
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UDP-glucose + delphinidin 3,5-di-O-beta-D-glucoside
UDP + delphinidin 3,5,7-tri-O-beta-D-glucoside
3'GT glucosylates the 3'-hydroxy group of delphinidin 3,5-diglucoside, 2fold higher activity than with delphinidin 3-O-glucosyl-5-O-(6-O-caffeoylglucoside) as substrate
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UDP-glucose + delphinidin 3-glucoside
UDP + delphinidin 3,3'-diglucoside
poor substrate, 2.9% of the activity with delphinidin 3,5-diglucoside
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key enzyme for blue anthocyanin biosynthesis, biosynthesis of gentiodelphin
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additional information
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no sugar donors: UDP-galactose, UDP-GlcUA, UDP-N-acetylgalactosamine, not: pelargonidin 3,5-diglucoside, cyanidin 3,5-diglucoside, dihydroflavonols, dihydrokaempferol, dihydroquercetin, dihydromyricetin, flavone, tricetin
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