2.4.1.240: flavonol-3-O-glycoside glucosyltransferase
This is an abbreviated version!
For detailed information about flavonol-3-O-glycoside glucosyltransferase, go to the full flat file.
Reaction
Synonyms
UDP-glucose:glucosyltransferase
ECTree
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Substrates Products
Substrates Products on EC 2.4.1.240 - flavonol-3-O-glycoside glucosyltransferase
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REACTION DIAGRAM
TDP-glucose + a flavonol 3-O-beta-D-glucosyl-(1-2)-beta-D-glucoside
TDP + a flavonol 3-O-beta-D-glucosyl-(1-2)-beta-D-glucosyl-(1-2)-beta-D-glucoside
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3fold lower rate than with UDP-glucose as glucosyl donor
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UDP-glucose + a flavonol 3-O-beta-D-glucosyl-(1-2)-beta-D-glucoside
UDP + a flavonol 3-O-beta-D-glucosyl-(1-2)-beta-D-glucosyl-(1-2)-beta-D-glucoside
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highly specific enzyme, specific for flavonol-3-O-diglucosides, 3fold higher rate than with TDP-glucose as glucosyl donor
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UDP-glucose + kaempferol 3-O-beta-D-glucosyl-(1-2)-beta-D-glucoside
UDP + kaempferol 3-O-beta-D-glucosyl-(1-2)-beta-D-glucosyl-(1-2)-beta-D-glucoside
UDP-glucose + myricetin 3-O-beta-D-glucosyl-(1-2)-beta-D-glucoside
UDP + myricetin 3-O-beta-D-glucosyl-(1-2)-beta-D-glucosyl-(1-2)-beta-D-glucoside
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32% of the activity with kaempferol 3-O-beta-D-glucosyl-(1-2)-beta-D-glucoside
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UDP-glucose + quercetin 3-O-beta-D-glucosyl-(1-2)-beta-D-glucoside
UDP + quercetin 3-O-beta-D-glucosyl-(1-2)-beta-D-glucosyl-(1-2)-beta-D-glucoside
UDP + kaempferol 3-O-beta-D-glucosyl-(1-2)-beta-D-glucosyl-(1-2)-beta-D-glucoside
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UDP-glucose + kaempferol 3-O-beta-D-glucosyl-(1-2)-beta-D-glucoside
UDP + kaempferol 3-O-beta-D-glucosyl-(1-2)-beta-D-glucosyl-(1-2)-beta-D-glucoside
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best substrate
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UDP + quercetin 3-O-beta-D-glucosyl-(1-2)-beta-D-glucosyl-(1-2)-beta-D-glucoside
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UDP-glucose + quercetin 3-O-beta-D-glucosyl-(1-2)-beta-D-glucoside
UDP + quercetin 3-O-beta-D-glucosyl-(1-2)-beta-D-glucosyl-(1-2)-beta-D-glucoside
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14% of the activity with kaempferol 3-O-beta-D-glucosyl-(1-2)-beta-D-glucoside
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involved in the biosynthesis of flavonol triglucosides
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additional information
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one of three glucosyl transferases involved in the biosynthesis of flavonol-3-triglucosides
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additional information
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glucosylation of kaempferol and quercetin in the 3-position to form the 3-O-triglucoside, reaction proceeds from the aglycone via the mono- and diglucoside intermediates, this reaction sequence is probably associated with three distinct enzyme activities
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additional information
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no glucosylation of pelargonidin, naringenin, naringin and p-coumaric acid by the three flavonol:glucosyltransferases, not: ADP-glucose, GDP-glucose, kaempferol, kaempferol monoglucoside, quercetin-3-rutinoside
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