2.4.1.245: alpha,alpha-trehalose synthase

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For detailed information about alpha,alpha-trehalose synthase, go to the full flat file.

Reaction

Synonyms

PH1035, TaTS, TmTreT, trehalose glycosyltransferring synthase, Trehalose synthase, TreT

ECTree

2 Transferases

2.4 Glycosyltransferases

2.4.1 Hexosyltransferases

2.4.1.245 alpha,alpha-trehalose synthase

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Results	in table
320	AA Sequence
7	Application
1	CAS Registry Number
7	Cloned(Commentary)
1	Crystallization (Commentary)
1	General Information
9	Inhibitors
13	kcat/KM [mM/s]
24	KM Value [mM]
13	Metals/Ions
10	Molecular Weight [Da]
1	Natural Substrates/ Products (Substrates)
23	Organism
5	Pathway
7	pH Optimum
2	pH Range
2	pH Stability
14	Protein Variants
3	Purification (Commentary)
2	Reaction
1	Reaction Type
14	Reference
48	Substrates and Products (Substrate)
4	Subunits
16	Synonyms
1	Systematic Name
7	Temperature Optimum [°C]
3	Temperature Range [°C]
4	Temperature Stability [°C]
13	Turnover Number [1/s]

Crystallization

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Crystallization on EC 2.4.1.245 - alpha,alpha-trehalose synthase

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purified enzyme free or as TreT-UDP binary complex, 10 mg/ml native enzyme from PEG 3350 25%, 0.2 M MgCl2, and 0.1 M sodium HEPES, 18°C, the selenomethionine-substituted protein crystal grow from 21% methoxy PEG 2000, 0.18 M ammonium sulfate, and 0.1 M sodium acetate, pH 4.6, for the UDP-glucose complex crystal, 5 mM UDPG is added to 10 mg/ml E326A protein for 1 h prior to setup of the crystallization using the same conditions as for the native crystal, X-ray diffraction structure determination and analysis at 2.3-3.0 A resolution, single-wavelength anomalous dispersion phasing

Pyrococcus horikoshii

O58762

722972