2.4.1.247: beta-D-galactosyl-(1->4)-L-rhamnose phosphorylase

This is an abbreviated version!
For detailed information about beta-D-galactosyl-(1->4)-L-rhamnose phosphorylase, go to the full flat file.

Reaction

Synonyms

cphy1920 protein, D-galactosyl-beta-1,4-L-rhamnose phosphorylase, D-galactosyl-beta1->4-L-rhamnose phosphorylase, GalRhaP, Oter 1377 protein

ECTree

     2 Transferases

         2.4 Glycosyltransferases

             2.4.1 Hexosyltransferases

                2.4.1.247 beta-D-galactosyl-(1->4)-L-rhamnose phosphorylase

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Results	in table
44	AA Sequence
1	Application
2	Cloned(Commentary)
2	General Information
7	kcat/KM [mM/s]
15	KM Value [mM]
1	Localization
4	Molecular Weight [Da]
1	Natural Substrates/ Products (Substrates)
2	Organism
2	pH Optimum
1	pH Range
1	pH Stability
2	Purification (Commentary)
1	Reaction
2	Reference
8	Specific Activity [micromol/min/mg]
18	Substrates and Products (Substrate)
1	Subunits
6	Synonyms
1	Systematic Name
1	Temperature Optimum [°C]
1	Temperature Range [°C]
2	Temperature Stability [°C]
15	Turnover Number [1/s]

Substrates Products

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of the Enzyme Summary Page.

Substrates Products on EC 2.4.1.247 - beta-D-galactosyl-(1->4)-L-rhamnose phosphorylase

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SUBSTRATE

PRODUCT                       

REACTION DIAGRAM

ORGANISM

UNIPROT

COMMENTARY
(Substrate)

LITERATURE
(Substrate)

COMMENTARY
(Product)

LITERATURE
(Product)

Reversibility
r=reversible
ir=irreversible
?=not specified

2-deoxy-D-glucose + alpha-D-galactose 1-phosphate

beta-D-galactosyl-(1->4)-2-deoxy-D-glucose + phosphate

Opitutus terrae

-

reverse (synthetic) reaction

722132

-

-

r

2-deoxy-D-glucose + alpha-D-galactose 1-phosphate

D-galactosyl-beta-1,3-2-deoxy-D-glucose + phosphate

Lachnoclostridium phytofermentans

A9KHK4

when D-glucose derivatives are used as acceptors, reaction products are beta-1,3-galactosides

693235

-

-

r

alpha-D-galactose 1-phosphate + L-rhamnose

D-galactosyl-beta-1,4-L-rhamnose + phosphate

Lachnoclostridium phytofermentans

A9KHK4

highest activity

693235

-

-

r

beta-D-galactosyl-(1->3)-D-glucose + phosphate

D-glucose + alpha-D-galactose 1-phosphate

Opitutus terrae

-

lower activity

722132

-

-

r

beta-D-galactosyl-(1->4)-L-rhamnose + phosphate

L-rhamnose + alpha-D-galactose 1-phosphate

2 entries

D-galactose + alpha-D-galactose 1-phosphate

beta-D-galactosyl-(1->4)-D-galactose + phosphate

Opitutus terrae

-

reverse (synthetic) reaction

722132

-

-

r

D-galactose + alpha-D-galactose 1-phosphate

D-galactosyl-beta-1,3-D-galactose + phosphate

Lachnoclostridium phytofermentans

A9KHK4

-

693235

-

-

r

D-glucose + alpha-D-galactose 1-phosphate

beta-D-galactosyl-(1->4)-D-glucose + phosphate

Opitutus terrae

-

reverse (synthetic) reaction

722132

-

-

r

D-glucose + alpha-D-galactose 1-phosphate

D-galactosyl-beta-1,3-D-glucose + phosphate

Lachnoclostridium phytofermentans

A9KHK4

when D-glucose derivatives are used as acceptors, reaction products are beta-1,3-galactosides

693235

-

-

r

D-xylose + alpha-D-galactose 1-phosphate

beta-D-galactosyl-(1->4)-D-xylose + phosphate

Opitutus terrae

-

reverse (synthetic) reaction

722132

-

-

r

L-lyxose + alpha-D-galactose 1-phosphate

beta-D-galactosyl-(1->4)-L-lyxose + phosphate

2 entries

L-mannose + alpha-D-galactose 1-phosphate

beta-D-galactosyl-(1->4)-L-mannose + phosphate

Opitutus terrae

-

reverse (synthetic) reaction

722132

-

-

r

L-mannose + alpha-D-galactose 1-phosphate

D-galactosyl-beta-1,4-L-mannose + phosphate

Lachnoclostridium phytofermentans

A9KHK4

-

693235

-

-

r

L-rhamnose + alpha-D-galactose 1-phosphate

beta-D-galactosyl-(1->4)-L-rhamnose + phosphate

Opitutus terrae

-

reverse (synthetic) reaction

722132

-

-

r

additional information

?

-

3 entries

beta-D-galactosyl-(1->4)-L-rhamnose + phosphate

L-rhamnose + alpha-D-galactose 1-phosphate

Opitutus terrae

-

-

722132

-

-

?

beta-D-galactosyl-(1->4)-L-rhamnose + phosphate

L-rhamnose + alpha-D-galactose 1-phosphate

Opitutus terrae

-

preferred substrate

722132

-

-

r

L-lyxose + alpha-D-galactose 1-phosphate

beta-D-galactosyl-(1->4)-L-lyxose + phosphate

Lachnoclostridium phytofermentans

A9KHK4

-

693235

-

-

r

L-lyxose + alpha-D-galactose 1-phosphate

beta-D-galactosyl-(1->4)-L-lyxose + phosphate

Opitutus terrae

-

reverse (synthetic) reaction

722132

-

-

r

additional information

?

-

Lachnoclostridium phytofermentans

A9KHK4

phosphorylyzes neither galacto-N-biose (D-Gal-beta1,3-D-GalNAc) nor lacto-N-biose I(D-Gal-beta1,3-D-GlcNAc), thus indicating that Cphy1920 protein is not GalHexNAcP

693235

-

-

?

additional information

?

-

Lachnoclostridium phytofermentans

-

phosphorylyzes neither galacto-N-biose (D-Gal-beta1,3-D-GalNAc) nor lacto-N-biose I(D-Gal-beta1,3-D-GlcNAc), thus indicating that Cphy1920 protein is not GalHexNAcP

693235

-

-

?

additional information

?

-

Opitutus terrae

-

in the reverse reaction, the enzyme shows higher activity on L-rhamnose derivatives than on D-glucose derivatives, no activity with lactose. The enzyme only exhibits activity on Gal 1-phosphate as a donor and not on alpha-D-glucose 1-phosphate and alpha-N-acetyl-D-glucosamine 1-phosphate. Various acceptors in the presence of Gal 1-P act as a donor using a reverse (synthetic) reaction with highest activity on L-Rha.The enzyme also exhibits activity on derivatives of L-Rha, but Oter 1377 protein shows no or very poor activity on D-GlcNAc, D-GalNAc, D-Man, D-glucosamine, L-Glc, D-Fru, D-Rib, L-Fuc, L-Ara, D-Lyx, D-glucal, sucrose, or maltose, substrate specificity, overview

722132

-

-

?