Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

2.4.1.248: cycloisomaltooligosaccharide glucanotransferase

This is an abbreviated version!
For detailed information about cycloisomaltooligosaccharide glucanotransferase, go to the full flat file.

Word Map on EC 2.4.1.248

Reaction

cyclizes part of a (1->6)-alpha-D-glucan chain by formation of a (1->6)-alpha-D-glucosidic bond =

Synonyms

cit, CITase, CITase-598K, CITase-T3040, isocyclomaltooligosaccharide glucanotransferase

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.248 cycloisomaltooligosaccharide glucanotransferase

Crystallization

Crystallization on EC 2.4.1.248 - cycloisomaltooligosaccharide glucanotransferase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
crystal structure of the core fragment from Ser39 to Met738, devoid of its N-terminal signal peptide and C-terminal nonconserved regions. The structural model contains one catalytic (beta/alpha)8-barrel domain and three beta-domains. Domain N with an immunoglobulin-like beta-sandwich fold is attached to the N-terminus. Domain C with a Greek key beta-sandwich fold is located at the C-terminus, and a carbohydrate-binding module family 35 beta-jellyroll domain B is inserted between the 7thbeta-strand and the 7th alpha-helix of the catalytic domain A. The structures of the inactive catalytic nucleophile mutant enzyme complexed with isomaltohexaose, isomaltoheptaose, isomaltooctaose, and cycloisomaltooctaose reveal that the ligands bind in the catalytic cleft and the sugar-binding site of CBM35
crystals of CITase bearing an N-terminal His6 tag, resolution of 2.3 A, belong to space group P3121, containing a single molecule in the asymmetric unit.. Crystals of CITase bearing a C-terminal His6 tag, resolution of 1.9 A, belong to space group P212121, containing two molecules in the asymmetric unit
sitting-drop vapour diffusion method at 20°C, crystal structure of the enzyme is determined in its apo- and cycloisomaltooligosaccharides (CI-7) complex forms