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2.4.1.248: cycloisomaltooligosaccharide glucanotransferase

This is an abbreviated version!
For detailed information about cycloisomaltooligosaccharide glucanotransferase, go to the full flat file.

Word Map on EC 2.4.1.248

Reaction

cyclizes part of a (1->6)-alpha-D-glucan chain by formation of a (1->6)-alpha-D-glucosidic bond =

Synonyms

cit, CITase, CITase-598K, CITase-T3040, isocyclomaltooligosaccharide glucanotransferase

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.248 cycloisomaltooligosaccharide glucanotransferase

Purification

Purification on EC 2.4.1.248 - cycloisomaltooligosaccharide glucanotransferase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant extracellular CITase from protease-deficient Bacillus subtilis strain 168 by ammonium sulfate fractionation, anion exchange chromatography, and gel filtration and another step of anion exchange chromatography, recombinant extracellular CITase from Bacillus circulans G22-10 by ammonium sulfate fractionation, anion exchange chromatography, and gel filtration
the C-terminal region of TtCITase (TtCITase-C, Met740-Phe1559), including a CBM35-like region and the GH family 15 domain. The domain is successfully cloned, expressed, and purified
WP_028992696