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2.4.1.25: 4-alpha-glucanotransferase

This is an abbreviated version!
For detailed information about 4-alpha-glucanotransferase, go to the full flat file.

Word Map on EC 2.4.1.25

Reaction

maltononaose
+
maltotriose
=
maltoundecaose
 +
D-glucose

Synonyms

(1->4)-alpha-D-glucan:(1->4)-alpha-D-glucan 4-alpha-D-glycosyltransferase, 4-alpha-D-alpha-glucanotransferase, 4-alpha-glucanotransferase, 4-alpha-GTase, 4alphaGT, 4alphaGTase, AgtA, AgtB, alpha-1,4-glucanotransferase, alpha-1,4-transferase, alpha-amylase-like transglycosylase, alpha-glucanotransferase, alphaGT, alphaGTase, AMase, amylmaltase, amylomaltase, At2g40840, CGTase, Cqm1, D-enzyme, debranching enzyme, debranching enzyme maltodextrin glycosyltransferase, dextrin glycosyltransferase, dextrin glycosyltransferase,, dextrin transglycosylase, disproportionating enzyme, DPE1, DPE2, EC 2.4.1.3, exo-MGTase, GDE, GH77, GH77 amylomaltase, glycogen debranching enzyme, GTase, MalQ, maltodextrin glycosyltransferase, maltosyltransferase, MgtA, MmtA, More, MQ-01, MTase, oligo-1,4-1,4-glucantransferase, PyAMase, TAalphaGT, TAalphaGTase, TLGT, TmalphaGT, TreX, TS alpha GTase, TSalphaGT

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.25 4-alpha-glucanotransferase

Purification

Purification on EC 2.4.1.25 - 4-alpha-glucanotransferase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
both amylomaltase and D-enzyme
-
DEAE column chromatography and phenyl column chromatography
E. coli ML 30, partially
-
HisTrap affinity column chromatography
HisTrap affinity column chromatography, and gel filtration
-
HisTrap column chromatography
HisTrap column chromatography, and gel filtration
-
iminodiacetic acid-Sepharose column chromatography and Superdex 200 gel filtration
native and recombinant enzyme
Ni HiTrap column chromatography and Superdex 75 gel filtration
Ni-IDA matrix chromatography
Ni-NTA agarose column chromatography and Superdex S200 gel filtration
Ni-NTA column chromatography
-
Ni-NTA column chromatography, and gel filtration
partial
recombinant enzyme
recombinant His-tagged alpaGTase 2.9fold from Escherichia coli strain BL21 (DE3) by nickel affinity chromatography
-
recombinant His-tagged enzyme by nickel affinity chromatography
-
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by heat treatment and nickel affinity chromatography, removal of the His-tag through cleavage with bovine thrombin
recombinant His-tagged enzyme from Escherichia coli strain MC1061 by nickel affinity chromatography
-
recombinant His-tagged TsalphaGT from Escherichia coli strain MC1061 by nickel affinity chromatography to homogeneity
-
recombinant His6-tagged MalQ from Escherichia coli strain BL21 (DE3) by nickel affinity chromatography
recombinant wild-type and mutant enzymes from Escherichia coli by nickel affinity chromatography