2.4.1.25: 4-alpha-glucanotransferase

This is an abbreviated version!
For detailed information about 4-alpha-glucanotransferase, go to the full flat file.

Reaction

Synonyms

(1->4)-alpha-D-glucan:(1->4)-alpha-D-glucan 4-alpha-D-glycosyltransferase, 4-alpha-D-alpha-glucanotransferase, 4-alpha-glucanotransferase, 4-alpha-GTase, 4alphaGT, 4alphaGTase, AgtA, AgtB, alpha-1,4-glucanotransferase, alpha-1,4-transferase, alpha-amylase-like transglycosylase, alpha-glucanotransferase, alphaGT, alphaGTase, AMase, amylmaltase, amylomaltase, At2g40840, CGTase, Cqm1, D-enzyme, debranching enzyme, debranching enzyme maltodextrin glycosyltransferase, dextrin glycosyltransferase, dextrin glycosyltransferase,, dextrin transglycosylase, disproportionating enzyme, DPE1, DPE2, EC 2.4.1.3, exo-MGTase, GDE, GH77, GH77 amylomaltase, glycogen debranching enzyme, GTase, MalQ, maltodextrin glycosyltransferase, maltosyltransferase, MgtA, MmtA, More, MQ-01, MTase, oligo-1,4-1,4-glucantransferase, PyAMase, TAalphaGT, TAalphaGTase, TLGT, TmalphaGT, TreX, TS alpha GTase, TSalphaGT

ECTree

     2 Transferases

         2.4 Glycosyltransferases

             2.4.1 Hexosyltransferases

                2.4.1.25 4-alpha-glucanotransferase

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Results	in table
11	Activating Compound
28695	AA Sequence
23	Application
1	CAS Registry Number
79	Cloned(Commentary)
13	Crystallization (Commentary)
38	Disease/ Diagnostics
1	Expression
2	General Information
2	General Stability
29	Inhibitors
25	kcat/KM [mM/s]
3	Ki Value [mM]
52	KM Value [mM]
16	Localization
1	Metals/Ions
61	Molecular Weight [Da]
28	Natural Substrates/ Products (Substrates)
476	Organism
11	Pathway
63	PDB ID
50	pH Optimum
7	pH Range
9	pH Stability
3	pI Value
1	Posttranslational Modification
94	Protein Variants
59	Purification (Commentary)
3	Reaction
3	Reaction Type
229	Reference
60	Source Tissue
19	Specific Activity [micromol/min/mg]
9	Storage Stability
464	Substrates and Products (Substrate)
54	Subunits
155	Synonyms
1	Systematic Name
51	Temperature Optimum [°C]
7	Temperature Range [°C]
32	Temperature Stability [°C]
53	Turnover Number [1/s]

Storage Stability

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Storage Stability on EC 2.4.1.25 - 4-alpha-glucanotransferase

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STORAGE STABILITY

ORGANISM

UNIPROT

LITERATURE

-20°C, stored in purification buffer, activity is stable for over 6 months

Chlamydomonas reinhardtii

-

489014

-80°C, purified recombinant enzyme, 20 mM sodium phosphate buffer containing 15% glycerol, pH 7.0, no decrease in acivity observed for 1 month

Saccharomyces cerevisiae

-

489018

0°C, unstable to freezing

Pisum sativum

-

489001

4°C, purified enzyme in buffer shows little or no loss of activity in 1 month

Oryctolagus cuniculus

-

171387

4°C, relatively stable at low temperatures, may be stored for many months in a cold room with relatively little loss of activity

Bacillus sp. (in: Bacteria)

-

488995

4°C, stable for several months

Pisum sativum

-

489001

4°C, stable in 20 mM bis-Tris-propane buffer, pH 6.5, containing 10% glycerol, no loss of activity over 6 months

Arabidopsis thaliana

-

489000

4°C, stored as a suspension in 3.2 M ammonium sulfate, no significant loss of activity for at least 1 month

Pseudomonas stutzeri

-

488998

4°C, very stable over long periods

Arabidopsis thaliana

-

489001