2.4.1.254: cyanidin-3-O-glucoside 2''-O-glucuronosyltransferase
This is an abbreviated version!
For detailed information about cyanidin-3-O-glucoside 2''-O-glucuronosyltransferase, go to the full flat file.
Reaction
Synonyms
BpUGAT, BpUGT94B1, UDP-glucuronic acid:anthocyanidin 3-glucoside 2'-O-beta-glucuronosyltransferase, UDP-glucuronic acid:anthocyanin glucuronosyltransferase
ECTree
2.4.1.254 cyanidin-3-O-glucoside 2''-O-glucuronosyltransferaseAdvanced search results
results (
results (Substrates Products
Substrates Products on EC 2.4.1.254 - cyanidin-3-O-glucoside 2''-O-glucuronosyltransferase
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SUBSTRATE 
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
UDP-beta-D-glucuronate + cyanidin 3-O-beta-D-glucoside
UDP + cyanidin 3-O-beta-(2-O-beta-D-glucuronosyl)-beta-D-glucoside
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highly specific with respect to the sugar donor UDP-GlcUA. Activity with other activated sugars such as UDP-Glc, UDP-Gal, and UDP-Xyl is very low. Wild-type enzyme activity toward delphinidin-3-O-glucoside is only 5% to 10% of the activity with cyanidin 3-O-beta-D-glucoside. A few specific amino acid residues as well as the overall size and shape of the acceptor pocket define substrate specificity
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?
UDP-D-glucuronate + cyanidin 3-O-beta-D-glucoside
UDP + cyanidin 3-O-(2-O-beta-D-glucuronosyl)-beta-D-glucoside
UDP-D-glucuronate + cyanidin-3-O-(6-O-malonyl-beta-D-glucoside)
UDP + cyanidin 3-O-(2-O-beta-D-glucuronosyl-6-O-malonyl-beta-D-glucoside)
UDP-D-glucuronate + cyanidin 3-O-beta-D-glucoside
UDP + cyanidin 3-O-(2-O-beta-D-glucuronosyl)-beta-D-glucoside
both cyanidin-3-O-6''-malonylglucoside and cyanidin 3-O-glucoside are good substrates, suggesting that these anthocyanins may serve as physiological glucuronosyl acceptors in the production of glucuronosylated anthocyanins that are the origin of the red coloration of flowers of Bellis perennis
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?
UDP-D-glucuronate + cyanidin 3-O-beta-D-glucoside
UDP + cyanidin 3-O-(2-O-beta-D-glucuronosyl)-beta-D-glucoside
50% of the activity with cyanidin-3-O-glucoside. The enzyme is highly specific for cyanidin 3-O-glucosides and UDP-D-glucuronate. The relative activities for UDP-D-glucose and UDP-D-galactose are less than 0.1% of the activity for UDP-D-glucuronate. The enzyme shows low activity with delphinidin 3-O-glucoside and is shows no or negligible activity towards pelargonidin 3-O-glucoside,cyanidin 3-O-3'',6''-O-dimalonylglucoside, pelargonidin 3,5-O-diglucoside, pelargonidin 3-O-6''-O-malonylglucoside-5-O-glucoside, quercetin 3-O-glucoside, quercetin 3-O-6''-O-malonylglucoside, daidzin, genistin, daidzin, genistin, 7-O-6''-O-malonylglucosides of daidzein and genistein, cyanidin, quercetin, daidzein, genistein, p-nitrophenyl beta-D-glucopyranoside
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?
UDP-D-glucuronate + cyanidin-3-O-(6-O-malonyl-beta-D-glucoside)
UDP + cyanidin 3-O-(2-O-beta-D-glucuronosyl-6-O-malonyl-beta-D-glucoside)
both cyanidin-3-O-(6'-O-malonyl-beta-D-glucoside) and cyanidin 3-O-glucoside are good substrates, suggesting that these anthocyanins may serve as physiological glucuronosyl acceptors in the production of glucuronosylated anthocyanins that are the origin of the red coloration of flowers of Bellis perennis
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?
UDP-D-glucuronate + cyanidin-3-O-(6-O-malonyl-beta-D-glucoside)
UDP + cyanidin 3-O-(2-O-beta-D-glucuronosyl-6-O-malonyl-beta-D-glucoside)
50% of the activity with cyanidin-3-O-glucoside. The enzyme is highly specific for cyanidin 3-O-glucosides and UDP-D-glucuronate. The relative activities for UDP-D-glucose and UDP-D-galactose are less than 0.1% of the activity for UDP-D-glucuronate. The enzyme shows low activity with delphinidin 3-O-glucoside and it shows no or negligible activity towards pelargonidin 3-O-glucoside,cyanidin 3-O-3'',6''-O-dimalonylglucoside, pelargonidin 3,5-O-diglucoside, pelargonidin 3-O-6''-O-malonylglucoside-5-O-glucoside, quercetin 3-O-glucoside, quercetin 3-O-6''-O-malonylglucoside, daidzin, genistin, daidzin, genistin, 7-O-6''-O-malonylglucosides of daidzein and genistein, cyanidin, quercetin, daidzein, genistein, p-nitrophenyl beta-D-glucopyranoside
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