2.4.1.255: protein O-GlcNAc transferase This is an abbreviated version! For detailed information about protein O-GlcNAc transferase, go to the full flat file .
Word Map on EC 2.4.1.255
Reaction
UDP-N-acetyl-alpha-D-glucosamine +
[protein]-L-serine =
UDP +
[protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-serine
Synonyms beta-N-acetylglucosaminidase, EGF domain-specific O-GlcNAc transferase, EGF domain-specific O-linked N-acetylglucosamine transferase, EOGT, EOGT1, epidermal growth factor domain-specific O-GlcNAc transferase, glycosyltransferase OGT, Gtf1, GtfA, hOGT, HsOGT, human OGT, mammalian OGT, mOGT, More, N-acetylglucosamine transferase, N-acetylglucosamine-peptide N-acetylglucosaminyltransferase, N-acetylglucosaminyltransferase, ncOGT, nuclear cytoplasmic OGT, nucleocytoplasmic glycosyltransferase, nucleocytoplasmic OGT, O-GlcNAc protein, O-GlcNAc transferase, O-GlcNAc transferase OGT, O-GlcNAc-transferase, O-GlcNAcase, O-glycosylated protein p135, O-linked beta-N-acetylglucosamine transferase, O-linked beta-N-acetylglucosaminyltransferase, O-linked GlcNAc transferase, O-linked N-acetylglucosamine transferase, O-linked N-acetylglucosaminyltransferase, O-N-acetylglucosamine (O-GlcNAc) transferase, OGT, OGT p110, OGT protein, ogta, OGTase, OGTase protein, ogtb, p135, Protein SECRET AGENT, SEC, SECRET AGENT, sOGT, spindly, SPY, super sex combs, Sxc, Synpcc7942_0051, transferase OGT, UDP-GlcNAc: polypeptide O-N-acetylglucosaminyltransferase, UDP-GlcNAc:polypeptidyl transferase, UDP-N-acetylglucosaminyl transferase, uridine diphosphate-N-acetyl-D-glucosamine:polypeptidyltransferase, uridine diphospho-N-acetylglucosamine:polypeptide beta-N-acetylaminyltransferase, uridine diphospho-N-acetylglucosamine:polypeptide beta-N-acetylglucosaminyltransferase, XcOGT, XcOGT protein
ECTree
KM Value
KM Value on EC 2.4.1.255 - protein O-GlcNAc transferase
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0.0012
Nup 62 protein
-
-
-
0.000025 - 0.000227
Nup62
-
0.00335
OIP106 protein
-
apparent Km of OGT for the OID of OIP106 protein
-
0.107 - 0.215
PGGSTPVSSANMM
0.3695
UDP-4-deoxy-GalNAc
pH 7.4, 37°C
0.1418
UDP-6-deoxy-GlcNAc
pH 7.4, 37°C
0.2821
UDP-GlcNPr
pH 7.4, 37°C
0.1757 - 0.1805
UDP-N-acetyl-alpha-D-glucosamine
0.0047 - 0.0085
UDP-N-azidoacetylglucosamine
additional information
additional information
-
0.000025
Nup62
-
-
-
0.107
PGGSTPVSSANMM
-
His-p110 trimer
0.215
PGGSTPVSSANMM
-
D6 monomer
0.0005
UDP-GlcNAc
-
-
0.000545
UDP-GlcNAc
-
extremely high affinity
0.0021
UDP-GlcNAc
-
wild-type, pH 7.2 22°C
0.0022
UDP-GlcNAc
using calcium/calmodulin-dependent kinase IV as cosubstrate, at 37°C, pH not specified in the publication
0.0047
UDP-GlcNAc
using CARM1 protein as cosubstrate, at 37°C, pH not specified in the publication
0.0058
UDP-GlcNAc
using Nup62 protein as cosubstrate, at 37°C, pH not specified in the publication
0.006
UDP-GlcNAc
-
calculated from the linear regions of the reciprocal plot from 0.05 mM to 4.8 mM UDP-GlcNAc, His-p110 trimer
0.006
UDP-GlcNAc
-
D6 monomer
0.007
UDP-GlcNAc
using TAB1 protein as cosubstrate, at 37°C, pH not specified in the publication
0.0085
UDP-GlcNAc
pH 7.4, 37°C
0.0155
UDP-GlcNAc
-
mutant C917A, pH 7.2 22°C
0.0178
UDP-GlcNAc
pH 7.5, temperature not specified in the publication
0.035
UDP-GlcNAc
-
calculated from the linear regions of the reciprocal plot from 0.05 mM to 4.8 mM UDP-GlcNAc, His-p110 trimer
0.06
UDP-GlcNAc
-
D6 monomer
0.15
UDP-GlcNAc
-
F752A mutant
0.217
UDP-GlcNAc
-
calculated from the linear regions of the reciprocal plot from 0.05 mM to 4.8 mM UDP-GlcNAc, His-p110 trimer
0.29
UDP-GlcNAc
-
D422A mutant
0.44
UDP-GlcNAc
-
F776A mutant
0.8
UDP-GlcNAc
-
mOGT wild type
1.1
UDP-GlcNAc
GtfA-GtfB complex, pH 7.5, 37°C
1.9
UDP-GlcNAc
-
Y434A mutant, approximately double the Km value of wild type
11.8
UDP-GlcNAc
isolated core enzyme GtfA, pH 7.5, 37°C
0.1757
UDP-N-acetyl-alpha-D-glucosamine
37°C, pH not specified in the publication, enzyme form without O-GlcNAcylation
0.1805
UDP-N-acetyl-alpha-D-glucosamine
37°C, pH not specified in the publication, O-GlcNAcylated enzyme
0.0047
UDP-N-azidoacetylglucosamine
-
wild-type, pH 7.2 22°C
0.0085
UDP-N-azidoacetylglucosamine
-
mutant C917A, pH 7.2 22°C
additional information
additional information
-
kinetic studies suggest that OGT functions through a random bi-bi kinetic mechanism and that the protein has three distinct binding constants for the UDPGlcNAc sugar donor
-
additional information
additional information
-
kinetic experiments using recombinant hOGT/XcOGT
-
additional information
additional information
-
kinetic experiments using recombinant hOGT/XcOGT
-