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2.4.1.257: GDP-Man:Man2GlcNAc2-PP-dolichol alpha-1,6-mannosyltransferase

This is an abbreviated version!
For detailed information about GDP-Man:Man2GlcNAc2-PP-dolichol alpha-1,6-mannosyltransferase, go to the full flat file.

Reaction

GDP-alpha-D-mannose
+
alpha-D-Man-(1->3)-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-alpha-D-GlcNAc-diphosphodolichol
=
GDP
+
alpha-D-Man-(1->3)-[alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-alpha-D-GlcNAc-diphosphodolichol

Synonyms

Alg2, Alg2 mannosyltransferase, Alg2 MTase, More, MTase, scAlg2

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.257 GDP-Man:Man2GlcNAc2-PP-dolichol alpha-1,6-mannosyltransferase

Purification

Purification on EC 2.4.1.257 - GDP-Man:Man2GlcNAc2-PP-dolichol alpha-1,6-mannosyltransferase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant N-terminal thioredoxin (Trx)-His6-tagged wild-type and mutant ALG2s from Escherichia coli strain DE3 membranes, preparation of proteoliposomes
recombinant N-terminally His10-YFP tagged enzyme from HEK-293 cells by solubilization from membranes with detergent, nickel affinity chromatography, and gel filtration