2.4.1.258: dolichyl-P-Man:Man5GlcNAc2-PP-dolichol alpha-1,3-mannosyltransferase
This is an abbreviated version!
For detailed information about dolichyl-P-Man:Man5GlcNAc2-PP-dolichol alpha-1,3-mannosyltransferase, go to the full flat file.
Word Map on EC 2.4.1.258
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2.4.1.258
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n-glycosylation
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n-glycans
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lipid-linked
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oligosaccharide
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glc3man9glcnac2
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glycan
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medicine
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peptide-n-glycosidase
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synthesis
- 2.4.1.258
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n-glycosylation
- n-glycans
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lipid-linked
- oligosaccharide
- glc3man9glcnac2
- glycan
- medicine
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peptide-n-glycosidase
- synthesis
Reaction
Synonyms
Alg3, Alg3p, AtALG3, Dol-P-Man:Man5GlcNAc2-PP-Dol alpha1,3-mannosyl transferase, Dol-P-Man:Man5GlcNAc2-PP-Dol mannosyltransferase, dolichyl-P-Man:Man(5)GlcNAc(2)-PP-dolichyl mannosyltransferase, EC 2.4.1.130, Not56-like protein, PpAlg3 alpha-1,3-mannosyl transferase, TbALG3
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Substrates Products
Substrates Products on EC 2.4.1.258 - dolichyl-P-Man:Man5GlcNAc2-PP-dolichol alpha-1,3-mannosyltransferase
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REACTION DIAGRAM
dolichyl beta-D-mannosyl phosphate + D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->6)]-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol
D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->3)-D-Man-alpha-(1->6)]-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol + dolichyl phosphate
GDP-alpha-D-mannose + Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)(Manalpha(1-6))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-PP-dolichol
GDP + Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)(Manalpha(1-3)Manalpha(1-6))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-PP-dolichol
dolichyl beta-D-mannosyl phosphate + D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->6)]-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol
D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->3)-D-Man-alpha-(1->6)]-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol + dolichyl phosphate
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dolichyl beta-D-mannosyl phosphate + D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->6)]-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol
D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->3)-D-Man-alpha-(1->6)]-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol + dolichyl phosphate
the core oligosaccharide Glc3Man9GlcNAc2 is assembled by a series of membrane-bound glycosyltransferases as the lipid carrier dolichylpyrophosphate-linked glycan in the endoplasmic reticulum (ER). The first step of this assembly pathway on the ER luminal side is mediated by ALG3. AtALG3 is a critical factor for mature N-glycosylation of proteins, but not essential for cell viability and growth in Arabidopsis
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dolichyl beta-D-mannosyl phosphate + D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->6)]-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol
D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->3)-D-Man-alpha-(1->6)]-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol + dolichyl phosphate
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dolichyl beta-D-mannosyl phosphate + D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->6)]-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol
D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->3)-D-Man-alpha-(1->6)]-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol + dolichyl phosphate
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dolichyl beta-D-mannosyl phosphate + D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->6)]-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol
D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->3)-D-Man-alpha-(1->6)]-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol + dolichyl phosphate
regulatory role for the Alg3p-dependent alpha1,3-linked Man in subsequent oligosaccharide-lipid and glycoprotein glycan maturation. The presence of this Man appears to provide structural information potentiating the downstream action of the endoplasmic reticulum glucosyltransferases Alg6p, Alg8p and Alg10p, glucosidases Gls1p and Gls2p, and the Golgi Och1p outerchain alpha1,6-Man branch-initiating mannosyltransferase
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dolichyl beta-D-mannosyl phosphate + D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->6)]-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol
D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->3)-D-Man-alpha-(1->6)]-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol + dolichyl phosphate
the formation of N-glycosidic linkages of glycoproteins involves the ordered assembly of the common Glc3Man9GlcNAc2 core-oligosaccharide on the lipid carrier dolichyl diphosphate. Early mannosylation steps occur on the cytoplasmic side of the endoplasmic reticulum with GDP-Man as donor, the final reactions from Man5GlcNAc2-PP-Dol to Man9Glc-NAc2-PP-Dol on the lumenal side use dolichyl beta-D-mannosyl phosphate. Alg3p is not required for elongation of D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->3)-D-Man-alpha-(1->6)]-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol
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dolichyl beta-D-mannosyl phosphate + D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->6)]-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol
D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->3)-D-Man-alpha-(1->6)]-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol + dolichyl phosphate
extension does not occur when the acceptor was added as a free Man5GlcNAc2 oligosaccharide or when GDP-Man is used as mannosyl donor
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GDP-alpha-D-mannose + Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)(Manalpha(1-6))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-PP-dolichol
GDP + Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)(Manalpha(1-3)Manalpha(1-6))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-PP-dolichol
alg3 Man5GlcNAc2 precursor
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GDP-alpha-D-mannose + Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)(Manalpha(1-6))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-PP-dolichol
GDP + Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)(Manalpha(1-3)Manalpha(1-6))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-PP-dolichol
cytosolic alg3 Man5GlcNAc2 precursor
product of the Alg3p step
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GDP-alpha-D-mannose + Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)(Manalpha(1-6))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-PP-dolichol
GDP + Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)(Manalpha(1-3)Manalpha(1-6))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-PP-dolichol
cytosolic alg3 Man5GlcNAc2 precursor
product of the Alg3p step
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GDP-alpha-D-mannose + Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)(Manalpha(1-6))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-PP-dolichol
GDP + Manalpha(1-2)Manalpha(1-2)Manalpha(1-3)(Manalpha(1-3)Manalpha(1-6))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-PP-dolichol
alg3 Man5GlcNAc2 precursor
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Alg3-mediated N-glycosylation of the effector, Secreted LysM Protein1 (Slp1), is essential for its activity. Slp1 has three N-glycosylation sites and simultaneous Alg3-mediated N-glycosylation of each site is required to maintain protein stability and the chitin binding activity of Slp1
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additional information
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MNN1 encoded enzyme adds at least the penultimate alpha-1,3-linked Man of the terminal Manalpha(1-3)Manalpha(1-3)-disaccharide on O-linked glycans
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additional information
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MNN1 encoded enzyme adds at least the penultimate alpha-1,3-linked Man of the terminal Manalpha(1-3)Manalpha(1-3)-disaccharide on O-linked glycans
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additional information
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regulatory role for the Alg3p-dependent alpha-1,3-linked Man in subsequent oligosaccharide-lipid and glycoprotein glycan maturation, it provides structural information that potentiates the Alg6p, Alg8p and Alg10p ER glucosyltransferases and Gls1p and Gls2p trimming glucosidases
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additional information
?
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regulatory role for the Alg3p-dependent alpha-1,3-linked Man in subsequent oligosaccharide-lipid and glycoprotein glycan maturation, it provides structural information that potentiates the Alg6p, Alg8p and Alg10p ER glucosyltransferases and Gls1p and Gls2p trimming glucosidases
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additional information
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involved in oligosaccharide-lipid synthesis
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additional information
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MNN1 encoded enzyme adds at least the penultimate alpha-1,3-linked Man of the terminal Manalpha(1-3)Manalpha(1-3)-disaccharide on O-linked glycans
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-
?
additional information
?
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regulatory role for the Alg3p-dependent alpha-1,3-linked Man in subsequent oligosaccharide-lipid and glycoprotein glycan maturation, it provides structural information that potentiates the Alg6p, Alg8p and Alg10p ER glucosyltransferases and Gls1p and Gls2p trimming glucosidases
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additional information
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involved in oligosaccharide-lipid synthesis
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?