2.4.1.26: DNA alpha-glucosyltransferase

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For detailed information about DNA alpha-glucosyltransferase, go to the full flat file.

Reaction

Synonyms

AGT, glucosyltransferase, uridine diphosphoglucose-deoxyribonucleate alpha-, T2-HMC-alpha-glucosyl transferase, T4-HMC-alpha-glucosyl transferase, T6-HMC-alpha-glucosyl transferase, UDPglucose-DNA alpha-glucosyltransferase, uridine diphosphoglucose-deoxyribonucleate alpha-glucosyltransferase

ECTree

     2 Transferases

         2.4 Glycosyltransferases

             2.4.1 Hexosyltransferases

                2.4.1.26 DNA alpha-glucosyltransferase

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Results	in table
2	Activating Compound
2	AA Sequence
1	CAS Registry Number
2	Cloned(Commentary)
2	Metals/Ions
1	Molecular Weight [Da]
6	Organism
9	PDB ID
1	pH Optimum
6	Protein Variants
5	Purification (Commentary)
2	Reaction
1	Reaction Type
6	Reference
4	Specific Activity [micromol/min/mg]
7	Substrates and Products (Substrate)
2	Subunits
7	Synonyms
1	Systematic Name
1	Temperature Optimum [°C]

Engineering

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Engineering on EC 2.4.1.26 - DNA alpha-glucosyltransferase

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PROTEIN VARIANTS

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

E22A

Tequatrovirus T4

-

site-directed mutagenesis, 50% activity compared to the wild-type enzyme, in absence of acceptor 70% reduced UDG-glucose turnover compared to the wild-type enzyme

657775

E311A

Tequatrovirus T4

-

site-directed mutagenesis, 1-2% activity compared to the wild-type enzyme, in absence of acceptor no remaining UDG-glucose turnover

657775

M231A

Tequatrovirus T4

-

site-directed mutagenesis, 1-2% activity compared to the wild-type enzyme, in absence of acceptor mutant shows an unaltered UDG-glucose turnover compared to the wild-type enzyme

657775

R236A

Tequatrovirus T4

-

site-directed mutagenesis, 27% activity compared to the wild-type enzyme, in absence of acceptor mutant shows an unaltered UDG-glucose turnover compared to the wild-type enzyme

657775

R256A

Tequatrovirus T4

-

site-directed mutagenesis, activity is similar to the wild-type enzyme, in absence of acceptor mutant shows an unaltered UDG-glucose turnover compared to the wild-type enzyme

657775

S106A

Tequatrovirus T4

-

site-directed mutagenesis, 111% activity compared to the wild-type enzyme, in absence of acceptor mutant shows an unaltered UDG-glucose turnover compared to the wild-type enzyme

657775