2.4.1.264: D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphosphoundecaprenol 2-beta-glucuronosyltransferase

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For detailed information about D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphosphoundecaprenol 2-beta-glucuronosyltransferase, go to the full flat file.

Reaction

Synonyms

gumK

ECTree

     2 Transferases

         2.4 Glycosyltransferases

             2.4.1 Hexosyltransferases

                2.4.1.264 D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphosphoundecaprenol 2-beta-glucuronosyltransferase

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Results	in table
46	AA Sequence
2	Application
2	Cloned(Commentary)
3	Crystallization (Commentary)
7	General Information
10	kcat/KM [mM/s]
20	KM Value [mM]
2	Localization
1	Molecular Weight [Da]
4	Natural Substrates/ Products (Substrates)
8	Organism
2	Pathway
2	pH Optimum
12	Protein Variants
3	Purification (Commentary)
1	Reaction
8	Reference
12	Substrates and Products (Substrate)
1	Subunits
4	Synonyms
1	Systematic Name
2	Temperature Optimum [°C]
10	Turnover Number [1/s]

Crystallization

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Crystallization on EC 2.4.1.264 - D-Man-alpha-(1->3)-D-Glc-beta-(1->4)-D-Glc-alpha-1-diphosphoundecaprenol 2-beta-glucuronosyltransferase

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CRYSTALLIZATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

hanging drop vapour diffusion method, crystal structure of apo-enzyme at 1.9 A resolution, and of enzyme in complex with UDP, at 2.28 A resolution. Residue Asp157 serves as the general base in the transfer reaction. Residues M231, M273, E272, Y292, M306, K307, and Q310 interact with UDP. Cocrystallisation of GumK or mutant D157A in presence of UDP-glucuronate is not possible

Xanthomonas campestris

Q8GCH2

704434

hanging-drop vapour diffusion method, crystals of recombinant GumK, 1.9 A resolution

Xanthomonas campestris

-

701482

in silico-built models of GumK complexed with UDP-GlcA as well as its analogs UDP-glucose and UDP-galacturonic acid. UDP-GlcA binding to GumK triggers a change in the GumK tertiary structure, affecting N-terminal hydrophobic residues to generate or improve the lipid-derived acceptor substrate site, while UDP-GlcA accommodates the transfer reaction

Xanthomonas campestris

Q8GCH2

737190