2.4.1.265: dolichyl-P-Glc:Glc1Man9GlcNAc2-PP-dolichol alpha-1,3-glucosyltransferase
This is an abbreviated version!
For detailed information about dolichyl-P-Glc:Glc1Man9GlcNAc2-PP-dolichol alpha-1,3-glucosyltransferase, go to the full flat file.
Reaction
Synonyms
ALG8, Dol-P-Glc:Glc1-Man9-GlcNAc2-P-P-Dol glucosyltransferase, dolichyl-P-Glc:Glc1 Man9GlcNAc2-PP-dolichyl alpha1,3-glucosyltransferase, dolichyl-P-Glc:Glc1Man9GlcNAc2-PP-dolichyl alpha1,3-glucosyltransferase, dolichyl-P-glucose:Glc1Man9GlcNAc2-PP-dolichyl alpha3-glucosyltransferase, hALG8, hALG8p
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Application
Application on EC 2.4.1.265 - dolichyl-P-Glc:Glc1Man9GlcNAc2-PP-dolichol alpha-1,3-glucosyltransferase
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synthesis
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expression of the genes encoding for alginate-lyase algL and the catalytic subunit of the alginate polymerase complex, dolichyl-P-Glc:Glc1Man9GlcNAc2-PP-dolichol alpha-1,3-glucosyltransferase alg8, in chemostat cultures of Azotobacter vinelandii ATCC 9046. Increased alginate production of 2.4 g per l and a higher specific alginate production rate of 0.1 g per g and h are obtained at an inlet sucrose concentration of 15 g per l. Carbon recovery of about 100% is obtained at an inlet sucrose concentration of 10 g per l, whereas it is close to 50% at higher inlet sucrose concentrations. An increase in the molecular weight of alginate is linked to higher alg8 gene expression