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2.4.1.277: 10-deoxymethynolide desosaminyltransferase

This is an abbreviated version!
For detailed information about 10-deoxymethynolide desosaminyltransferase, go to the full flat file.

Word Map on EC 2.4.1.277

Reaction

dTDP-3-dimethylamino-3,4,6-trideoxy-alpha-D-glucopyranose
+
10-deoxymethynolide
=
dTDP
+
10-deoxymethymycin

Synonyms

DesVII, DesVII/DesVIII, glycosyltransferase DesVII, macrolide glycosyltransferase DesVII

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.277 10-deoxymethynolide desosaminyltransferase

Cofactor

Cofactor on EC 2.4.1.277 - 10-deoxymethynolide desosaminyltransferase

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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
DesIII protein
glycosyltransferase DesVII is unique among glycosyltransferases in that it requires an additional protein component, DesVIII, for activity. desVIII is important for the biosynthesis of glycosylated macrolides but can be replaced by at least one of the homologous genes from other pathways. The addition of recombinant DesVIII protein significantly improves the glycosylation efficiency of DesVII in the in vitro assay. DesVIII assists the folding of DesVII during protein production and remains tightly bound during catalysis. Although the formation of the DesVII/Des-VIII complex is essential for the catalytic activity, DesVIII is unlikely involved in catalysis directly
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DesVIII protein
DesVIII is an auxiliary protein which enhances the transfer of TDP-D-desosamine catalyzed by DesVII glycosyltransferase in the biosynthesis of macrolide antibiotics, neomethymycin, methymycin and pikromycin, in Streptomyces venezuelae ATCC 15439. The DesVIII homologues (except for TylMIII) can functionally replace the native DesVIII for glycosylation to proceed in vivo and their functions are similar in acting as glycosyltransferase auxiliary proteins. Complementation by expressing EryCII, OleP1, and DnrQ in a desVIII deletion mutant strain restors the production of glycosylated macrolides to an approximate level of 66%, 26% and 26%, respectively, compared to self-complementation by DesVIII
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