2.4.1.292: GalNAc-alpha-(1->4)-GalNAc-alpha-(1->3)-diNAcBac-PP-undecaprenol alpha-1,4-N-acetyl-D-galactosaminyltransferase
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For detailed information about GalNAc-alpha-(1->4)-GalNAc-alpha-(1->3)-diNAcBac-PP-undecaprenol alpha-1,4-N-acetyl-D-galactosaminyltransferase, go to the full flat file.
Reaction
3 UDP-N-acetyl-alpha-D-galactosamine + = 3 UDP +
Synonyms
PglH, pglH2
ECTree
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Purification
Purification on EC 2.4.1.292 - GalNAc-alpha-(1->4)-GalNAc-alpha-(1->3)-diNAcBac-PP-undecaprenol alpha-1,4-N-acetyl-D-galactosaminyltransferase
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recombinant His-tagged PglH from Escherichia coli by nickel affinity chromatography
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recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21-Gold (DE3) by ultracentrifugation, nickel affinity chromatography, desalting gel filtration, gel filtration, and ultratfiltration
recombinant N-terminally octahistidine-tagged enzyme from Escherichia coli strain BL-21 RIL by nickel affinity chromatography, followed by cleavage of the His8-tag by TEV protease and another step of nickel affinity chromatography
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