2.4.1.304: UDP-Gal:alpha-D-GlcNAc-diphosphoundecaprenol beta-1,4-galactosyltransferase

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For detailed information about UDP-Gal:alpha-D-GlcNAc-diphosphoundecaprenol beta-1,4-galactosyltransferase, go to the full flat file.

Reaction

Synonyms

UDP-Gal:GlcNAc-pyrophosphate-lipid beta-1,4-galactosyltransferase, UDP-Gal:GlcNAc-R 1,4-Gal-transferase, WfeD

ECTree

     2 Transferases

         2.4 Glycosyltransferases

             2.4.1 Hexosyltransferases

                2.4.1.304 UDP-Gal:alpha-D-GlcNAc-diphosphoundecaprenol beta-1,4-galactosyltransferase

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Results	in table
2	Activating Compound
20	AA Sequence
1	Cloned(Commentary)
4	Inhibitors
2	KM Value [mM]
5	Metals/Ions
1	Molecular Weight [Da]
2	Natural Substrates/ Products (Substrates)
2	Organism
1	pH Optimum
11	Protein Variants
1	Purification (Commentary)
1	Reaction
2	Reference
1	Storage Stability
12	Substrates and Products (Substrate)
2	Subunits
6	Synonyms
1	Systematic Name
1	Temperature Optimum [°C]

Engineering

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Engineering on EC 2.4.1.304 - UDP-Gal:alpha-D-GlcNAc-diphosphoundecaprenol beta-1,4-galactosyltransferase

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PROTEIN VARIANTS

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

D99A

Shigella boydii

-

highly active mutant enzyme

722531

E101A

Shigella boydii

-

mutant exhibits minimal enzyme activity, greatly reduced enzyme activity in the presence of either Mn2+ or Pb2+

722531

E103A

Shigella boydii

-

highly active mutant enzyme

722531

K211A

Shigella boydii

-

greatly reduced activity, 60fold higher activity in the presence of Pb2+ than in the presence of Mn2+

722531

R210A

Shigella boydii

-

active mutant enzyme

722531

R212A

Shigella boydii

-

active mutant enzyme

722531

D99A

Shigella boydii B14

-

highly active mutant enzyme

-

E101A

Shigella boydii B14

-

mutant exhibits minimal enzyme activity, greatly reduced enzyme activity in the presence of either Mn2+ or Pb2+

-

E103A

Shigella boydii B14

-

highly active mutant enzyme

-

R210A

Shigella boydii B14

-

active mutant enzyme

-

R212A

Shigella boydii B14

-

active mutant enzyme

-