2.4.1.31: laminaribiose phosphorylase
This is an abbreviated version!
For detailed information about laminaribiose phosphorylase, go to the full flat file.
Word Map on EC 2.4.1.31
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2.4.1.31
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euglena
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phosphorylases
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gracilis
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glycoside
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1-phosphate
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phosphorolysis
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polymerization
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bienzymatic
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oligosaccharide
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sepabeads
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biocatalysts
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glucan
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cellobiose
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laminaritriose
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epoxy
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gps
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paenibacillus
- 2.4.1.31
-
euglena
- phosphorylases
- gracilis
- glycoside
- 1-phosphate
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phosphorolysis
- polymerization
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bienzymatic
- oligosaccharide
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sepabeads
-
biocatalysts
- glucan
- cellobiose
- laminaritriose
-
epoxy
- gps
- paenibacillus
Reaction
Synonyms
1,3-beta-D-glucobiose, ACL0729, GH94_B, GH94_C, glucose-beta-(1->3)-glucose phosphorylase, laminaribiose phosphorylase, LBP, LBPbac, Pro_7066
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Substrates Products
Substrates Products on EC 2.4.1.31 - laminaribiose phosphorylase
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REACTION DIAGRAM
alpha-D-glucose 1-phosphate + 1,5-anhydro-D-glucitol
3-O-beta-D-glucopyranosyl-1,5-anhydro-D-glucitol + phosphate
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-
-
r
alpha-D-glucose 1-phosphate + 2-deoxy-D-arabino-hexopyranose
?
-
-
-
r
alpha-D-glucose 1-phosphate + D-glucose
laminaribiose + laminaritriose + laminaritetraose + laminaripentaose + phosphate
alpha-D-glucose 1-phosphate + D-glucuronic acid
3-O-beta-D-glucopyranosyl-D-glucuronic acid + phosphate
-
-
-
r
alpha-D-glucose 1-phosphate + D-mannose
3-O-beta-D-glucopyranosyl-D-mannose + phosphate
-
-
-
r
alpha-D-glucose 1-phosphate + D-xylose
?
the major product (83%) in the reaction with D-xylose is 3-O-beta-D-glucopyranosyl-D-xylose and the other fraction (17%) contains 2-O-beta-D-glucopyranosyl-D-xylose as the major component
-
-
r
alpha-D-mannose 1-phosphate + D-glucose
beta-D-mannopyranosyl-(1->3)-D-glucopyranose + phosphate
-
-
-
r
D-glucose + alpha-D-glucose 1-phosphate
3-beta-D-glucosyl-D-glucose + phosphate
-
-
-
-
?
D-glucose + alpha-D-glucose 1-phosphate
Astasia ocellata
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the enzyme is specific for the alpha-isomer
-
r
3-beta-D-glucosyl-D-glucose + phosphate
D-glucose + alpha-D-glucose 1-phosphate
-
-
-
?
3-beta-D-glucosyl-D-glucose + phosphate
D-glucose + alpha-D-glucose 1-phosphate
-
-
-
r
3-beta-D-glucosyl-D-glucose + phosphate
D-glucose + alpha-D-glucose 1-phosphate
-
-
-
r
phosphate + oligosaccharides
Astasia ocellata
-
-
oligosaccharides with two or more 1,3-linked glucose residues attached to the nonreducing residue of cellobiose
r
alpha-D-glucose 1-phosphate + cellobiose
phosphate + oligosaccharides
-
-
-
-
r
laminaribiose + laminaritriose + laminaritetraose + laminaripentaose + phosphate
Astasia ocellata
-
at equilibrium synthesis of laminaribiose is favoured
-
r
alpha-D-glucose 1-phosphate + D-glucose
laminaribiose + laminaritriose + laminaritetraose + laminaripentaose + phosphate
Astasia ocellata
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specific for alpha-D-glucose 1-phosphate as glucosyl donor
-
r
alpha-D-glucose 1-phosphate + D-glucose
laminaribiose + laminaritriose + laminaritetraose + laminaripentaose + phosphate
-
-
-
?
alpha-D-glucose 1-phosphate + D-glucose
laminaribiose + laminaritriose + laminaritetraose + laminaripentaose + phosphate
-
initial rate of synthetic reaction is 4 times faster than that of phosphorolytic reaction
-
r
alpha-D-glucose 1-phosphate + D-glucose
laminaribiose + laminaritriose + laminaritetraose + laminaripentaose + phosphate
-
specific for alpha-D-glucose 1-phosphate as glucosyl donor
amount of individual products depends on substrate concentration and reaction time
?
laminaribiose + phosphate
-
-
-
r
alpha-D-glucose 1-phosphate + D-glucose
laminaribiose + phosphate
-
-
-
-
?
alpha-D-glucose 1-phosphate + D-glucose
laminaribiose + phosphate
-
-
-
-
?
alpha-D-glucose 1-phosphate + D-glucose
laminaribiose + phosphate
-
-
-
r
alpha-D-glucose 1-phosphate + D-glucose
laminaribiose + phosphate
-
-
-
-
r
laminaritriose + phosphate
-
-
-
-
?
laminaribiose + alpha-D-glucose 1-phosphate
laminaritriose + phosphate
-
-
-
-
?
alpha-D-glucose 1-phosphate + D-glucose
-
-
-
r
laminaribiose + phosphate
alpha-D-glucose 1-phosphate + D-glucose
-
-
-
r
laminaribiose + phosphate
alpha-D-glucose 1-phosphate + D-glucose
-
-
-
-
r
laminaritetraose + alpha-D-glucose 1-phosphate
-
-
-
?
laminaripentaose + phosphate
laminaritetraose + alpha-D-glucose 1-phosphate
-
-
-
r
laminaripentaose + phosphate
laminaritetraose + alpha-D-glucose 1-phosphate
-
-
-
r
laminaritriose + alpha-D-glucose 1-phosphate
-
-
-
?
laminaritetraose + phosphate
laminaritriose + alpha-D-glucose 1-phosphate
-
-
-
r
laminaritetraose + phosphate
laminaritriose + alpha-D-glucose 1-phosphate
-
-
-
r
laminaribiose + alpha-D-glucose 1-phosphate
-
-
-
?
laminaritriose + phosphate
laminaribiose + alpha-D-glucose 1-phosphate
-
-
-
r
laminaritriose + phosphate
laminaribiose + alpha-D-glucose 1-phosphate
-
-
-
r
?
-
Astasia ocellata
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in presence of arsenate, laminaribiose is completely converted into glucose
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-
?
additional information
?
-
Astasia ocellata
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weak or no activity observed with other carbohydrates
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-
?
additional information
?
-
-
EC 1.4.1.31 and EC 1.4.1.30 catalyse the same reaction but with different quantitative specificity, EC 1.4.1.30 phosphorolyzes laminaritriose and higher homologues at a greater rate than laminaribiose while the opposite behaviour is observed with EC 1.4.1.31
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-
?
additional information
?
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-
arsenolysis of laminaribiose is observed, but not that of glucose 1-phosphate
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-
?
additional information
?
-
-
weak or no activity observed with other carbohydrates
-
-
?
additional information
?
-
-
weak or no activity observed with other carbohydrates
-
-
?
additional information
?
-
on reverse phosphorolysis enzyme is most active on glucose as acceptor and is weakly active on several monosaccharides (e.g. mannose) with alpha-D-glucose 1-phosphate as donor
-
-
?
additional information
?
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no activity with alpha-D-galactosamine 1-phosphate, alpha-D-glucosamine 1-phosphate, alpha-D-galacturonic acid 1-phosphate and alpha-D-galactose 1-phosphate
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-
-