2.4.1.329: sucrose 6F-phosphate phosphorylase

This is an abbreviated version!
For detailed information about sucrose 6F-phosphate phosphorylase, go to the full flat file.

Reaction

Synonyms

RUGNEv3_61221, sucrose 6'-phosphate phosphorylase, YM304_32550

ECTree

     2 Transferases

         2.4 Glycosyltransferases

             2.4.1 Hexosyltransferases

                2.4.1.329 sucrose 6F-phosphate phosphorylase

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Results	in table
269	AA Sequence
3	Cloned(Commentary)
1	Crystallization (Commentary)
12	kcat/KM [mM/s]
16	KM Value [mM]
3	Molecular Weight [Da]
1	Natural Substrates/ Products (Substrates)
6	Organism
2	PDB ID
6	pH Optimum
1	pH Range
14	Protein Variants
1	Reaction
5	Reference
1	Specific Activity [micromol/min/mg]
20	Substrates and Products (Substrate)
5	Subunits
4	Synonyms
1	Systematic Name
2	Temperature Optimum [°C]
3	Temperature Stability [°C]
16	Turnover Number [1/s]

Engineering

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Engineering on EC 2.4.1.329 - sucrose 6F-phosphate phosphorylase

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PROTEIN VARIANTS

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

K364S

Ilumatobacter coccineus

A0A6C7EEG6

mutation to corresponding residue of the Thermoanaerobacterium thermosaccharolyticum enzyme, decrease in kcat value

759364

K434A

Ilumatobacter coccineus

A0A6C7EEG6

strong decrease in kcat value

759364

K434R

Ilumatobacter coccineus

A0A6C7EEG6

mutation to corresponding residue of the Thermoanaerobacterium thermosaccharolyticum enzyme, decrease in kcat value

759364

R152A

Ilumatobacter coccineus

A0A6C7EEG6

mutation to corresponding residue of the Thermoanaerobacterium thermosaccharolyticum enzyme, decrease in kcat value

759364

Y377H

Ilumatobacter coccineus

A0A6C7EEG6

mutation to corresponding residue of the Thermoanaerobacterium thermosaccharolyticum enzyme, decrease in kcat value

759364

K364S

Ilumatobacter coccineus YM16-304

-

mutation to corresponding residue of the Thermoanaerobacterium thermosaccharolyticum enzyme, decrease in kcat value

-

K434A

Ilumatobacter coccineus YM16-304

-

strong decrease in kcat value

-

K434R

Ilumatobacter coccineus YM16-304

-

mutation to corresponding residue of the Thermoanaerobacterium thermosaccharolyticum enzyme, decrease in kcat value

-

R152A

Ilumatobacter coccineus YM16-304

-

mutation to corresponding residue of the Thermoanaerobacterium thermosaccharolyticum enzyme, decrease in kcat value

-

Y377H

Ilumatobacter coccineus YM16-304

-

mutation to corresponding residue of the Thermoanaerobacterium thermosaccharolyticum enzyme, decrease in kcat value

-

H344Y

Thermoanaerobacterium thermosaccharolyticum

D9TT09

decreased ratio of activity on phosphorylated fructose over fructose

726794

K301A

Thermoanaerobacterium thermosaccharolyticum

D9TT09

displays similar activities as the wild type enzyme

726794

R134A

Thermoanaerobacterium thermosaccharolyticum

D9TT09

decreased ratio of activity on phosphorylated fructose over fructose

726794

R135A

Thermoanaerobacterium thermosaccharolyticum

D9TT09

displays similar activities as the wild type enzyme

726794