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2.4.1.337: 1,2-diacylglycerol 3-alpha-glucosyltransferase

This is an abbreviated version!
For detailed information about 1,2-diacylglycerol 3-alpha-glucosyltransferase, go to the full flat file.

Word Map on EC 2.4.1.337

Reaction

UDP-alpha-D-glucose
+
a 1,2-diacyl-sn-glycerol
=
UDP
 +
a 1,2-diacyl-3-O-(alpha-D-glucopyranosyl)-sn-glycerol

Synonyms

alpha-monoglucosyldiacylglycerol synthase, Atu2297, EC 2.4.1.157, MGlcDAG synthase, MGS, UDP-glucose-diacylglycerol glucosyltransferase, UDP-glucose:1,2-diacyl-sn-glycerol 3-D-glucosyltransferase, UDP-glucose:1,2-diacylglycerol 3-D-glucosyltransferase, UDP-glucose:1,2-diacylglycerol glucosyltransferase, UDP-glucose:diacylglycerol glucosyltransferase, UgtP, uridine diphosphoglucose-diacylglycerol glucosyltransferase

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.337 1,2-diacylglycerol 3-alpha-glucosyltransferase

Purification

Purification on EC 2.4.1.337 - 1,2-diacylglycerol 3-alpha-glucosyltransferase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
Binding of the enzyme to lipid bilayers containing biological fractions of anionic lipids is essentially irreversible under most conditions examined. Binding is strongly influenced by anionic lipids, by nonbilayer-prone molecules and by charged polypeptides. Binding to membranes follows a two-step process. The binding is faster and stronger by electrostatic attraction, but hydrophobic interactions are also involved in enhancing the binding and activation process. Once the enzyme is bound to the membrane, it is practically glued in an irreversible fashion
during purification, the concentration of detergent is just as important as the type of detergent, and a low concentration of n-dodecyl-beta-D-maltoside (about 1 x critical micelle concentration) is the best for keeping the protein stable and homogeneous
proteolytic resistance shows a good correlation with the enzyme activity in various lipid-CHAPS mixed micelles. Anionic lipids 1,2-dioleoyl-phosphatidylglycerol and 1,2-dioleoyl-posphatidylserine are able to protect the exposed MGlc-DAG synthase from digestion, whereas 1,2-dioleoyl-phosphatidylcholine and diglucosyldiacylglycerol can not. The detergent dodecylphosphoglycerol can also stimulate the MGlcDAG synthase activity efficiently with a concomitant protection toward proteolytic digestion