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2.4.1.4: amylosucrase

This is an abbreviated version!
For detailed information about amylosucrase, go to the full flat file.

Word Map on EC 2.4.1.4

Reaction

sucrose
+
[(1->4)-alpha-D-glucosyl]n
=
D-fructose
 +
[(1->4)-alpha-D-glucosyl]n+1

Synonyms

AaAS, ACAS, AmAS, AMS, Amy-1, ASASE, BtAS, CcAS, DGAS, DRAS, DRpAS, glucosyltransferase, sucrose-1,4-alpha-glucan, MaAS, MFAS, More, NPAS, NsAS, sucrose-glucan glucosyltransferase, SyAS

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.4 amylosucrase

Purification

Purification on EC 2.4.1.4 - amylosucrase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
Ni-NTA chromatography
nickel affinity chromatography
recombinant C-terminally His6-tagged enzyme by nickel affinity chromatography
-
recombinant enzyme
-
recombinant enzyme from Escherichia coli strain BL21 to homogeneity using GST-affinity chromatography followed by dialysis and subsequent tag removal via PreScission protease and reverse affinity chromatography
-
recombinant GST-tagged ASase from Escherichia coli strain BL21 (DE3) by glutathione affinity chromatography, the tag is cleaved off by thrombin
-
recombinant GST-tagged DgAS by glutathione affinity chromatography, followed by proteolytic removal of the GST-tag
-
recombinant GST-tagged DGAS from Escherichia coli by glutathione affinity chromatography
recombinant GST-tagged DGAS from Escherichia coli strain BL21 by glutathione affinity chromatography
-
recombinant GST-tagged enzyme from Escherichia coli strain JM109 by glutathione affinity chromatography
-
recombinant GST-tagged NpAS by glutathione affinity chromatography, followed by proteolytic removal of the GST-tag
recombinant His-tagged enzyme from Escherichia coli
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and dialysis
-
recombinant His-tagged NPAS from Escherichia coli strain BL21 by nickel affinity chromatography
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3)pLyS by cobalt affinity chromatography
recombinant His6-tagged enzyme from Escherichia coli strain MC1061by nickel affinity chromatography
-
recombinant the His-tagged enzyme from Escherichia coli by nickel affinity chromatography
-
wild-type and mutant enzymes
-