2.4.1.4: amylosucrase
This is an abbreviated version!
For detailed information about amylosucrase, go to the full flat file.
Word Map on EC 2.4.1.4
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2.4.1.4
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neisseria
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polysaccharea
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deinococcus
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geothermalis
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synthesis
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food industry
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biotechnology
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amylose-like
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transglucosylation
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waxy
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drug development
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turanose
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asases
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transglucosidase
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amylopectin
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c-myc-binding
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maltooligosaccharides
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glycoside-hydrolase
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trehalulose
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industry
- 2.4.1.4
- neisseria
- polysaccharea
-
deinococcus
- geothermalis
- synthesis
- food industry
- biotechnology
-
amylose-like
-
transglucosylation
-
waxy
- drug development
- turanose
- asases
- transglucosidase
- amylopectin
-
c-myc-binding
- maltooligosaccharides
-
glycoside-hydrolase
- trehalulose
- industry
Reaction
Synonyms
AaAS, ACAS, AmAS, AMS, Amy-1, ASASE, BtAS, CcAS, DGAS, DRAS, DRpAS, glucosyltransferase, sucrose-1,4-alpha-glucan, MaAS, MFAS, More, NPAS, NsAS, sucrose-glucan glucosyltransferase, SyAS
ECTree
2.4.1.4 amylosucraseAdvanced search results
results (
results (Temperature Stability
Temperature Stability on EC 2.4.1.4 - amylosucrase
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TEMPERATURE STABILITY 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
30
40
40 - 50
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half-life of the enzyme is 421.4 h at 40°C and 3.3 h at 45°C, the thermostability of DRpAS dramatically decreases at 50°C, Tm value is 50.7°C and the half-life of the enzyme is 7.2 min at 50°C
43.2 - 51.5
the half-lives of mutant NPAS-B' and wild-type NPAS are 4.28 and 9.99 min at 45°C and their melting temperatures are 43.25°C and 51.52°C, respectively
45
46
46.1
Tm-value, mutant enzyme R226K/I228V/A289I/F290Y/E300I/V331T/Q437S/N439D/C445A
47.1
Tm-value, mutant enzyme R226K/I228V/A289I/F290Y/E300I/V331T/G396S/T398V/Q437S/N439D/C445R
49
50
50 - 62
half-lives of mutant DGAS-B are 30.70 and 3.39 min at 50°C and 55°C, respectively, whereas that of the wild-type DGAS is 65.74 min at 55°C. The melting temperatures of mutant DGAS-B and wild-type DGAS are 54.23°C and 61.42°C, respectively
50.7
55
60
65
additional information
30
high stability, maintaining 83% activity after 2 h
40
retains more than 90% of its initial activity after incubation for 58 h
40
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the purified recombinant His-tagged ACAS is stable up to 40°C, but rapid loss of activity above, half-life is 1.74 h
45
loses more than 50% of its initial activity after incubation for 1 h
46
Tm-value, mutant enzyme R226K/I228V/A289I/F290Y/E300I/V331T/G396S/T398V/Q437R/N439D
46
Tm-value, mutant enzyme R226L/I228V/F229A/A289I/F290Y/E300I/V331T
50
loses more than 50% of its initial activity after incubation for 5 min
50
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purified recombinant enzyme, half-life is 26 h, denaturation kinetics, overview
50
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half-life of the recombinant wild-type enzyme is 3 min, and of mutant R20C/A451T 32 min, of mutant A170V/Q353L 10 min, and of mutant P351S 24 min
50
complete loss of catalytic activity after 30 min
55
displays more than 90% of its original activity after incubation at 55°C for 5 h
60
retains 73% of its original activity after incubation for 3 h
65
the enzyme keeps half of its original activity after incubation for 1.22 h
additional information
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the quaternary organization is likely to participate in the enhanced thermal stability of the protein
additional information
the quaternary organization is likely to participate in the enhanced thermal stability of the protein
additional information
-
the quaternary organization is likely to participate in the enhanced thermal stability of the protein
