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2.4.1.41: polypeptide N-acetylgalactosaminyltransferase

This is an abbreviated version!
For detailed information about polypeptide N-acetylgalactosaminyltransferase, go to the full flat file.

Word Map on EC 2.4.1.41

Reaction

UDP-N-acetyl-alpha-D-galactosamine
+
[protein]-L-serine
=
UDP
 +
[protein]-3-O-(N-acetyl-alpha-D-galactosaminyl)-L-serine

Synonyms

(UDP)-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase, acetylgalactosaminyltransferase, uridine diphosphoacetylgalactosamine-glycoprotein, bgibmga008904, dGalNAc-T3, Eg-ppGalNAc-T1, GalNAc transferase, GalNAc-T, GalNAc-T1, GalNAc-T11, GalNAc-T12, GalNAc-T13, GalNAc-T14, GalNAc-T16, GalNAc-T18, GalNAc-T2, GalNAc-T3, GalNAc-T4, GalNAc-T5, GalNAc-T6, GalNAc-T7, GalNAc-transferase, GalNAc-transferase T1, GalNAc-transferase T2, GalNAcT4, GALNT1, GALNT11, GALNT12, Galnt13, GALNT14, Galnt18, GALNT2, GALNT3, Galnt4, GalNT5, glycopeptide-preferring polypeptide GalNAc transferase 10, glycoprotein acetylgalactosaminyltransferase, More, N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyl-transferase, N-acetylgalactosaminyltransferase, N-acetylgalactosaminyltransferase-like 1, NM_001079884, PGANT, PGANT1, PGANT2, PGANT3, PGANT4, PGANT5, PGANT6, PGANT7, PGANT8, polypeptide GalNAc transferase, polypeptide GalNAc transferase 2, polypeptide GalNAc-transferase, polypeptide GalNAc-transferase 2, polypeptide GalNAc-transferase-2, polypeptide GalNAcT, polypeptide N-acetylgalactosaminyl transferase-3, polypeptide N-acetylgalactosaminyltransferase, polypeptide N-acetylgalactosaminyltransferase 12, polypeptide N-acetylgalactosaminyltransferase 14, polypeptide N-acetylgalactosaminyltransferase 2, polypeptide N-acetylgalactosaminyltransferase 3, polypeptide N-acetylgalactosaminyltransferase 6, polypeptide N-acetylgalactosaminyltransferase-1, polypeptide-N-acetylgalactosamine transferase, polyppetide GalNAc transferase, pp-GalNAc-T13, pp-GalNAc-T15, pp-GalNAc-T2, pp-GaNTase, ppGalNAc T1, ppGalNAc T10, ppGalNAc T13, ppGalNAc T16, ppGalNAc T2, ppGalNAc T3, ppGalNAc T5, ppGalNAc T6, ppGalNAc-T, ppGalNAc-T1, ppGalNAc-T10, ppGalNAc-T12, ppGalNAc-T2, ppGalNAc-T3, ppGalNAc-T4, ppGalNAc-T5, ppGalNAc-T6, ppGalNAcT, ppGalNAcT or hT, ppGaNTase, ppGaNTase-T1, ppGaNTase-T11, ppGaNTase-T12, ppGaNTase-T3, ppGaNTase-T4, ppGaNTase-T5, ppGaNTase-T6, protein-UDP acetylgalactosaminyltransferase, UDP GalNAc:polypeptide N-acetylgalactosaminyltransferase, UDP N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyl transferase, UDP N-acetyl-alpha-Dgalactosamine:polypeptide N-acetylgalactosaminyl transferase, UDP-acetylgalactosamine-glycoprotein acetylgalactosaminyltransferase, UDP-acetylgalactosamine:peptide-N-galactosaminyltransferase, UDP-GalNAc polypeptide:GalNAc transferase-T2, UDP-GalNAc polypeptide:N-acetylgalactosaminyltransferase, UDP-GalNAc polypeptides:N-acetyl-alpha-galactosaminyltransferase, UDP-GalNAc transferase, UDP-GalNAc:polypeptide alpha-N-acetylgalactosaminyltransferase, UDP-GalNAc:polypeptide alpha-N-acetylgalactosaminyltransferase-2, UDP-GalNAc:polypeptide alpha-N-acetylgalactosaminyltransferase-T1, UDP-GalNAc:polypeptide alphaN-acetylgalactosaminyltransferase, UDP-GalNAc:polypeptide GalNAc transferase, UDP-GalNAc:polypeptide N-acetylgalactosaminyl transferase, UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase, UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 18, UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 3, UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 9, UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase-T3, UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase, UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 1, UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 13, UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 13V1, UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 16, UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 17, UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 18, UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 19, UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 20, UDP-N-acetyl-alpha-D-galactosaminyltransferase, UDP-N-acetyl-D-galactosamine: polypeptide N-acetylgalactosaminyltransferase-6, UDP-N-acetyl-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase-T1, UDP-N-acetyl-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase-T10, UDP-N-acetyl-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase-T3, UDP-N-acetylgalactosamine polypeptide: N-acetylgalactosaminyl-transferase, UDP-N-acetylgalactosamine-glycoprotein N-acetylgalactosaminyltransferase, UDP-N-acetylgalactosamine-protein N-acetylgalactosaminyltransferase, UDP-N-acetylgalactosamine:kappa-casein polypeptide N-acetylgalactosaminyltransferase, UDP-N-acetylgalactosamine:polypeptide N-acetyl-alpha-galactosaminyltransferase, UDP-N-acetylgalactosamine:polypeptide N-acetylgalactosaminyltransferase, UDP-N-acetylgalactosamine:protein N-acetylgalactosaminyl transferase, UDP-N-alpha-D-galactosamine: polypeptide N-acetylgalactosaminyltransferase, UDP-polypeptide N-acetylgalactosaminyl transferase, uridine diphosphate-GalNAc:polypeptide N-acetylgalactosaminyltransferase 3, uridine diphosphoacetylgalactosamine-glycoprotein acetylgalactosaminyltransferase, xGaltnl-1

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.41 polypeptide N-acetylgalactosaminyltransferase

Cloned

Cloned on EC 2.4.1.41 - polypeptide N-acetylgalactosaminyltransferase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
amino acid sequences of ppGaNTase-T3 and –T4
-
cloning and sequenceing of cDNAs encoding several isozymes, DNA sequence analysis
-
cloning of ppGalNAc-T1, -T2, -T3, -T4, -T6 and –T9 and expression as secreted proteins fused with a FLAG peptide in insect cells
-
cloning, sequencing and expression of wild type and SF32 mutant recombinant pgant35A gene in COS7 cells
deletion mutant GalNAc-T1 cDNA is cloned and expressed in COS7 cells
-
enzyme form pp-GalNAc-T15
expressed in COS-7 cells
expressed in Escherichia coli BL21(DE3) cells and HEK-293T cells
expressed in High Five insect cells
-
expressed in Pichia pastoris strain GS115
-
expressed in Sf9 cells or in a stably transfected Chinese hamster ovary cell line exhibited a unique acceptor substrate specificity
expressed in Sf9 cells, expressed in a stably transfected Chinese hamster ovary cell line exhibited a unique acceptor substrate specificity, expressed in High Five cells
expressed in Sf9 insect cells
expressed in Sf9 insect cells Pichia pastoris G115 cells
-
expression in COS-7 cells
-
expression in COS7 cells
Drosophila sp. (in: flies)
-
expression in COS7 cells as soluble His-tagged protein, secretion to the medium
-
expression in COS7 cells as truncated version from pIMKF4 SV40
expression in COS7 cells as truncated version from pInsProA
expression in COS7 cells with an N-terminal hexahistidine tag
-
expression in Escherichia coli
-
expression in HCT-8 cell and HEK-293 cell
expression in HEK-293 cell
expression of GalNAc-T1, -T2 and -T3 as soluble proteins in insect Sf9 cells
-
expression of secreted GalNAc-T4 in CHO cells
-
expression of wild-type and mutants in COS7 cells
-
FLAG fusion protein (amino acid residue 42-607 (catalytic region and lectin domain)) expressed in HEK293T cells
FLAG fusion transmembrane deletion construct expressed in COS7 cells
-
Flag-tagged xGalnt-6 in pCS2+ for eukaryotic expression
-
full-length cDNA is cloned, a soluble enzyme construct prepared by substituting the honeybee melittin leader sequence for the sequences coding for the cytoplasmic and membrane spanning domains is expressed in insect Sf9 cells using a recombinant baculovirus, nucleotide and amino acid sequence
-
full-length GalNAc-T14 in pFlag-CMV2, expression in HEK293T cells
full-length GalNAc-T14 in pGEX-4T-1, expression in Escherichia coli BL21(DE3)
GalNAc-T7 is cloned and expressed in insect Sf9 cells, open reading frame of 1974 bp, GALNT7 gene encoding GalNAc-T7 is localized on chromosome 4q31.1
GalNAc-T9 is cloned and characterized encoding a 603 amino acid protein, nucleotide and amino acid sequence
-
GALNT12, DNA and amino acid sequence determination and analysis, genotyping, recombinant expression of tagged wild-type and mutant enzymes
gene encoding ppGaNTase-T9 from sublingual gland is cloned, characterized, sequenced and expressed transiently in COS7 cells, gene encodes a unique 603-amino acid protein
His tagged truncated protein expressed in High Five cells
-
in pAcGP67-His vector for amplification as bacculovirus in Sf9 cells and subsequent infection of High Five cells
in pKN55-N6His-TEV for expression in Pichia pastoris strain SMD1168 and with TEV-cleavable hexa-His-tag
-
isozymes ppGalNAcT-1, ppGalNAcT-2, and ppGalNAcT-3, recombinant expression of the His-tagged isozymes ppGalNAcT-1, ppGalNAcT-2 in Pichia pastoris and of isozyme ppGalNAcT-3 in COS-7 cells, secretion of the recombinant proteins
-
ORF of xGalntl-1, a C-terminal HA-tagged and a Flag-tagged version in pCS2+ for eukaryotic expression
-
overexpressed in SGC-7901 cell
-
PCR cloning of human version of GalNAc-T1, based on the reported sequence of bovine GalNAc-T1. Polymerase chain reaction cloning and sequencing of the human version of the bovine transferase are presented, and 98% similarity at the amino acid level is found
PCR construct is expressed in insect cells using a baculovirus vector. Expression of GalNAc-T2 in Sf9 cells
PGANT35A in pUAST plasmid for trachea-specific, Gal4-iduceable expression in transgenic flies under control of breathless promoter
pIMKF3 for expression in COS7 cells
-
ppGalNAc-T13 and -T1 are localized to chromosome 2 and 18, ppGalNAc-T13 is cloned and sequenced, 556-amino acid protein
-
ppGalNAc-T13, -T1 and -T9 are cloned, and expressed in Sf21 or High FiveTM insect cells in a soluble form, ppGalNAc-T13: gene is localized to chromosome 2, nucleotide sequence encodes a 556-amino acid protein, ppGalNAc-T1 is localized to chromosome 18
ppGalNAc-T14 and -T2 are cloned and expressed in Sf21 cells in a secreted form, 2 alternatively spliced isoforms of the ppGalNAc-T14 transcript are sequenced, major splicing form: 552-amino acid protein, ppGalNAc-T14 gene is mapped to chromosome 2p23.2
-
ppGaNTase-T1 (mppGaNTase-T1) is expressed as a fusion protein with maltose-binding protein at the N terminus and residues 42–559 of mppGaNTase-T1 at the C terminus separated by a tobacco etch virus protease recognition sequence. The fusion protein is expressed in Pichia pastoris
ppGaNTase-T1 is cloned from kidney RNA, amino acid sequence, expression of ppGaNTases as secreted forms in COS7 cells
-
ppGaNTase-T4 from spleen cDNA library, transient expression in COS7 cellsas secreted soluble and functional enzyme
-
ppGaNTase-T6 from sublingual gland is cloned, sequenced and expressed transiently in COS7 cells as secreted form, 657-amino acid protein, amino acid sequences of ppGaNTase-T1, -T5 and -T6
recombinant human pt-GalNAc-T, which is expressed in insect cells, does not glycosylate several peptides derived from mammalian mucins
soluble His-tagged protein (cytosolic tail and transmembrane domain removed) expressed in Sf9 cells
-
stable functional expression in Drosophila melanogaster S2 cells
stable transfected into Drosophila melanogaster S2 cells
truncated protein expressed in insect cells
-