2.4.1.41: polypeptide N-acetylgalactosaminyltransferase
This is an abbreviated version!
For detailed information about polypeptide N-acetylgalactosaminyltransferase, go to the full flat file.
Word Map on EC 2.4.1.41
-
2.4.1.41
-
o-glycosylation
-
mucin-type
-
mucin
-
glycosyltransferase
-
o-glycans
-
o-linked
-
threonine
-
gangliosides
-
lectin
-
calcinosis
-
oligosaccharide
-
metastasis
-
calcification
-
hyperphosphatemic
-
gm2
-
galactosyltransferase
-
glycolipids
-
1,25-dihydroxyvitamin
-
phosphaturic
-
gd1a
-
sialyltransferase
-
submaxillary
-
angptl3
-
mucin-like
-
klotho
-
hyperostosis
-
muc5ac
-
factor-23
-
apomucin
-
analysis
-
drug development
-
lutropin
-
asn-linked
-
molecular biology
-
periarticular
-
medicine
-
o-glycoproteins
-
iga1
-
villosa
- 2.4.1.41
-
o-glycosylation
-
mucin-type
- mucin
- glycosyltransferase
- o-glycans
-
o-linked
- threonine
- gangliosides
- lectin
- calcinosis
- oligosaccharide
- metastasis
- calcification
-
hyperphosphatemic
- gm2
-
galactosyltransferase
- glycolipids
-
1,25-dihydroxyvitamin
-
phosphaturic
- gd1a
-
sialyltransferase
-
submaxillary
-
angptl3
-
mucin-like
- klotho
- hyperostosis
- muc5ac
-
factor-23
- apomucin
- analysis
- drug development
-
lutropin
-
asn-linked
- molecular biology
-
periarticular
- medicine
-
o-glycoproteins
- iga1
- villosa
Reaction
Synonyms
(UDP)-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase, acetylgalactosaminyltransferase, uridine diphosphoacetylgalactosamine-glycoprotein, bgibmga008904, dGalNAc-T3, Eg-ppGalNAc-T1, GalNAc transferase, GalNAc-T, GalNAc-T1, GalNAc-T11, GalNAc-T12, GalNAc-T13, GalNAc-T14, GalNAc-T16, GalNAc-T18, GalNAc-T2, GalNAc-T3, GalNAc-T4, GalNAc-T5, GalNAc-T6, GalNAc-T7, GalNAc-transferase, GalNAc-transferase T1, GalNAc-transferase T2, GalNAcT4, GALNT1, GALNT11, GALNT12, Galnt13, GALNT14, Galnt18, GALNT2, GALNT3, Galnt4, GalNT5, glycopeptide-preferring polypeptide GalNAc transferase 10, glycoprotein acetylgalactosaminyltransferase, More, N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyl-transferase, N-acetylgalactosaminyltransferase, N-acetylgalactosaminyltransferase-like 1, NM_001079884, PGANT, PGANT1, PGANT2, PGANT3, PGANT4, PGANT5, PGANT6, PGANT7, PGANT8, polypeptide GalNAc transferase, polypeptide GalNAc transferase 2, polypeptide GalNAc-transferase, polypeptide GalNAc-transferase 2, polypeptide GalNAc-transferase-2, polypeptide GalNAcT, polypeptide N-acetylgalactosaminyl transferase-3, polypeptide N-acetylgalactosaminyltransferase, polypeptide N-acetylgalactosaminyltransferase 12, polypeptide N-acetylgalactosaminyltransferase 14, polypeptide N-acetylgalactosaminyltransferase 2, polypeptide N-acetylgalactosaminyltransferase 3, polypeptide N-acetylgalactosaminyltransferase 6, polypeptide N-acetylgalactosaminyltransferase-1, polypeptide-N-acetylgalactosamine transferase, polyppetide GalNAc transferase, pp-GalNAc-T13, pp-GalNAc-T15, pp-GalNAc-T2, pp-GaNTase, ppGalNAc T1, ppGalNAc T10, ppGalNAc T13, ppGalNAc T16, ppGalNAc T2, ppGalNAc T3, ppGalNAc T5, ppGalNAc T6, ppGalNAc-T, ppGalNAc-T1, ppGalNAc-T10, ppGalNAc-T12, ppGalNAc-T2, ppGalNAc-T3, ppGalNAc-T4, ppGalNAc-T5, ppGalNAc-T6, ppGalNAcT, ppGalNAcT or hT, ppGaNTase, ppGaNTase-T1, ppGaNTase-T11, ppGaNTase-T12, ppGaNTase-T3, ppGaNTase-T4, ppGaNTase-T5, ppGaNTase-T6, protein-UDP acetylgalactosaminyltransferase, UDP GalNAc:polypeptide N-acetylgalactosaminyltransferase, UDP N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyl transferase, UDP N-acetyl-alpha-Dgalactosamine:polypeptide N-acetylgalactosaminyl transferase, UDP-acetylgalactosamine-glycoprotein acetylgalactosaminyltransferase, UDP-acetylgalactosamine:peptide-N-galactosaminyltransferase, UDP-GalNAc polypeptide:GalNAc transferase-T2, UDP-GalNAc polypeptide:N-acetylgalactosaminyltransferase, UDP-GalNAc polypeptides:N-acetyl-alpha-galactosaminyltransferase, UDP-GalNAc transferase, UDP-GalNAc:polypeptide alpha-N-acetylgalactosaminyltransferase, UDP-GalNAc:polypeptide alpha-N-acetylgalactosaminyltransferase-2, UDP-GalNAc:polypeptide alpha-N-acetylgalactosaminyltransferase-T1, UDP-GalNAc:polypeptide alphaN-acetylgalactosaminyltransferase, UDP-GalNAc:polypeptide GalNAc transferase, UDP-GalNAc:polypeptide N-acetylgalactosaminyl transferase, UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase, UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 18, UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 3, UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 9, UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase-T3, UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase, UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 1, UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 13, UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 13V1, UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 16, UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 17, UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 18, UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 19, UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 20, UDP-N-acetyl-alpha-D-galactosaminyltransferase, UDP-N-acetyl-D-galactosamine: polypeptide N-acetylgalactosaminyltransferase-6, UDP-N-acetyl-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase-T1, UDP-N-acetyl-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase-T10, UDP-N-acetyl-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase-T3, UDP-N-acetylgalactosamine polypeptide: N-acetylgalactosaminyl-transferase, UDP-N-acetylgalactosamine-glycoprotein N-acetylgalactosaminyltransferase, UDP-N-acetylgalactosamine-protein N-acetylgalactosaminyltransferase, UDP-N-acetylgalactosamine:kappa-casein polypeptide N-acetylgalactosaminyltransferase, UDP-N-acetylgalactosamine:polypeptide N-acetyl-alpha-galactosaminyltransferase, UDP-N-acetylgalactosamine:polypeptide N-acetylgalactosaminyltransferase, UDP-N-acetylgalactosamine:protein N-acetylgalactosaminyl transferase, UDP-N-alpha-D-galactosamine: polypeptide N-acetylgalactosaminyltransferase, UDP-polypeptide N-acetylgalactosaminyl transferase, uridine diphosphate-GalNAc:polypeptide N-acetylgalactosaminyltransferase 3, uridine diphosphoacetylgalactosamine-glycoprotein acetylgalactosaminyltransferase, xGaltnl-1
ECTree
2.4.1.41 polypeptide N-acetylgalactosaminyltransferaseAdvanced search results
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results (Substrates Products
Substrates Products on EC 2.4.1.41 - polypeptide N-acetylgalactosaminyltransferase
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SUBSTRATE 
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
2 UDP-N-acetyl-alpha-D-galactosamine + polypeptide Muc1a
2 UDP + N-acetyl-alpha-D-galactosaminyl-polypeptide Muc1a
-
sequence APPAHGVTSAPDTRPAPGC
-
-
?
2 UDP-N-acetyl-alpha-D-galactosamine + polypeptide Muc1a'
2 UDP + N-acetyl-alpha-D-galactosaminyl-polypeptide Muc1a'
-
sequence AHGVTSAPDTR
-
-
?
5 UDP-N-acetyl-alpha-D-galactosamine + beta1-adrenergic receptor
5 UDP + (N-acetyl-alpha-D-galactosaminyl)5-beta1-adrenergic receptor
-
-
-
?
5 UDP-N-acetyl-D-galactosamine + DSTTPAPTTK
5 UDP + N-acetyl-D-galactosaminylated DSTTPAPTTK
IgA hinge-4GalNAc + UDP-GalNAc
IgA hinge-nGalNAc + UDP
-
-
-
?
MUC1-2GalNAc + UDP-GalNAc
MUC1-nGalNAc + UDP
-
-
-
?
MUC2-6GalNAc + UDP-GalNAc
MUC2-nGalNAc + UDP
-
-
-
?
MUC5AC + UDP-GalNAc
GalNAc-Thr9-MUC5AC + UDP
-
hT2CD, hT2: glycosylation site selection driven by and substrate binding through its catalytic domain, hT10: not catalysed by hT10 because hT10 does not recognize naked peptides
preferred site for glycosylation by hT2 and hT2CD compared to Thr-3, Thr-10 and Thr-13
-
?
MUC5AC glycopeptides + UDP-GalNAc
GalNAc-MUC5AC + UDP
-
hT2: site preference of glycosylation depends on location of pre-existing GalNAc and is mediated by its lectin domain which directs glycosylation 10 residues N- or C-terminal to an extant GalNAc residue and aids site selection only when potential site is N-terminal to an extant GalNAc residue not C-terminal, Thr-9 is no preferred site of glycosylation, hT10: must recognize existing GalNAc residues via its catalytic domain which directs selection to the glycosylation site immediately N-terminal to the present GalNAc,
-
-
?
MUC5AC-13 + UDP-GalNAc
GalNAc-Thr12-MUC5AC-13 + UDP
-
hT10, hT10CD, hT10CD-hT2LD
-
-
?
MUC5AC-13 + UDP-GalNAc
GalNAc-Thr3-MUC5AC-13 + UDP
-
hT2: substrate binding through its catalytic domain, hT10: transfer of only single GalNAc residues
predominant product of hT2 and hT10
-
?
MUC5AC-13 + UDP-GalNAc
GalNAc-Thr9-MUC5AC-13 + UDP
-
hT2, hT2CD: no initial burst phase during catalysis
not preferred by hT2, preferred by hT2CD
-
?
MUC5AC-3 + UDP-GalNAc
GalNAc-Thr13-MUC5AC-3 + UDP
-
hT2: no initial burst phase during catalysis, substrate binding through its catalytic domain, hT10: transfer of only single GalNAc residues
hT2: preferred site for glycosylation, mediated by enhanced product release through lectin domain, hT10: predominant product
-
?
MUC5AC-3 + UDP-GalNAc
GalNAc-Thr2-MUC5AC + UDP
-
hT10CD, hT10CD-hT2LD, hT10: catalysis faster than for MUCAC-9
-
-
?
MUC5AC-3 + UDP-GalNAc
GalNAc-Thr9-MUC5AC-3 + UDP
-
hT2, hT2CD
not preferred by hT2, hT2CD: preferred site for glycosylation, lack of lectin domain in hT2CD results in rate-limiting product release
-
?
MUC5AC-3,13 + UDP-GalNAc
GalNAc-Ser-5-MUC5AC-3,13 + UDP
-
hT2, not catalysed by hT2CD and hT2CD-hT10LD
preferred site by hT2
-
?
MUC5AC-3,13 + UDP-GalNAc
GalNAc-Thr2,12-MUC5AC-3,13 + UDP
-
hT10, hT10CD, hT10CD-hT2LD
-
-
?
MUC5AC-9 + UDP-GalNAc
GalNAc-Thr2-MUC5AC-9 + UDP
-
not catalysed by hT10CD and hT10CD-hT2LD, hT10: catalysis slower than for MUC5AC-3
preferred site of glycosylation by hT10
-
?
OSM fragment + UDP-Gal
OSM fragment-nGalNAc + UDP
-
-
-
?
UDP-alpha-GalNAc + polypeptide
GalNAc-alpha-polypeptide + UDP
-
major mucin-type O-glycan in embryonic tracheal system along the apical region and luman of the tracheal tubes O-glycan, detectable by lectins Jacalin, soybean agglutinin and Vicia villosa agglutinin
-
?
UDP-GalNAc + apomucin
UDP + GalNAc-mucin
apomucin is a deglycosylated bovine submaxillary mucin, mutants W328Y, W328F, W328A, W316H and W316A are inactive, mutants W316Y and W316F show 40% and 20% activity, respectively, relative to wild-type
-
-
?
UDP-GalNAc + AQPTPPP
UDP + AQP-(GalNAcalpha1-)TPPP
-
pH 7.5, 37°C, 0.2 mM, 100% activity
-
-
?
UDP-GalNAc + GAGA(X)3T(X)3AGAGK
GAGA(X)3(GalNAc)T(X)3AGAGK + UDP
-
-
-
?
UDP-GalNAc + GVVPTVVPG
UDP + GVVP(GalNAc)TVVPG
GVVPTVVPG is a synthetic peptide with threonine as acceptor site, weaker acceptor substrate for wild-type and truncated version P-T1/delta42 as PPDAATAAPL
-
-
?
UDP-GalNAc + N-acetyl-4-benzoyl-L-Phe-L-Pro-L-Tyr-L-Pro-L-Pro-L-Pro-NH2
UDP + N-acetyl-4-benzoyl-L-Phe-L-Pro-L-(GalNAcalpha1-)Tyr-L-Pro-L-Pro-L-Pro-NH2
-
pH 7.5, 37°C
-
-
?
UDP-GalNAc + polypeptide
GalNAc-alpha-polypeptide + UDP
-
-
glycosylation at Thr or Ser
-
?
UDP-GalNAc + PPDAATAAPL
UDP + PPDAA(GalNAc)TAAPL
PPDAATAAPL is a synthetic peptide with threonine as acceptor site, efficient acceptor substrate for wild-type and truncated version P-T1/delta42
-
-
?
UDP-GalNAc + PRFODSSSKAPPPLPSPSRLPG
GalNAc-PRFODSSSKAPPPLPSPSRLPG + UDP
-
-
-
-
?
UDP-GalNAc + PTTTPITTTG
UDP + P-(GalNAcalpha1-)TTTPITTTG + P-(GalNAcalpha1-)TTTPI-(GalNAcalpha1-)TTTG
-
pH 7.5, 37°C, 0.2 mM, 26% activity relative to AQPTPPP
-
-
?
UDP-GalNAc + selectin
GalNAc-selectin + UDP
-
-
reduced expression of cell surface E- and P-selectins (fourfold and fivefold) and L-selectins on immune cells of ppGalNAcT-1 deficient mice
-
?
UDP-GalNAc + TPTGTQTPTG
UDP + TPTG-(GalNAcalpha1-)TQTPTG
-
pH 7.5, 37°C, 0.2 mM, 8% activity relative to AQPTPPP
-
-
?
UDP-GalNAc + TTTVTPTPTG
UDP + TTTV-(GalNAcalpha1-)TPTPTG + T-(GalNAcalpha1-)TTV-(GalNAcalpha1-)TPTPTG + T-(GalNAcalpha1-)T-(GalNAcalpha1-)TV-(GalNAcalpha1-)TP-(GalNAcalpha1-)TPTG + T-(GalNAcalpha1-)TTV-(GalNAcalpha1-)TP-(GalNAcalpha1-)TP-(GalNAcalpha1-)TG + TT-(GalNAcalpha1-)TV-(GalNAcalpha1-)TPTPTG + ?
-
pH 7.5, 37°C, 0.2 mM, 109% activity relative to AQPTPPP
monoglycosylated product appears after 30 min, higher glycosylated products appear with increasing time of incubation
-
?
UDP-GalNAc + TVTPTPTPTG
UDP + TVTP-(GalNAcalpha1-)TPTPTG
-
pH 7.5, 37°C, 0.2 mM, 145% activity relative to AQPTPPP
-
-
?
UDP-N-((2S)-2-azidopropanoyl)-alpha-D-galactosamine + peptide EA2-biotin
UDP + N-((2S)-2-azidopropanoyl)-alpha-D-galactosaminyl-peptide EA2-biotin
i.e. PTTDSTTPAPTTKK-biotin
-
-
-
UDP-N-acetyl-alpha-D-galactosamine + (glycosylated GTTPSPVPTTSTTSAP)
UDP + ?
-
natural-type (alpha-GalNAc-O-Thr) and unnatural-type (beta-GalNAc-O-Thr, alpha-Fuc-O-Thr and beta-GlcNAc-O-Thr) glycosylated
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + Ac-CIRIQRGPGRAFVTIGKIGNMR
UDP + ?
-
-
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + Ac-Pro-Thr-Thr-Thr-Pro-Ile-Ser-Thr-NH2
?
-
-
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + deglycosylated human trachea mucin
?
-
-
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + deglycosylated swine trachea mucin
?
-
-
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + delta-opioid receptor
UDP + N-acetyl-alpha-D-galactosaminyl-delta-opioid receptor
-
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + EA2 peptide
UDP + N-acetyl-alpha-D-galactosaminyl-EA2 peptide
EA peptide, i.e. PTTDSTTPAPTTK
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + Gln-Ala-Ala-Gly-Thr-Ser-Gly-Ala-Gly-Pro-Gly
?
-
-
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + Gly-Val-Val-Pro-Thr-Val-Val-Pro-Gly
?
-
-
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + gp40-derived peptide
UDP + N-acetyl-alpha-D-galactosaminyl-gp40-derived peptide
-
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + GTTPSPVPTTSTTSAP
UDP + GT[GalNAc]TPSPVPTTSTTSAP
-
-
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + GTTPSPVPTTSTTSAP
UDP + GT[GalNAc]TPSPVPTTST[GalNAc]TSAP
-
-
glycosylation at Thr-3 and Thr-13
-
?
UDP-N-acetyl-alpha-D-galactosamine + GTTPSPVPTTSTTSAP
UDP + GT[GalNAc]TP[GalNAc]SPVPTTSTTSAP
-
-
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + GTTPSPVPTTST[GalNAc]TSAP
UDP + GT[GalNAc]TPSPVPTTST[GalNAc]TSAP
-
-
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + GTTPSPVPTTST[GalNAc]TSAP
UDP + GT[GalNAc]TP[GalNAc]SPVPTTST[GalNAc]TSAP
-
-
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + GT[GalNAc]TPSPVPTTSTTSAP
UDP + GT[GalNAc]TPSPVPTTS[GalNAc]T[GalNAc]TSAP
-
-
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + GT[GalNAc]TPSPVPTTSTTSAP
UDP + G[GalNAc]T[GalNAc]TPSPVPTTST[GalNAc]TSAP
-
-
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + GT[GalNAc]TPSPVPTTST[GalNAc]TSAP
UDP + GT[GalNAc]TPSPVPTTS[GalNAc]T[GalNAc]TSAP
-
-
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + human proteoglycan 4
UDP + N-acetyl-alpha-D-galactosaminyl-(human proteoglycan 4)
-
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + mucin-type O-glycoprotein podoplanin
UDP + N-acetyl-alpha-D-galactosaminyl-mucin-type O-glycoprotein podoplanin
-
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + neuroglycan
UDP + N-acetyl-alpha-D-galactosaminyl-neuroglycan
-
-
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + peptide EA2
UDP + N-acetyl-alpha-D-galactosaminyl-(peptide EA2)
-
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + peptide EA2-biotin
UDP + N-acetyl-alpha-D-galactosaminyl-peptide EA2-biotin
i.e. PTTDSTTPAPTTKK-biotin
-
-
-
UDP-N-acetyl-alpha-D-galactosamine + polypeptide
UDP + N-acetyl-alpha-D-galactosaminyl-polypeptide
UDP-N-acetyl-alpha-D-galactosamine + polypeptide ActR-IIB
UDP + N-acetyl-alpha-D-galactosaminyl-polypeptide ActR-IIB
-
-
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + polypeptide angiopoietin-like 3
UDP + N-acetyl-alpha-D-galactosaminyl-polypeptide angiopoietin-like 3
-
-
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + polypeptide biotin-EA2
UDP + N-acetyl-alpha-D-galactosaminyl-polypeptide biotin-EA2
UDP-N-acetyl-alpha-D-galactosamine + polypeptide biotin-EA2-2
UDP + N-acetyl-alpha-D-galactosaminyl-polypeptide biotin-EA2-2
UDP-N-acetyl-alpha-D-galactosamine + polypeptide CHT 1
UDP + N-acetyl-alpha-D-galactosaminyl-polypeptide CHT 1
-
sequence APPAHPGPTPGPRPAPG
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + polypeptide CHT 10
UDP + N-acetyl-alpha-D-galactosaminyl-polypeptide CHT 10
-
sequence APPAHPGPTIGPRPAPG
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + polypeptide CHT 11
UDP + N-acetyl-alpha-D-galactosaminyl-polypeptide CHT 11
-
sequence APPAHPGPTPRPRPAPG
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + polypeptide CHT 12
UDP + N-acetyl-alpha-D-galactosaminyl-polypeptide CHT 12
-
sequence APPAHHGPTPGPRPAPG
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + polypeptide CHT 13
UDP + N-acetyl-alpha-D-galactosaminyl-polypeptide CHT 13
-
sequence APPAHPVPTPGPRPAPG
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + polypeptide CHT 14
UDP + N-acetyl-alpha-D-galactosaminyl-polypeptide CHT 14
-
sequence APPAHPGLTPGPRPAPG
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + polypeptide CHT 15
UDP + N-acetyl-alpha-D-galactosaminyl-polypeptide CHT 15
-
sequence APPAHHVVTERYRPAPG
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + polypeptide CHT 16
UDP + N-acetyl-alpha-D-galactosaminyl-polypeptide CHT 16
-
sequence APPAHDFVTPAPRPAPG
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + polypeptide CHT 17
UDP + N-acetyl-alpha-D-galactosaminyl-polypeptide CHT 17
-
sequence APPAHDFPTPAPRPAPG
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + polypeptide CHT 18
UDP + N-acetyl-alpha-D-galactosaminyl-polypeptide CHT 18
-
sequence APPAHPFVTPAPRPAPG
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + polypeptide CHT 19
UDP + N-acetyl-alpha-D-galactosaminyl-polypeptide CHT 19
-
sequence APPAHIFVTPAPRPAPG
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + polypeptide CHT 2
UDP + N-acetyl-alpha-D-galactosaminyl-polypeptide CHT 2
-
sequence APPAHPGVTPGPRPAPG
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + polypeptide CHT 20
UDP + N-acetyl-alpha-D-galactosaminyl-polypeptide CHT 20
-
sequence APPAHVFVTPAPRPAPG
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + polypeptide CHT 21
UDP + N-acetyl-alpha-D-galactosaminyl-polypeptide CHT 21
-
sequence APPAHDGVTPAPRPAPG
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + polypeptide CHT 22
UDP + N-acetyl-alpha-D-galactosaminyl-polypeptide CHT 22
-
sequence APPAHPGPTPAPRPAPG
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + polypeptide CHT 23
UDP + N-acetyl-alpha-D-galactosaminyl-polypeptide CHT 23
-
sequence PSSSPISSSSSVSPSPSPSGSQSK
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + polypeptide CHT 3
UDP + N-acetyl-alpha-D-galactosaminyl-polypeptide CHT 3
-
sequence APPAHPGPTPGYRPAPG
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + polypeptide CHT 4
UDP + N-acetyl-alpha-D-galactosaminyl-polypeptide CHT 4
-
sequence APPAHPGPTPGHRPAPG
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + polypeptide CHT 5
UDP + N-acetyl-alpha-D-galactosaminyl-polypeptide CHT 5
-
sequence APPAHPGPTPGHRPAPG
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + polypeptide CHT 6
UDP + N-acetyl-alpha-D-galactosaminyl-polypeptide CHT 6
-
sequence APPAHPGVTPGYRPAPG
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + polypeptide CHT 7
UDP + N-acetyl-alpha-D-galactosaminyl-polypeptide CHT 7
-
sequence APPAHPGITPGPRPAPG
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + polypeptide CHT 8
UDP + N-acetyl-alpha-D-galactosaminyl-polypeptide CHT 8
-
sequence APPAHPGFTPGPRPAPG
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + polypeptide CHT 9
UDP + N-acetyl-alpha-D-galactosaminyl-polypeptide CHT 9
-
sequence APPAHPGPTEGPRPAPG
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + polypeptide EA2
UDP + N-acetyl-alpha-D-galactosaminyl-polypeptide EA2
UDP-N-acetyl-alpha-D-galactosamine + polypeptide EA2-biotin
UDP + N-acetyl-alpha-D-galactosaminyl-polypeptide EA2-biotin
UDP-N-acetyl-alpha-D-galactosamine + polypeptide FGF23
UDP + N-acetyl-alpha-D-galactosaminyl-polypeptide FGF23
-
-
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + polypeptide GPIV
UDP + N-acetyl-alpha-D-galactosaminyl-polypeptide GPIV
UDP-N-acetyl-alpha-D-galactosamine + polypeptide GPIV-C
UDP + N-acetyl-alpha-D-galactosaminyl-polypeptide GPIV-C
-
-
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + polypeptide GPV
UDP + N-acetyl-alpha-D-galactosaminyl-polypeptide GPV
UDP-N-acetyl-alpha-D-galactosamine + polypeptide GPV-C
UDP + N-acetyl-alpha-D-galactosaminyl-polypeptide GPV-C
UDP-N-acetyl-alpha-D-galactosamine + polypeptide GRA2-biotin
UDP + N-acetyl-alpha-D-galactosaminyl-polypeptide GRA2-biotin
UDP-N-acetyl-alpha-D-galactosamine + polypeptide Muc1
UDP + N-acetyl-alpha-D-galactosaminyl-polypeptide Muc1
-
-
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + polypeptide Muc10
UDP + N-acetyl-alpha-D-galactosaminyl-polypeptide Muc10
-
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + polypeptide MUC13
UDP + N-acetyl-alpha-D-galactosaminyl-polypeptide MUC13
-
isoform GALNT14 contributes to the glycosylation of peptide MUC13, which is significantly higher in ovarian cancer cells compared with the normal/benign ovary tissues
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + polypeptide Muc1fr1
UDP + N-acetyl-alpha-D-galactosaminyl-polypeptide Muc1fr1
-
-
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + polypeptide Muc1fr2
UDP + N-acetyl-alpha-D-galactosaminyl-polypeptide Muc1fr2
-
-
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + polypeptide Muc1fr3
UDP + N-acetyl-alpha-D-galactosaminyl-polypeptide Muc1fr3
-
-
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + polypeptide Muc2
UDP + N-acetyl-alpha-D-galactosaminyl-polypeptide Muc2
UDP-N-acetyl-alpha-D-galactosamine + polypeptide Muc5Ac
UDP + N-acetyl-alpha-D-galactosaminyl-polypeptide Muc5Ac
UDP-N-acetyl-alpha-D-galactosamine + polypeptide MUC5AC-3,13-biotin
UDP + N-acetyl-alpha-D-galactosaminyl-polypeptide MUC5AC-3,13-biotin
UDP-N-acetyl-alpha-D-galactosamine + polypeptide OPSS1
UDP + N-acetyl-alpha-D-galactosaminyl-polypeptide OPSS1
-
-
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + polypeptide OPSS4
UDP + N-acetyl-alpha-D-galactosaminyl-polypeptide OPSS4
-
-
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + polypeptide RNApII
UDP + N-acetyl-alpha-D-galactosaminyl-polypeptide RNApII
-
-
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + Pro-Pro-Ala-Ser-Thr-Ser-Ala-Pro-Gly
?
-
-
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + Pro-Pro-Asp-Ala-Ala-Thr-Ala-Ala-Pro-Leu
?
-
-
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + Pro-Pro-Asp-Ala-Ser-Ser-Ser-Ala-Pro-Leu-Arg
?
-
-
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + Pro-Pro-Asp-Val-Val-Ser-Val-Val-Pro-Leu-Arg
?
-
-
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + Val-Leu-Gly-Thr-Thr-Ala-Val
?
-
-
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + Val-Lys-Thr-Glu-Ala-Thr-Thr-Phe-Ile
?
-
-
-
-
?
UDP-N-acetyl-D-galactosamine + A1 protein
UDP + N-acetyl-D-galactosaminyl-A1 protein
-
-
-
-
?
UDP-N-acetyl-D-galactosamine + Ac-CIRIQRGPGRAFVTIGKIGNMR
UDP + N-acetyl-D-galactosaminylated Ac-CIRIQRGPGRAFVTIGKIGNMR
-
-
-
?
UDP-N-acetyl-D-galactosamine + Ac-PFVTHPGYD
UDP + N-acetyl-D-galactosaminylated Ac-PFVTHPGYD
-
-
-
?
UDP-N-acetyl-D-galactosamine + Ac-QATEYEYLDYDFLPETEPPEM
UDP + N-acetyl-D-galactosaminylated Ac-QATEYEYLDYDFLPETEPPEM
UDP-N-acetyl-D-galactosamine + AcTPPP
UDP + N-acetyl-D-galactosaminyl-AcTPPP
-
-
-
-
?
UDP-N-acetyl-D-galactosamine + AHGVTSAPDTR
UDP + N-acetyl-D-galactosaminylated AHGVTSAPDTR
-
-
-
?
UDP-N-acetyl-D-galactosamine + apoantifreeze glycoproteins
UDP + N-acetyl-D-galactosaminyl-apoantifreeze glycoproteins
-
apoantifreeze glycoprotein of high molecular weight accepts GalNAc much faster than apoantifreeze glycoprotein 8
-
-
?
UDP-N-acetyl-D-galactosamine + apofetuin
UDP + N-acetyl-D-galactosaminyl-apofetuin
-
lowest rate of transfer
-
-
?
UDP-N-acetyl-D-galactosamine + bone sialoprotein
UDP + UDP-N-acetyl-D-galactosaminyl-bone sialoprotein
-
preferred substrate of isozyme ppGalNAcT-1, glycosylation of Thr101, Ser131, Thr199, and Ser214, glycosylation pattern, overview
-
-
?
UDP-N-acetyl-D-galactosamine + CPPTPSATTPAPPSSSAPPETTAA
UDP + N-acetyl-D-galactosaminylated CPPTPSATTPAPPSSSAPPETTAA
UDP-N-acetyl-D-galactosamine + deglycosylated ovine submaxillary mucin
UDP + ?
-
-
-
-
?
UDP-N-acetyl-D-galactosamine + fibronectin
UDP + N-acetyl-D-galactosaminyl-fibronectin
-
fibronectin is a physiological substrate for GalNAc-T3
-
-
?
UDP-N-acetyl-D-galactosamine + GAGAPGPTPGPAGAGK
UDP + GAGAPGP-(N-acetyl-D-galactosaminylT)-PGPAGAGK
-
optimal isozyme ppGalNAc T2 peptide substrate
-
-
?
UDP-N-acetyl-D-galactosamine + GalNAc-glycosylated peptide
UDP + N-acetyl-D-galactosaminyl-GalNAc-glucosylated peptide
UDP-N-acetyl-D-galactosamine + GKPRPYSPRPTSHPRPIRV
UDP + ?
i.e. drosocin, low activity, enzyme form PGANT8
-
-
?
UDP-N-acetyl-D-galactosamine + GTTPSPVPTTSTTSA
UDP + N-acetyl-D-galactosaminyl-[GTTPSPVPTTSTTSA]
i.e. Muc5Ac peptide, multiple possible binding sites for N-acetyl-D-galactosamine on the peptide
-
-
?
UDP-N-acetyl-D-galactosamine + HIV-V3 peptide
UDP + N-acetyl-D-galactosaminyl-HIV-V3 peptide
UDP-N-acetyl-D-galactosamine + human chorionic gonadotropin-beta peptide
UDP + N-acetyl-D-galactosaminyl-human chorionic gonadotropin-beta peptide
UDP-N-acetyl-D-galactosamine + immunoglobulin A1
UDP + N-acetyl-D-galactosaminyl-immunoglobulin A1
-
O-linked glycosylation of the human IgA1 hinge region by ppGalNAc-T2, synthetic IgA hinge peptide: ppGalNAc-T2 shows strongest activity and is able to transfer GalNAc to almost all possible glycosylation sites, other ppGalNAc-Ts show extremely weak activities
-
-
?
UDP-N-acetyl-D-galactosamine + mucin MUC4AC-13
UDP + ?
enzyme form PGANT2
-
-
?
UDP-N-acetyl-D-galactosamine + myelin basic protein
UDP + N-acetyl-D-galactosaminyl-myelin basic protein
UDP-N-acetyl-D-galactosamine + PHMAQVTVGPGL
UDP + N-acetyl-D-galactosaminyl-PHMAQVTVGPGL
-
-
-
-
?
UDP-N-acetyl-D-galactosamine + PPDAATAAPLR
UDP + N-acetyl-D-galactosaminyl-PPDAATAAPLR
-
i.e. EPO-T peptide
-
-
?
UDP-N-acetyl-D-galactosamine + PRFQDSSSSKAPPPSLPSPSRL
UDP + N-actetyl-D-galactosaminylated PRFQDSSSSKAPPPSLPSPSRL
-
-
-
?
UDP-N-acetyl-D-galactosamine + PRFQDSSSSKAPPPSLPSPSRLPG
UDP + N-acetyl-D-galactosaminylated PRFQDSSSSKAPPPSLPSPSRLPG
-
-
-
?
UDP-N-acetyl-D-galactosamine + PTTTPISTTMVTPTPTPTC
UDP + N-actetyl-D-galactosaminylated PTTTPISTTMVTPTPTPTC
-
-
-
?
UDP-N-acetyl-D-galactosamine + PTTTPISTTTMVTPTPTPTC
UDP + N-acetyl-D-galactosaminylated PTTTPISTTTMVTPTPTPTC
mucin derived substrate
-
-
?
UDP-N-acetyl-D-galactosamine + QATEYEYLDYDFLPEC
UDP + N-acetyl-D-galactosaminylated QATEYEYLDYDFLPEC
-
-
-
?
UDP-N-acetyl-D-galactosamine + rhodanese
UDP + N-acetyl-D-galactosaminyl-rhodanese
-
reduced and carboxylmethylated bovine rhodanese, at a lower rate than with myelin basic protein
-
-
?
UDP-N-acetyl-D-galactosamine + RPAPGSTAPPA
UDP + N-acetyl-D-galactosaminylated RPAPGSTAPPA
-
-
-
?
UDP-N-acetyl-D-galactosamine + SATTPAPPSSG
UDP + ?
Drosophila sp. (in: flies)
-
preferred substrate
-
-
?
UDP-N-acetyl-D-galactosamine + subtilisin
UDP + N-acetyl-D-galactosaminyl-subtilisin
-
reduced and carboxylmethylated bacterial subtilisin, at a lower rate than with myelin basic protein or bovine rhodanese
-
-
?
UDP-N-acetyl-D-galactosamine + syndecan-3
UDP + N-acetyl-D-galactosaminyl-syndecan-3
syndecan-3 may be a natural substrate for GalNAc-T13
-
-
?
UDP-N-acetyl-D-galactosamine + TAPPAHGVTSAPDTRPAPGSTAPP
UDP + N-acetyl-D-galactosaminylated TAPPAHGVTSAPDTRPAPGSTAPP
UDP-N-hex-5-ynoyl-alpha-D-galactosamine + peptide EA2-biotin
UDP + N-hex-5-ynoyl-alpha-D-galactosaminyl-peptide EA2-biotin
i.e. PTTDSTTPAPTTKK-biotin
-
-
-
5 UDP-N-acetyl-D-galactosamine + DSTTPAPTTK
5 UDP + N-acetyl-D-galactosaminylated DSTTPAPTTK
mucin derived substrate
-
-
?
5 UDP-N-acetyl-D-galactosamine + DSTTPAPTTK
5 UDP + N-acetyl-D-galactosaminylated DSTTPAPTTK
mucin derived substrate, acceptor substrate
-
-
?
erythropoietin T + UDP-Gal
erythropoietin T-nGalNAc + UDP
-
-
-
?
erythropoietin T + UDP-Gal
erythropoietin T-nGalNAc + UDP
also catalysed by mutants GalTNAc-T2-D458H and GalTNAc-T2-D541A
-
-
?
IgA hinge + UDP-GalNAc
IgA hinge-nGalNAc + UDP
-
-
-
?
IgA hinge + UDP-GalNAc
IgA hinge-nGalNAc + UDP
GalTNAc-T11 incorporates only two GalNAc residues into IgA hinge peptide
-
-
?
IgA hinge + UDP-GalNAc
IgA hinge-nGalNAc + UDP
mutant GalNAc-T2-D458H incorporates 1-2 fewer GalNAc residues than wild-type
-
-
?
MUC1 + UDP-GalNAc
MUC1-nGalNAc + UDP
GalNAc-T4 lectin domain does not exhibit selective specificities for peptide sequences, or the density or pattern of GalNAc glycosylation, GalNAc-T4 lectin domain mediates GalNAc-glycopeptide substrate specificity
-
-
?
MUC1 + UDP-GalNAc
MUC1-nGalNAc + UDP
mutant GalNAc-T2-D458H incorporates 1-2 fewer GalNAc residues than wild-type, GalNAc-T2 lectin domain does not exhibit selective specificities for peptide sequences, or the density or pattern of GalNAc glycosylation
-
-
?
UDP-galactose + Muc1 peptide
UDP + galactosyl-Muc1 peptide
-
synthetic substrate
-
?
UDP-galactose + Muc1 peptide
UDP + galactosyl-Muc1 peptide
-
2 of 3 threonine residues on the Muc1 tandem repeat peptides, none of the serine residues, maximum is 2 Mol of GalNAc linked to each 20-residue repeat unit
-
?
UDP-galactose + Muc2 peptide
UDP + galactosyl-Muc2 peptide
-
peptides derived of Muc2 mucin
-
-
?
UDP-galactose + Muc2 peptide
UDP + galactosyl-Muc2 peptide
-
only GalNAc-T2, only with the Muc2 acceptor peptide
-
-
?
UDP-galactose + Muc2 peptide
UDP + galactosyl-Muc2 peptide
-
investigation of preferences for distinct threonine residues in the peptide sequences
-
-
?
UDP-galactose + PTTTPITTTTK
UDP + galactosyl-PTTTPITTTTK
-
Muc2 peptide, investigation of preferences for distinct threonine residues in the peptide sequences of isozyme T1 to T4
-
-
?
UDP-GalNAc + EA2
GalNAc-EA2 + UDP
ternary complex of ppGalNAcT-2 in crystal structure PDB: 2FFU, UDP-GalNAc binding results in the closed confirmation of loop B which stabilises loop A by binding of loop A (N102) to loop B (R362), EA2 peptide binds in an extended confirmation
binary complex of UDP and ppGalNAcT-2 in crystal structure PDB: 2FFV
-
?
UDP-GalNAc + GAGA(X)5T(X)5AGAGK
GAGA(X)5(GalNAc)T(X)5AGAGK + UDP
transferase activity relatively insensitive to the nature of amino acids 4 and 5 residues away from site of glycosylation, high correlation between peptide preferences of PGANT5 and mammalian orthologue ppGalNAc T1 with r(square) = 0.67, the hydrophobic residue proline is preferred at the -1, +1, and +3 positions, the hydrophobic residues valine and tyrosin are moderately preferred at the -1 and +3 positions, the hydrophobic residue methionine is disfavoured at the -1 position, low hydrophilic preference at the -1 position, strong glutamate/aspartate preference at the +1 position, higher preference of glutamate at +1 and +2 positions than mammalian orthologue ppGalNAc T1
-
-
?
UDP-GalNAc + GAGA(X)5T(X)5AGAGK
GAGA(X)5(GalNAc)T(X)5AGAGK + UDP
transferase activity relatively insensitive to the nature of amino acids 4 and 5 residues away from site of glycosylation, very high correlation between peptide preferences of PGANT2 and mammalian orthologue ppGalNAc T2 with r(square) = 0.92
-
-
?
UDP-GalNAc + GAGA(X)5T(X)5AGAGK
UDP + GAGA(X)5(GalNAc)T(X)5AGAGK
very high correlation between peptide preferences of ppGalNAc T2 and fly orthologue PGANT2 with r(square) = 0.92, the hydrophobic residue proline is preferred at the -1, +1, and +3 positions, the hydrophobic residues valine and tyrosin are moderately preferred at the -1 and +3 positions, the hydrophobic residue methionine is disfavoured at the -1 position, low hydrophilic preference at the -1 position, strong glutamate/aspartate preference at the +1 position, lower preference of glutamate at +1 and +2 positions than fly orthologue PGANT5
-
-
?
UDP-GalNAc + GAGA(X)5T(X)5AGAGK
UDP + GAGA(X)5(GalNAc)T(X)5AGAGK
very high correlation between peptide preferences of ppGalNAc T2 and fly orthologue PGANT2 with r(square) = 0.92
-
-
?
UDP-GalNAc + GAGA(X)nT(X)nAGAGK
GAGA(X)n(GalNAc)T(X)nAGAGK + UDP
library of unmodified peptides, with randomized residue at position X and n=3 or n=5, random peptide preferences are not significantly altered by increased peptide substrate length n=5 compared to n=3 or the proximity of flanking Gly and Ala residues to the site of glycosylation, major peptide substrate preference determinants are the 2 to 3 residues flanking the site of glycosylation
-
-
?
UDP-GalNAc + GAGA(X)nT(X)nAGAGK
GAGA(X)n(GalNAc)T(X)nAGAGK + UDP
library of unmodified peptides, with randomized residue at position X and n=3 or n=5
-
-
?
UDP-GalNAc + GAGA(X)nT(X)nAGAGK
GAGA(X)n(GalNAc)T(X)nAGAGK + UDP
library of unmodified peptides, with randomized residue at position X and n=3 or n=5, random peptide preferences are not significantly altered by increased peptide substrate length n=5 compared to n=3 or the proximity of flanking Gly and Ala residues to the site of glycosylation, major peptide substrate preference determinants are the 2 to 3 residues flanking the site of glycosylation
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + polypeptide
UDP + N-acetyl-alpha-D-galactosaminyl-polypeptide
-
acceptor sites with adjacent proline residues are highly preferred, while other residues cause less pronounced effects. The enzyme clearly prefers threonine over serine in the in vitro assays
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + polypeptide
UDP + N-acetyl-alpha-D-galactosaminyl-polypeptide
-
-
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + polypeptide biotin-EA2
UDP + N-acetyl-alpha-D-galactosaminyl-polypeptide biotin-EA2
-
sequence biotin-PTTDSTTPAPTTK, second highest activity
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + polypeptide biotin-EA2
UDP + N-acetyl-alpha-D-galactosaminyl-polypeptide biotin-EA2
-
sequence biotin-PTTDSTTPAPTTK, second highest activity
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + polypeptide biotin-EA2
UDP + N-acetyl-alpha-D-galactosaminyl-polypeptide biotin-EA2
-
sequence biotin-PTTDSTTPAPTTK, second highest activity
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + polypeptide biotin-EA2-2
UDP + N-acetyl-alpha-D-galactosaminyl-polypeptide biotin-EA2-2
-
sequence biotin-PTTDSTTPAPTTK
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + polypeptide biotin-EA2-2
UDP + N-acetyl-alpha-D-galactosaminyl-polypeptide biotin-EA2-2
-
sequence biotin-PTTDSTTPAPTTK
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + polypeptide biotin-EA2-2
UDP + N-acetyl-alpha-D-galactosaminyl-polypeptide biotin-EA2-2
-
sequence biotin-PTTDSTTPAPTTK
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + polypeptide EA2
UDP + N-acetyl-alpha-D-galactosaminyl-polypeptide EA2
sequence PTTDSTTPAPTTK
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + polypeptide EA2
UDP + N-acetyl-alpha-D-galactosaminyl-polypeptide EA2
-
-
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + polypeptide EA2-biotin
UDP + N-acetyl-alpha-D-galactosaminyl-polypeptide EA2-biotin
-
sequence PTTDSTTPAPTTK-biotin
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + polypeptide EA2-biotin
UDP + N-acetyl-alpha-D-galactosaminyl-polypeptide EA2-biotin
-
sequence PTTDSTTPAPTTK-biotin
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + polypeptide EA2-biotin
UDP + N-acetyl-alpha-D-galactosaminyl-polypeptide EA2-biotin
-
sequence PTTDSTTPAPTTK-biotin
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + polypeptide GPIV
UDP + N-acetyl-alpha-D-galactosaminyl-polypeptide GPIV
-
preferred substrate
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + polypeptide GPIV
UDP + N-acetyl-alpha-D-galactosaminyl-polypeptide GPIV
-
preferred substrate for isoforms ppGalNAc T2, T5, T13, T14, and T16
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + polypeptide GPV
UDP + N-acetyl-alpha-D-galactosaminyl-polypeptide GPV
-
-
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + polypeptide GPV
UDP + N-acetyl-alpha-D-galactosaminyl-polypeptide GPV
-
preferred substrate for isoforms ppGalNAc T3, T5, T6, T13, and T16
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + polypeptide GPV-C
UDP + N-acetyl-alpha-D-galactosaminyl-polypeptide GPV-C
-
-
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + polypeptide GPV-C
UDP + N-acetyl-alpha-D-galactosaminyl-polypeptide GPV-C
-
-
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + polypeptide GRA2-biotin
UDP + N-acetyl-alpha-D-galactosaminyl-polypeptide GRA2-biotin
-
highest activity
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + polypeptide GRA2-biotin
UDP + N-acetyl-alpha-D-galactosaminyl-polypeptide GRA2-biotin
-
highest activity
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + polypeptide GRA2-biotin
UDP + N-acetyl-alpha-D-galactosaminyl-polypeptide GRA2-biotin
-
highest activity
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + polypeptide Muc2
UDP + N-acetyl-alpha-D-galactosaminyl-polypeptide Muc2
-
best acceptor polypeptide substrate with sequence PTTTPITTTTTVTPTPTPTGTQTK
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + polypeptide Muc2
UDP + N-acetyl-alpha-D-galactosaminyl-polypeptide Muc2
-
best substrate with sequence PTTTPITTTTTVTPTPTPTGTQTK
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + polypeptide Muc5Ac
UDP + N-acetyl-alpha-D-galactosaminyl-polypeptide Muc5Ac
-
second best acceptor polypeptide with sequence GTTPSPVPTTSTTSAP
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + polypeptide Muc5Ac
UDP + N-acetyl-alpha-D-galactosaminyl-polypeptide Muc5Ac
-
sequence GTTPSPVPTTSTTSAP
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + polypeptide Muc5Ac
UDP + N-acetyl-alpha-D-galactosaminyl-polypeptide Muc5Ac
-
-
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + polypeptide MUC5AC-3,13-biotin
UDP + N-acetyl-alpha-D-galactosaminyl-polypeptide MUC5AC-3,13-biotin
-
sequence biotin-GTTPSPVPTTSTTSAP
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + polypeptide MUC5AC-3,13-biotin
UDP + N-acetyl-alpha-D-galactosaminyl-polypeptide MUC5AC-3,13-biotin
-
sequence biotin-GTTPSPVPTTSTTSAP
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + polypeptide MUC5AC-3,13-biotin
UDP + N-acetyl-alpha-D-galactosaminyl-polypeptide MUC5AC-3,13-biotin
-
sequence biotin-GTTPSPVPTTSTTSAP
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + Pro-Pro-Asp-Ala-Ala-Ser-Ala-Ala-Pro-Leu-Arg
?
-
-
-
-
?
UDP-N-acetyl-alpha-D-galactosamine + Pro-Pro-Asp-Ala-Ala-Ser-Ala-Ala-Pro-Leu-Arg
?
-
-
-
-
?
UDP-N-acetyl-D-galactosamine + Ac-QATEYEYLDYDFLPETEPPEM
UDP + N-acetyl-D-galactosaminylated Ac-QATEYEYLDYDFLPETEPPEM
-
-
-
?
UDP-N-acetyl-D-galactosamine + Ac-QATEYEYLDYDFLPETEPPEM
UDP + N-acetyl-D-galactosaminylated Ac-QATEYEYLDYDFLPETEPPEM
acceptor substrate
-
-
?
UDP-N-acetyl-D-galactosamine + AHGVVTSAPDTR
UDP + ?
i.e. Muc1, 3% of the activity with Muc5AC as substrate, enzyme form pp-GalNAc-T2
-
-
?
UDP-N-acetyl-D-galactosamine + AHGVVTSAPDTR
UDP + ?
i.e. Muc1a, enzyme form pp-GalNAc-T15
-
-
?
UDP-N-acetyl-D-galactosamine + ANTPSFPTATSPAPPI
UDP + ?
i.e. Muc13, about 25% of the activity with Muc5AC as substrate, enzyme form pp-GalNAc-T2
-
-
?
UDP-N-acetyl-D-galactosamine + ANTPSFPTATSPAPPI
UDP + ?
i.e. Muc13, about 50% of the activity with Muc1a, enzyme form pp-GalNAc-T15
-
-
?
UDP-N-acetyl-D-galactosamine + apomucin
UDP + N-acetyl-D-galactosaminyl-apomucin
-
bovine apomucin
-
-
?
UDP-N-acetyl-D-galactosamine + apomucin
UDP + N-acetyl-D-galactosaminyl-apomucin
-
deglycosylated bovine submaxillary mucin
-
-
?
UDP-N-acetyl-D-galactosamine + apomucin
UDP + N-acetyl-D-galactosaminyl-apomucin
-
best substrate, poorer substrates: asialo mucin, native mucin
-
-
?
UDP-N-acetyl-D-galactosamine + apomucin
UDP + N-acetyl-D-galactosaminyl-apomucin
-
acceptor: apomucin motif encoded by the MUC5AC gene
-
-
?
UDP-N-acetyl-D-galactosamine + apomucin
UDP + N-acetyl-D-galactosaminyl-apomucin
-
enzymatically prepared apomucin accepts GalNAc much faster than chemically prepared apomucin
-
-
?
UDP-N-acetyl-D-galactosamine + apomucin
UDP + N-acetyl-D-galactosaminyl-apomucin
-
deglycosylated bovine submaxillary mucin
-
-
?
UDP-N-acetyl-D-galactosamine + apomucin
UDP + N-acetyl-D-galactosaminyl-apomucin
-
GalNAc-T1
-
-
?
UDP-N-acetyl-D-galactosamine + apomucin
UDP + N-acetyl-D-galactosaminyl-apomucin
-
deglycosylated bovine submaxillary mucin
-
-
?
UDP-N-acetyl-D-galactosamine + apomucin
UDP + N-acetyl-D-galactosaminyl-apomucin
-
porcine apomucin
-
-
?
UDP-N-acetyl-D-galactosamine + apomucin
UDP + N-acetyl-D-galactosaminyl-apomucin
-
ovine apomucin
-
-
?
UDP-N-acetyl-D-galactosamine + apomucin
UDP + N-acetyl-D-galactosaminyl-apomucin
-
deglycosylated Cowper's gland, swine and human trachea mucins
-
?
UDP-N-acetyl-D-galactosamine + CPPTPSATTPAPPSSSAPPETTAA
UDP + N-acetyl-D-galactosaminylated CPPTPSATTPAPPSSSAPPETTAA
mucin derived substrate
-
-
?
UDP-N-acetyl-D-galactosamine + CPPTPSATTPAPPSSSAPPETTAA
UDP + N-acetyl-D-galactosaminylated CPPTPSATTPAPPSSSAPPETTAA
mucin derived substrate, acceptor substrate
-
-
?
UDP-N-acetyl-D-galactosamine + GalNAc-glycosylated peptide
UDP + N-acetyl-D-galactosaminyl-GalNAc-glucosylated peptide
acceptor: MUC5AC-13 glycopeptide
-
-
?
UDP-N-acetyl-D-galactosamine + GalNAc-glycosylated peptide
UDP + N-acetyl-D-galactosaminyl-GalNAc-glucosylated peptide
GalNAc-T7: exclusive specificity for partially GalNAc-glycosylated acceptor substrates, GalNAc-glycosylated peptide substrates derived from the tandem repeats of human Muc2 and rat submandibular gland mucin, specificity of GalNAc-T4
-
-
?
UDP-N-acetyl-D-galactosamine + GalNAc-glycosylated peptide
UDP + N-acetyl-D-galactosaminyl-GalNAc-glucosylated peptide
-
rGalNAc-T4 is dependent on the presence of GalNAc on acceptor peptides, activity is triggered by a lectin-like interaction of enzyme with the glycopeptide directed by the putative lectin domain found in the C-terminal region, rGalNAc-T2 activity is enhanced on glycosylated acceptor peptides
-
-
?
UDP-N-acetyl-D-galactosamine + GalNAc-glycosylated peptide
UDP + N-acetyl-D-galactosaminyl-GalNAc-glucosylated peptide
-
ppGalNAc-T14 can utilize GalNAc transferred peptides as substrates
-
-
?
UDP-N-acetyl-D-galactosamine + GalNAc-glycosylated peptide
UDP + N-acetyl-D-galactosaminyl-GalNAc-glucosylated peptide
-
ppGalNAc-T4 and -T7 prefer peptides having GalNAc residues as acceptor substrates
-
-
?
UDP-N-acetyl-D-galactosamine + GalNAc-glycosylated peptide
UDP + N-acetyl-D-galactosaminyl-GalNAc-glucosylated peptide
-
acceptors: partially glycosylated versions of a peptide derived from mucin2, pp-GalNAc-T1, -T2, -T3 and -T4 exhibit distinct specificities toward glycopeptides, pathway of incorporation of the second and third GalNAc, isoenzymes may recognize directly GalNAc residues in the vicinity
-
-
?
UDP-N-acetyl-D-galactosamine + GalNAc-glycosylated peptide
UDP + N-acetyl-D-galactosaminyl-GalNAc-glucosylated peptide
ppGaNTase-T9: acceptors are di- and triglycosylated MUC5AC and mono-glycosylated EA2, obtained by prior action of ppGaNTase-T1, but not the unmodified peptides, formation of a tetra-glycopeptide species from MUC5AC triglycopeptide substrate, ppGaNTase-T7 forms a hexa-glycopeptide species
-
-
?
UDP-N-acetyl-D-galactosamine + GalNAc-glycosylated peptide
UDP + N-acetyl-D-galactosaminyl-GalNAc-glucosylated peptide
-
GalNAc-T1 may also specifically recognize and glycosylate partially glycosylated acceptors
-
-
?
UDP-N-acetyl-D-galactosamine + GalNAc-glycosylated peptide
UDP + N-acetyl-D-galactosaminyl-GalNAc-glucosylated peptide
ppGaNTase-T6 requires at least 2 intact GalNAc residues in the substrate, acceptor: MUC5AC-glycopeptide, obtained by the action of ppGaNTase-T1
-
-
?
UDP-N-acetyl-D-galactosamine + GTTPSPVPTTS-(O-GalNAc)T-SAP
UDP + ?
-
23% of the activity with P-(O-GalNAc)T-TDSTTPAPTTK
-
-
?
UDP-N-acetyl-D-galactosamine + GTTPSPVPTTS-(O-GalNAc)T-SAP
UDP + ?
-
-
-
-
?
UDP-N-acetyl-D-galactosamine + GTTPSPVPTTS-(O-GalNAc)T-SAP
UDP + ?
-
-
-
?
UDP-N-acetyl-D-galactosamine + GTTPSPVPTTSTTSAk
UDP + ?
i.e. Muc5AC, about 55% of the activity with Muc1a, pp-GalNAc-T15 shows significantly less catalytic activity than pp-GalNAc-T2,T15 transferrs up to seven GalNAcs to the Muc5AC peptide, while T2 transferrs up to five GalNAcs, enzyme form pp-GalNAc-T15
-
-
?
UDP-N-acetyl-D-galactosamine + GTTPSPVPTTSTTSAk
UDP + ?
i.e. Muc5AC, pp-GalNAc-T15 shows significantly less catalytic activity than pp-GalNAc-T2,T15 transferrs up to seven GalNAcs to the Muc5AC peptide, while T2 transferrs up to five GalNAcs
-
-
?
UDP-N-acetyl-D-galactosamine + GTTPSPVPTTSTTSAP
UDP + ?
i.e. MUC5AC
-
-
?
UDP-N-acetyl-D-galactosamine + GTTPSPVPTTSTTSAP
UDP + ?
i.e. MUC5AC, enzyme form PGANT4
-
-
?
UDP-N-acetyl-D-galactosamine + GTTPSPVPTTSTTSAP
UDP + ?
i.e. MUC5AC, low activity enzyme form PGANT6
-
-
?
UDP-N-acetyl-D-galactosamine + GTTPSPVPTTSTTSAP
UDP + ?
i.e. MUC5AC, low activity, enzyme form PGANT8
-
-
?
UDP-N-acetyl-D-galactosamine + GTTPSPVPTTSTTSAP
UDP + ?
i.e. MUC5AC, very low activity, enzyme form PGANT3
-
-
?
UDP-N-acetyl-D-galactosamine + GTTPSPVPTTSTTSAP
UDP + ?
-
73% of the activity with P-(O-GalNAc)T-TDSTTPAPTTK
-
-
?
UDP-N-acetyl-D-galactosamine + GTTPSPVPTTSTTSAP
UDP + ?
0.3% of the activity with GTTPSPVPTTS-(O-GalNAc)T-TSAP
-
-
?
UDP-N-acetyl-D-galactosamine + HIV-V3 peptide
UDP + N-acetyl-D-galactosaminyl-HIV-V3 peptide
-
HIVIIIBgp120, only GalNAc-T3
-
-
?
UDP-N-acetyl-D-galactosamine + HIV-V3 peptide
UDP + N-acetyl-D-galactosaminyl-HIV-V3 peptide
-
placenta, but not liver enzyme
-
-
?
UDP-N-acetyl-D-galactosamine + HIV-V3 peptide
UDP + N-acetyl-D-galactosaminyl-HIV-V3 peptide
-
submaxillary gland enzyme, low activity
-
-
?
UDP-N-acetyl-D-galactosamine + HIV-V3 peptide
UDP + N-acetyl-D-galactosaminyl-HIV-V3 peptide
-
submaxillary gland enzyme, low activity
-
-
?
UDP-N-acetyl-D-galactosamine + human chorionic gonadotropin-beta peptide
UDP + N-acetyl-D-galactosaminyl-human chorionic gonadotropin-beta peptide
-
placenta enzyme
-
-
?
UDP-N-acetyl-D-galactosamine + human chorionic gonadotropin-beta peptide
UDP + N-acetyl-D-galactosaminyl-human chorionic gonadotropin-beta peptide
-
only Ser acceptor sites, with a very low activity
-
-
?
UDP-N-acetyl-D-galactosamine + human chorionic gonadotropin-beta peptide
UDP + N-acetyl-D-galactosaminyl-human chorionic gonadotropin-beta peptide
-
only GalNAc-T2
-
-
?
UDP-N-acetyl-D-galactosamine + human chorionic gonadotropin-beta peptide
UDP + N-acetyl-D-galactosaminyl-human chorionic gonadotropin-beta peptide
-
only Ser acceptor sites, with a very low activity
-
-
?
UDP-N-acetyl-D-galactosamine + human chorionic gonadotropin-beta peptide
UDP + N-acetyl-D-galactosaminyl-human chorionic gonadotropin-beta peptide
-
submaxillary gland enzyme
-
-
?
UDP-N-acetyl-D-galactosamine + human chorionic gonadotropin-beta peptide
UDP + N-acetyl-D-galactosaminyl-human chorionic gonadotropin-beta peptide
-
only Ser acceptor sites, with a very low activity
-
-
?
UDP-N-acetyl-D-galactosamine + human chorionic gonadotropin-beta peptide
UDP + N-acetyl-D-galactosaminyl-human chorionic gonadotropin-beta peptide
-
submaxillary gland enzyme
-
-
?
UDP-N-acetyl-D-galactosamine + kappa-casein
UDP + N-acetyl-D-galactosaminyl-kappa-casein
-
kappa-subcomponent 1, the nonglycosylated kappa-casein, is the best substrate among kappa-casein subcomponents, asialo kappa-casein, agalacto kappa-casein and nature kappa-casein
-
-
?
UDP-N-acetyl-D-galactosamine + kappa-casein
UDP + N-acetyl-D-galactosaminyl-kappa-casein
-
glycosylation of kappa-casein occurs after casein micelle formation triggered by the accumulation of Ca2+ in vivo
-
-
?
UDP-N-acetyl-D-galactosamine + kappa-casein
UDP + N-acetyl-D-galactosaminyl-kappa-casein
-
-
-
-
?
UDP-N-acetyl-D-galactosamine + mucin MUC5AC-3
UDP + ?
enzyme form PGANT2
-
-
?
UDP-N-acetyl-D-galactosamine + mucin MUC5AC-3
UDP + ?
enzyme form PGANT4
-
-
?
UDP-N-acetyl-D-galactosamine + mucin MUC5AC-3
UDP + ?
enzyme form PGANT6
-
-
?
UDP-N-acetyl-D-galactosamine + mucin MUC5AC-3
UDP + ?
enzyme form PGANT7
-
-
?
UDP-N-acetyl-D-galactosamine + mucin MUC5AC-3
UDP + ?
enzyme form PGANT8
-
-
?
UDP-N-acetyl-D-galactosamine + mucin MUC5AC-3
UDP + ?
high activity, enzyme form PGANT5
-
-
?
UDP-N-acetyl-D-galactosamine + mucin MUC5AC-3
UDP + ?
PGANT1
-
-
?
UDP-N-acetyl-D-galactosamine + mucin MUC5AC-3
UDP + ?
very low activity, enzyme form PGANT3
-
-
?
UDP-N-acetyl-D-galactosamine + mucin MUC5AC-3/13
UDP + ?
enzyme form PGANT8
-
-
?
UDP-N-acetyl-D-galactosamine + mucin MUC5AC-3/13
UDP + ?
high activity, enzyme form PGANT4
-
-
?
UDP-N-acetyl-D-galactosamine + mucin MUC5AC-3/13
UDP + ?
high activity, enzyme form PGANT5
-
-
?
UDP-N-acetyl-D-galactosamine + mucin MUC5AC-3/13
UDP + ?
high activity, enzyme form PGANT7
-
-
?
UDP-N-acetyl-D-galactosamine + mucin MUC5AC-3/13
UDP + ?
low activity, enzyme form PGANT2
-
-
?
UDP-N-acetyl-D-galactosamine + mucin MUC5AC-3/13
UDP + ?
preferred substrate, enzyme form PGANT4
-
-
?
UDP-N-acetyl-D-galactosamine + mucin MUC5AX-13
UDP + ?
enzyme form PGANT4
-
-
?
UDP-N-acetyl-D-galactosamine + mucin MUC5AX-13
UDP + ?
enzyme form PGANT6
-
-
?
UDP-N-acetyl-D-galactosamine + mucin MUC5AX-13
UDP + ?
enzyme form PGANT8
-
-
?
UDP-N-acetyl-D-galactosamine + mucin MUC5AX-13
UDP + ?
high activity, enzyme form PGANT5
-
-
?
UDP-N-acetyl-D-galactosamine + mucin MUC5AX-13
UDP + ?
low activity, enzyme form PGANT7
-
-
?
UDP-N-acetyl-D-galactosamine + myelin basic protein
UDP + N-acetyl-D-galactosaminyl-myelin basic protein
-
-
-
-
?
UDP-N-acetyl-D-galactosamine + myelin basic protein
UDP + N-acetyl-D-galactosaminyl-myelin basic protein
-
-
-
-
?
UDP-N-acetyl-D-galactosamine + osteopontin
UDP + N-acetyl-D-galactosaminyl-osteopontin
-
isozyme ppGalNAc T10 prefers previously glycosylated peptides as substrates, ppGalNAc T10 exhibits a single large preference for Ser/Thr-O-GalNAc at the +1, C-terminal, position relative to the Ser or Thr acceptor site
-
-
?
UDP-N-acetyl-D-galactosamine + osteopontin
UDP + N-acetyl-D-galactosaminyl-osteopontin
-
preferred substrate of isozyme ppGalNAcT-1, glycosylation pattern, overview
-
-
?
UDP-N-acetyl-D-galactosamine + P-(O-GalNAc)T-TDSTTPAPTTK
UDP + ?
0.3% of the activity with GTTPSPVPTTS-(O-GalNAc)T-TSAP
-
-
?
UDP-N-acetyl-D-galactosamine + PDTRPAPGSTAPPAC
UDP + ?
i.e. MUC1b
-
-
?
UDP-N-acetyl-D-galactosamine + PDTRPAPGSTAPPAC
UDP + ?
i.e. MUC1b, enzyme form PGANT4
-
-
?
UDP-N-acetyl-D-galactosamine + PDTRPAPGSTAPPAC
UDP + ?
i.e. MUC1b, low activity, enzyme form PGANT2
-
-
?
UDP-N-acetyl-D-galactosamine + PDTRPAPGSTAPPAC
UDP + ?
i.e. MUC1b, low activity, enzyme form PGANT6
-
-
?
UDP-N-acetyl-D-galactosamine + PDTRPAPGSTAPPAC
UDP + ?
i.e. MUC1b, low activity, enzyme form PGANT8
-
-
?
UDP-N-acetyl-D-galactosamine + polypeptide
UDP + N-acetyl-D-galactosaminyl-polypeptide
-
-
-
-
?
UDP-N-acetyl-D-galactosamine + polypeptide
UDP + N-acetyl-D-galactosaminyl-polypeptide
-
acceptors: synthetic glycopeptides and peptides most of which contain sequences derived from the tandem repeat region of MUC2 mucin
-
-
?
UDP-N-acetyl-D-galactosamine + polypeptide
UDP + N-acetyl-D-galactosaminyl-polypeptide
-
acceptor: asialo mucin, subcomponents of kappa-casein, asialo kappa-casein, agalacto kappa-casein
-
-
?
UDP-N-acetyl-D-galactosamine + polypeptide
UDP + N-acetyl-D-galactosaminyl-polypeptide
-
acceptor: deglycosylated bovine submaxillary mucin
-
-
?
UDP-N-acetyl-D-galactosamine + polypeptide
UDP + N-acetyl-D-galactosaminyl-polypeptide
-
octapeptide Val-Thr-Pro-Arg-Thr-Pro-Pro-Pro is the best substrate, threonine cannot be glycosylated without a carboxyl triprolyl sequence, the alpha-amino acid group of the threonine must be blocked, the nature of the group NH2-terminal to the threonine affects the kinetics of the reaction, one residue can be between the threonine and the triprolyl sequence
-
-
?
UDP-N-acetyl-D-galactosamine + polypeptide
UDP + N-acetyl-D-galactosaminyl-polypeptide
-
rate of incorporation of GalNAc into Thr is significantly greater than into Ser residues, presence of 1-2 GalNAc-Thr moieties in the subtrate reduces enzyme activity, especially when Galbeta1-3GalNAc is present, O-glycosylation depends on both amino acid sequence and prior glycosylation of substrates, enzyme is selective in glycosylating peptides with the Pro-Thr-Thr-Thr-Pro-Ile-Ser-Thr sequence in that the preferred primary site is the third Thr from the N-terminal end
-
-
?
UDP-N-acetyl-D-galactosamine + polypeptide
UDP + N-acetyl-D-galactosaminyl-polypeptide
-
broad specificity, acceptors: unglycosylated protein acceptors, synthetic peptides, O-glycosylates threonine about 35times faster than serine, specificity is determined by the amino acids in the acceptor peptide segment and its accessibility, enzymatic active site interacts with an 8-amino acid long segment of the substrate, spanning 3 amino acids preceding and 4 amino acids following the reactive Thr or Ser
-
-
?
UDP-N-acetyl-D-galactosamine + polypeptide
UDP + N-acetyl-D-galactosaminyl-polypeptide
-
only UDP-GalNAc serves as sugar donor
-
-
?
UDP-N-acetyl-D-galactosamine + polypeptide
UDP + N-acetyl-D-galactosaminyl-polypeptide
-
acceptors: synthetic peptides
-
-
?
UDP-N-acetyl-D-galactosamine + polypeptide
UDP + N-acetyl-D-galactosaminyl-polypeptide
-
acceptors: apomucin, kappa-casein
-
-
?
UDP-N-acetyl-D-galactosamine + polypeptide
UDP + N-acetyl-D-galactosaminyl-polypeptide
-
O-glycosylating enzyme, acceptors: synthetic peptides in which the Thr-Pro-Pro-Pro sequence is varied
-
-
?
UDP-N-acetyl-D-galactosamine + polypeptide
UDP + N-acetyl-D-galactosaminyl-polypeptide
-
transfer of GalNAc to a serine or threonine residue on the acceptor protein
-
-
?
UDP-N-acetyl-D-galactosamine + polypeptide
UDP + N-acetyl-D-galactosaminyl-polypeptide
-
key role in O-linked glycosylation, catalyzes first step in the assembly
-
-
?
UDP-N-acetyl-D-galactosamine + polypeptide
UDP + N-acetyl-D-galactosaminyl-polypeptide
-
catalyzes the first step in biosynthesis of O-linked oligosaccharides in many glycoproteins
-
-
?
UDP-N-acetyl-D-galactosamine + polypeptide
UDP + N-acetyl-D-galactosaminyl-polypeptide
-
catalyzes in vivo glycosylation of both threonine and serine
-
-
?
UDP-N-acetyl-D-galactosamine + polypeptide
UDP + N-acetyl-D-galactosaminyl-polypeptide
-
initial step of mucin-type O-glycosylation
-
-
?
UDP-N-acetyl-D-galactosamine + polypeptide
UDP + N-acetyl-D-galactosaminyl-polypeptide
-
post-translational, initial reaction in O-linked oligosaccharide biosynthesis
-
-
?
UDP-N-acetyl-D-galactosamine + polypeptide
UDP + N-acetyl-D-galactosaminyl-polypeptide
mucin-type O-linked glycosylation of serine or threonine residues
-
-
?
UDP-N-acetyl-D-galactosamine + polypeptide
UDP + N-acetyl-D-galactosaminyl-polypeptide
-
usage of random peptide substrates, overview. Isozyme substrate specificity, amino acid residue enhancement factors for the ppGalNAc T isozyme are obtained from random peptide substrates, detailed overview
product analysis by NMR and mass spectrometry
-
?
UDP-N-acetyl-D-galactosamine + polypeptide
UDP + N-acetyl-D-galactosaminyl-polypeptide
-
-
-
-
?
UDP-N-acetyl-D-galactosamine + polypeptide
UDP + N-acetyl-D-galactosaminyl-polypeptide
acceptors: substantial activity only with EA2 peptide, less active with IgAh peptide
-
-
?
UDP-N-acetyl-D-galactosamine + polypeptide
UDP + N-acetyl-D-galactosaminyl-polypeptide
transfer of GalNAc to a serine or threonine residue on the acceptor protein
-
-
?
UDP-N-acetyl-D-galactosamine + polypeptide
UDP + N-acetyl-D-galactosaminyl-polypeptide
mucin-type O-linked glycosylation of serine or threonine residues
-
-
?
UDP-N-acetyl-D-galactosamine + polypeptide
UDP + N-acetyl-D-galactosaminyl-polypeptide
mucin-type O-linked glycosylation of serine or threonine residues
-
-
?
UDP-N-acetyl-D-galactosamine + polypeptide
UDP + N-acetyl-D-galactosaminyl-polypeptide
Drosophila sp. (in: flies)
-
glycosylates peptides containing a sequence of XTPXP or TTAAP most efficiently
-
-
?
UDP-N-acetyl-D-galactosamine + polypeptide
UDP + N-acetyl-D-galactosaminyl-polypeptide
-
can participate in the biosynthesis of O-glycosylated parasite proteins exposed at the interface between Echinococcus granulosus and its hosts
-
-
?
UDP-N-acetyl-D-galactosamine + polypeptide
UDP + N-acetyl-D-galactosaminyl-polypeptide
-
transfers GalNAc to a broad range of substrate peptides, derived from human mucins and O-glycosylated parasite proteins, including acceptors containing only serine or only threonine residues
-
-
?
UDP-N-acetyl-D-galactosamine + polypeptide
UDP + N-acetyl-D-galactosaminyl-polypeptide
-
-
-
-
?
UDP-N-acetyl-D-galactosamine + polypeptide
UDP + N-acetyl-D-galactosaminyl-polypeptide
-
-
-
?
UDP-N-acetyl-D-galactosamine + polypeptide
UDP + N-acetyl-D-galactosaminyl-polypeptide
-
-
-
?
UDP-N-acetyl-D-galactosamine + polypeptide
UDP + N-acetyl-D-galactosaminyl-polypeptide
-
ppGalNAc-T14: acceptors are mucin-derived peptides such as Muc2, Muc5AC, Muc7, Muc13
-
-
?
UDP-N-acetyl-D-galactosamine + polypeptide
UDP + N-acetyl-D-galactosaminyl-polypeptide
ppGalNAc-T13: acceptors are mucin peptides, such as Muc1a', Muc5Ac and Muc7, forms a triplet Tn epitope, 3 consecutive GalNAc-Ser/Thr structures, on peptides encoded in syndecan-3, different substrate specificities of ppGalNAc-T1 and -T13
-
-
?
UDP-N-acetyl-D-galactosamine + polypeptide
UDP + N-acetyl-D-galactosaminyl-polypeptide
-
substrate specificities of recombinant ppGalNAc-T1, -T2, -T3, -T4, -T6 and T9
-
-
?
UDP-N-acetyl-D-galactosamine + polypeptide
UDP + N-acetyl-D-galactosaminyl-polypeptide
-
site-specific O-glycosylation by rGalNAc-T2 and -T4 is controlled by the primary sequence context and the position and structure of previously introduced O-glycans, acceptors: synthetic mucin-type peptides and glycopeptides derived from tandem repeat regions of MUC1, MUC2 and MUC4, minimum peptide length is required, which is larger for glycosylated substrates, concerted and sequential action of rGalNAc-T2 and -T4 fully glycosylates MUC1, but only partially MUC2 and MUC4 tandem repeat peptides
-
-
?
UDP-N-acetyl-D-galactosamine + polypeptide
UDP + N-acetyl-D-galactosaminyl-polypeptide
-
acceptor specificity
-
?
UDP-N-acetyl-D-galactosamine + polypeptide
UDP + N-acetyl-D-galactosaminyl-polypeptide
-
GalNAc-T1 and -T3: strict specificity for UDP-GalNAc, GalNAc-T2: utilizes UDP-GalNAc and UDP-Gal
-
-
?
UDP-N-acetyl-D-galactosamine + polypeptide
UDP + N-acetyl-D-galactosaminyl-polypeptide
-
acceptor: mucin2 derived fluorescein-conjugated peptide Pro-Thr-Thr-Thr-Pro-Leu-Lys, preferential sites of O-glycosylation by pp-GalNAc-T1, -T2, -T3 and -T4, of which each has a unique specificity
-
-
?
UDP-N-acetyl-D-galactosamine + polypeptide
UDP + N-acetyl-D-galactosaminyl-polypeptide
-
acceptor: human MUC5AC peptide Thr-Thr-Ser-Ala-Pro-Thr-Thr-Ser
-
-
?
UDP-N-acetyl-D-galactosamine + polypeptide
UDP + N-acetyl-D-galactosaminyl-polypeptide
-
transfer of GalNAc to a serine or threonine residue on the acceptor protein
-
-
?
UDP-N-acetyl-D-galactosamine + polypeptide
UDP + N-acetyl-D-galactosaminyl-polypeptide
-
acceptors: human mucin Muc1 and Muc2 peptides, human chorionic gonadotropin-beta peptide, HIV-V3 peptides
-
-
?
UDP-N-acetyl-D-galactosamine + polypeptide
UDP + N-acetyl-D-galactosaminyl-polypeptide
-
3 GalNAc-transferases: GalNAc-T1, -T2 and -T3 with distinct but partly overlapping specificities, dependent on the primary sequence of the acceptor substrate, acceptors: peptides based on Muc1 tandem repeat, TAP24, Muc2, Muc5AC, erythropoietin, hCG-beta, HIVIIIBgp120, ovine submaxillary mucin fragment, peptide derived from fibronectin, GalNAc-T1, -T2 and -T3 react at different rates with individual sites in the Muc1 repeat
-
-
?
UDP-N-acetyl-D-galactosamine + polypeptide
UDP + N-acetyl-D-galactosaminyl-polypeptide
ppGalNAc-T13: synthesis of O-glycan, specifically the Tn antigen in neurons
-
-
?
UDP-N-acetyl-D-galactosamine + polypeptide
UDP + N-acetyl-D-galactosaminyl-polypeptide
GalNAc-T7: functions as a follow-up enzyme in the initiation step of O-glycosylation
-
-
?
UDP-N-acetyl-D-galactosamine + polypeptide
UDP + N-acetyl-D-galactosaminyl-polypeptide
-
initiation of mucin-type O-glycosylation by a family of polypeptide GalNAc-transferases, of which each has a unique function
-
-
?
UDP-N-acetyl-D-galactosamine + polypeptide
UDP + N-acetyl-D-galactosaminyl-polypeptide
initiation of mucin-type O-glycosylation by a family of polypeptide GalNAc-transferases, of which each has a unique function
-
-
?
UDP-N-acetyl-D-galactosamine + polypeptide
UDP + N-acetyl-D-galactosaminyl-polypeptide
-
different enzyme activities are involved in the initiation of GalNAc O-glycosylation and these are differentially expressed in cells and organs
-
-
?
UDP-N-acetyl-D-galactosamine + polypeptide
UDP + N-acetyl-D-galactosaminyl-polypeptide
-
ppGalNAc-T14 may be involved in O-glycosylation in kidney
-
-
?
UDP-N-acetyl-D-galactosamine + polypeptide
UDP + N-acetyl-D-galactosaminyl-polypeptide
-
catalyzes the first step in biosynthesis of O-linked oligosaccharides in many glycoproteins
-
-
?
UDP-N-acetyl-D-galactosamine + polypeptide
UDP + N-acetyl-D-galactosaminyl-polypeptide
-
GalNAc-T9 catalyzes O-glycosylation in brain
-
-
?
UDP-N-acetyl-D-galactosamine + polypeptide
UDP + N-acetyl-D-galactosaminyl-polypeptide
-
post-translational, initial reaction in O-linked oligosaccharide biosynthesis
-
-
?
UDP-N-acetyl-D-galactosamine + polypeptide
UDP + N-acetyl-D-galactosaminyl-polypeptide
-
ppGalNAc-T2: initiation of O-glycosylation in the IgA1 hinge region
-
-
?
UDP-N-acetyl-D-galactosamine + polypeptide
UDP + N-acetyl-D-galactosaminyl-polypeptide
-
primary step in the production of mucin-type O-linked oligosaccharide groups in glycoproteins
-
-
?
UDP-N-acetyl-D-galactosamine + polypeptide
UDP + N-acetyl-D-galactosaminyl-polypeptide
the enzyme is involved in the normal development of the brain through O-glycosylation of proteins in the neurons
-
-
?
UDP-N-acetyl-D-galactosamine + polypeptide
UDP + N-acetyl-D-galactosaminyl-polypeptide
-
mucin-type O-linked glycosylation of serine or threonine residues
-
-
?
UDP-N-acetyl-D-galactosamine + polypeptide
UDP + N-acetyl-D-galactosaminyl-polypeptide
mucin-type O-linked glycosylation of serine or threonine residues
-
-
?
UDP-N-acetyl-D-galactosamine + polypeptide
UDP + N-acetyl-D-galactosaminyl-polypeptide
mucin-type O-linked glycosylation of serine or threonine residues
-
-
?
UDP-N-acetyl-D-galactosamine + polypeptide
UDP + N-acetyl-D-galactosaminyl-polypeptide
mucin-type O-linked glycosylation of serine or threonine residues
-
-
?
UDP-N-acetyl-D-galactosamine + polypeptide
UDP + N-acetyl-D-galactosaminyl-polypeptide
mucin-type O-linked glycosylation of serine or threonine residues
-
-
?
UDP-N-acetyl-D-galactosamine + polypeptide
UDP + N-acetyl-D-galactosaminyl-polypeptide
-
usage of random peptide substrates, overview. Isozyme substrate specificities, amino acid residue enhancement factors for ppGalNAc T isozymes are obtained from random peptide substrates, detailed overview
product analysis by NMR and mass spectrometry
-
?
UDP-N-acetyl-D-galactosamine + polypeptide
UDP + N-acetyl-D-galactosaminyl-polypeptide
-
-
-
-
?
UDP-N-acetyl-D-galactosamine + polypeptide
UDP + N-acetyl-D-galactosaminyl-polypeptide
-
ppGaNTase-T1: EA2 peptide acceptor, interdependence of binding of UDP-GalNAc and acceptor substrate, enzyme undergoes a conformational change upon sugar binding, equilibrium between open and closed states may be used as a regulation mechanism of activity
-
-
?
UDP-N-acetyl-D-galactosamine + polypeptide
UDP + N-acetyl-D-galactosaminyl-polypeptide
-
only UDP-GalNAc serves as sugar donor
-
-
?
UDP-N-acetyl-D-galactosamine + polypeptide
UDP + N-acetyl-D-galactosaminyl-polypeptide
-
key role in O-linked glycosylation, catalyzes first step in the assembly
-
-
?
UDP-N-acetyl-D-galactosamine + polypeptide
UDP + N-acetyl-D-galactosaminyl-polypeptide
-
initiation of mucin-type O-glycosylation by a family of polypeptide GalNAc-transferases, of which each has a unique function
-
-
?
UDP-N-acetyl-D-galactosamine + polypeptide
UDP + N-acetyl-D-galactosaminyl-polypeptide
-
O-glycosylation by ppGaNTase-Ts of multisite substrates may proceed in a specific hierarchical manner
-
-
?
UDP-N-acetyl-D-galactosamine + polypeptide
UDP + N-acetyl-D-galactosaminyl-polypeptide
initiates the formation of mucin-type, O-linked glycans by catalyzing the transfer of alpha-N-acetylgalactosamine from UDP-GalNAc to Ser or Thr residues of core proteins to form the Tn antigen (GalNAc-alpha-1-O-Ser/Thr)
-
-
?
UDP-N-acetyl-D-galactosamine + polypeptide
UDP + N-acetyl-D-galactosaminyl-polypeptide
-
mucin-type O-linked glycosylation of serine or threonine residues
-
-
?
UDP-N-acetyl-D-galactosamine + polypeptide
UDP + N-acetyl-D-galactosaminyl-polypeptide
-
transfer of GalNAc to a serine or threonine residue on the acceptor protein
-
-
?
UDP-N-acetyl-D-galactosamine + polypeptide
UDP + N-acetyl-D-galactosaminyl-polypeptide
-
acceptors: human mucin Muc1 and Muc2 peptides, human chorionic gonadotropin-beta peptide, HIV-V3 peptides
-
-
?
UDP-N-acetyl-D-galactosamine + polypeptide
UDP + N-acetyl-D-galactosaminyl-polypeptide
-
GalNAc-T1: Asp-144 is the most important site for GalNAc recognition, primarily recognizes the UDP portion of UDP-GalNAc
-
-
?
UDP-N-acetyl-D-galactosamine + polypeptide
UDP + N-acetyl-D-galactosaminyl-polypeptide
-
only UDP-GalNAc serves as sugar donor
-
-
?
UDP-N-acetyl-D-galactosamine + polypeptide
UDP + N-acetyl-D-galactosaminyl-polypeptide
-
acceptors: apomucin, kappa-casein
-
-
?
UDP-N-acetyl-D-galactosamine + polypeptide
UDP + N-acetyl-D-galactosaminyl-polypeptide
-
acceptors: A1 protein, apofetuin, apoantifreeze glycoproteins, frequent glycosylation of the abundant serine plus threonine residues of mucins
-
-
?
UDP-N-acetyl-D-galactosamine + polypeptide
UDP + N-acetyl-D-galactosaminyl-polypeptide
ppGaNTase-T1: acceptor is MUC5AC peptide, di-glycopeptide contains GalNAc at Thr-3 and -13, tri-glycopeptide an additional one at Ser-5
-
-
?
UDP-N-acetyl-D-galactosamine + polypeptide
UDP + N-acetyl-D-galactosaminyl-polypeptide
-
transfer of GalNAc to a serine or threonine residue on the acceptor protein
-
-
?
UDP-N-acetyl-D-galactosamine + polypeptide
UDP + N-acetyl-D-galactosaminyl-polypeptide
transfer of GalNAc to a serine or threonine residue on the acceptor protein
-
-
?
UDP-N-acetyl-D-galactosamine + polypeptide
UDP + N-acetyl-D-galactosaminyl-polypeptide
diversely regulated ppGaNTase family may play a role in the various processes governing development
-
-
?
UDP-N-acetyl-D-galactosamine + polypeptide
UDP + N-acetyl-D-galactosaminyl-polypeptide
-
GalNAc-T1 glycosylates unmodified polypeptides in vivo
-
-
?
UDP-N-acetyl-D-galactosamine + polypeptide
UDP + N-acetyl-D-galactosaminyl-polypeptide
-
initiation of mucin-type O-glycosylation by a family of polypeptide GalNAc-transferases, of which each has a unique function
-
-
?
UDP-N-acetyl-D-galactosamine + polypeptide
UDP + N-acetyl-D-galactosaminyl-polypeptide
initiation of mucin-type O-glycosylation by a family of polypeptide GalNAc-transferases, of which each has a unique function
-
-
?
UDP-N-acetyl-D-galactosamine + polypeptide
UDP + N-acetyl-D-galactosaminyl-polypeptide
initiation of mucin-type O-glycosylation by a family of polypeptide GalNAc-transferases, of which each has a unique function
-
-
?
UDP-N-acetyl-D-galactosamine + polypeptide
UDP + N-acetyl-D-galactosaminyl-polypeptide
-
different enzyme activities are involved in the initiation of GalNAc O-glycosylation and these are differentially expressed in cells and organs
-
-
?
UDP-N-acetyl-D-galactosamine + polypeptide
UDP + N-acetyl-D-galactosaminyl-polypeptide
O-glycosylation by ppGaNTase-Ts of multisite substrates may proceed in a specific hierarchical manner
-
-
?
UDP-N-acetyl-D-galactosamine + polypeptide
UDP + N-acetyl-D-galactosaminyl-polypeptide
-
the enzyme is involved in the normal development of the brain through O-glycosylation of proteins in the neurons
-
-
?
UDP-N-acetyl-D-galactosamine + polypeptide
UDP + N-acetyl-D-galactosaminyl-polypeptide
mucin-type O-linked glycosylation of serine or threonine residues
-
-
?
UDP-N-acetyl-D-galactosamine + polypeptide
UDP + N-acetyl-D-galactosaminyl-polypeptide
-
-
-
-
?
UDP-N-acetyl-D-galactosamine + polypeptide
UDP + N-acetyl-D-galactosaminyl-polypeptide
-
L-threonine specifically O-glycosylated in alpha-configuration, Thr-Pro-Pro-Pro-sequence requires a peptide length of five or more for significant acceptor activity
-
-
?
UDP-N-acetyl-D-galactosamine + polypeptide
UDP + N-acetyl-D-galactosaminyl-polypeptide
-
only UDP-GalNAc serves as sugar donor
-
?
UDP-N-acetyl-D-galactosamine + polypeptide
UDP + N-acetyl-D-galactosaminyl-polypeptide
-
acceptors: synthetic polypeptides with sequences identical or similar to those found in porcine mucin or human erythropoietin, acceptor substrate specificity is dependent on the amino acid sequence adjacent to serine and threonine residues, but it is more sensitive to the sequence adjacent to serine than to threonine
-
-
?
UDP-N-acetyl-D-galactosamine + polypeptide
UDP + N-acetyl-D-galactosaminyl-polypeptide
-
acceptors: bovine submaxillary mucin core protein, myelin basic protein
-
-
?
UDP-N-acetyl-D-galactosamine + polypeptide
UDP + N-acetyl-D-galactosaminyl-polypeptide
-
O-glycosylation of the polypeptide, acceptor: porcine stomach mucin
-
-
?
UDP-N-acetyl-D-galactosamine + polypeptide
UDP + N-acetyl-D-galactosaminyl-polypeptide
-
acceptors: ovine and porcine apomucin, porcine mucin-like synthetic peptides
-
-
?
UDP-N-acetyl-D-galactosamine + polypeptide
UDP + N-acetyl-D-galactosaminyl-polypeptide
-
O-glycosylating enzyme, acceptors: synthetic peptides in which the Thr-Pro-Pro-Pro sequence is varied
-
-
?
UDP-N-acetyl-D-galactosamine + polypeptide
UDP + N-acetyl-D-galactosaminyl-polypeptide
-
acceptor: SIV3 peptide
-
-
?
UDP-N-acetyl-D-galactosamine + polypeptide
UDP + N-acetyl-D-galactosaminyl-polypeptide
-
transfer of GalNAc to a serine or threonine residue on the acceptor protein
-
?
UDP-N-acetyl-D-galactosamine + polypeptide
UDP + N-acetyl-D-galactosaminyl-polypeptide
-
transfer of GalNAc to a serine or threonine residue on the acceptor protein
-
-
?
UDP-N-acetyl-D-galactosamine + polypeptide
UDP + N-acetyl-D-galactosaminyl-polypeptide
-
acceptors: deglycosylated Cowper's gland, swine and human trachea mucins, tryptic peptides prepared from them, best substrates are fragments of incompletely deglycosylated mucin polypeptide chains
-
?
UDP-N-acetyl-D-galactosamine + polypeptide
UDP + N-acetyl-D-galactosaminyl-polypeptide
-
specifically transfers N-acetylgalactosamine from UDP-GalNAc to the hydroxyl group of threonine, no transferase activity towards serine-containing peptides, amino acid sequence adjacent to glycosylated Thr residues may influence the rate of glycosylation
-
-
?
UDP-N-acetyl-D-galactosamine + polypeptide
UDP + N-acetyl-D-galactosaminyl-polypeptide
-
acceptors: human mucin Muc1 and Muc2 peptides, human chorionic gonadotropin-beta peptide, HIV-V3 peptides
-
-
?
UDP-N-acetyl-D-galactosamine + polypeptide
UDP + N-acetyl-D-galactosaminyl-polypeptide
-
catalyzes the first step in biosynthesis of O-linked oligosaccharides in many glycoproteins
-
-
?
UDP-N-acetyl-D-galactosamine + polypeptide
UDP + N-acetyl-D-galactosaminyl-polypeptide
-
first step in biosynthesis of O-linked oligosaccharides in submaxillary gland
-
-
?
UDP-N-acetyl-D-galactosamine + polypeptide
UDP + N-acetyl-D-galactosaminyl-polypeptide
-
key role in overall control of O-glycosylation
-
-
?
UDP-N-acetyl-D-galactosamine + polypeptide
UDP + N-acetyl-D-galactosaminyl-polypeptide
-
primary step in the production of mucin-type O-linked oligosaccharide groups in glycoproteins
-
-
?
UDP-N-acetyl-D-galactosamine + polypeptide
UDP + N-acetyl-D-galactosaminyl-polypeptide
-
-
-
?
UDP-N-acetyl-D-galactosamine + polypeptide
UDP + N-acetyl-D-galactosaminyl-polypeptide
-
mucin-type O-linked glycosylation of serine or threonine residues
-
-
?
UDP-N-acetyl-D-galactosamine + PSTPPTPSPSTPPTPSPS
UDP + ?
i.e. IgAh
-
-
?
UDP-N-acetyl-D-galactosamine + PSTPPTPSPSTPPTPSPS
UDP + ?
i.e. IgAh, low activity, enzyme form PGANT2
-
-
?
UDP-N-acetyl-D-galactosamine + PSTPPTPSPSTPPTPSPS
UDP + ?
i.e. IgAh, low activity, enzyme form PGANT8
-
-
?
UDP-N-acetyl-D-galactosamine + PSTPPTPSPSTPPTPSPS
UDP + ?
i.e. IgAh, very low activity, enzyme form PGANT3
-
-
?
UDP-N-acetyl-D-galactosamine + PTTDS-(O-GalNAc)T-TPAPTTK
UDP + ?
-
4.5% of the activity with P-(O-GalNAc)T-TDSTTPAPTTK
-
-
?
UDP-N-acetyl-D-galactosamine + PTTDS-(O-GalNAc)T-TPAPTTK
UDP + ?
-
38.7% of the activity with GTTPSPVPTTS-(O-GalNAc)T-TSAP
-
-
?
UDP-N-acetyl-D-galactosamine + PTTDS-(O-GalNAc)T-TPAPTTK
UDP + ?
59.6% of the activity with GTTPSPVPTTS-(O-GalNAc)T-TSAP
-
-
?
UDP-N-acetyl-D-galactosamine + PTTDSTTPAPTT
UDP + ?
i.e. EA2, about 25% of the activity with Muc1a, enzyme form pp-GalNAc-T15
-
-
?
UDP-N-acetyl-D-galactosamine + PTTDSTTPAPTT
UDP + ?
i.e. EA2, about 35% of the activity with Muc5AC as substrate, enzyme form pp-GalNAc-T2
-
-
?
UDP-N-acetyl-D-galactosamine + PTTDSTTPAPTTK
UDP + ?
enzyme form PGANT2
-
-
?
UDP-N-acetyl-D-galactosamine + PTTDSTTPAPTTK
UDP + ?
enzyme form PGANT3
-
-
?
UDP-N-acetyl-D-galactosamine + PTTDSTTPAPTTK
UDP + ?
i.e. EA2, enzyme form PGANT4
-
-
?
UDP-N-acetyl-D-galactosamine + PTTDSTTPAPTTK
UDP + ?
i.e. EA2, enzyme form PGANT6
-
-
?
UDP-N-acetyl-D-galactosamine + PTTDSTTPAPTTK
UDP + ?
i.e. EA2, enzyme form PGANT8
-
-
?
UDP-N-acetyl-D-galactosamine + PTTDSTTPAPTTK
UDP + ?
i.e. EA2, glycosylation at threonines 7, 11, and 12, enzyme form PGANT1
-
-
?
UDP-N-acetyl-D-galactosamine + PTTDSTTPAPTTK
UDP + ?
i.e. EA2, high activity, glycosylation at threonines 7, 11, and 12, enzyme form PGANT1
-
-
?
UDP-N-acetyl-D-galactosamine + PTTDSTTPAPTTK
UDP + ?
-
74% of the activity with P-(O-GalNAc)T-TDSTTPAPTTK
-
-
?
UDP-N-acetyl-D-galactosamine + PTTDSTTPAPTTK
UDP + ?
enzyme form ppGaNTase-T1
-
-
?
UDP-N-acetyl-D-galactosamine + PTTDSTTPAPTTK
UDP + ?
enzyme form ppGaNTase-T3
-
-
?
UDP-N-acetyl-D-galactosamine + PTTDSTTPAPTTK
UDP + ?
enzyme form ppGaNTase-T4
-
-
?
UDP-N-acetyl-D-galactosamine + PTTDSTTPAPTTK
UDP + ?
enzyme form ppGaNTase-T5
-
-
?
UDP-N-acetyl-D-galactosamine + PTTDSTTPAPTTK
UDP + ?
0.3% of the activity with GTTPSPVPTTS-(O-GalNAc)T-TSAP
-
-
?
UDP-N-acetyl-D-galactosamine + PTTTPITTTTTV
UDP + ?
-
i.e. Muc2
-
-
?
UDP-N-acetyl-D-galactosamine + PTTTPITTTTTVTPTPTPTGTQT
UDP + ?
i.e. Muc2, about 35% of the activity with Muc1a, enzyme form pp-GalNAc-T15
-
-
?
UDP-N-acetyl-D-galactosamine + PTTTPITTTTTVTPTPTPTGTQT
UDP + ?
i.e. Muc2, about 95% of the activity with Muc5AC as substrate, enzyme form pp-GalNAc-T2
-
-
?
UDP-N-acetyl-D-galactosamine + SPTTSTPISSTPQPTS
UDP + ?
i.e. rMUC-2, enzyme form PGANT2
-
-
?
UDP-N-acetyl-D-galactosamine + SPTTSTPISSTPQPTS
UDP + ?
i.e. rMUC-2, enzyme form PGANT4
-
-
?
UDP-N-acetyl-D-galactosamine + SPTTSTPISSTPQPTS
UDP + ?
i.e. rMUC-2, high activity
-
-
?
UDP-N-acetyl-D-galactosamine + SPTTSTPISSTPQPTS
UDP + ?
i.e. rMUC-2, low activity. enzyme form PGANT6
-
-
?
UDP-N-acetyl-D-galactosamine + TAPPAHGVTSAPDTRPAPGSTAPP
UDP + N-acetyl-D-galactosaminylated TAPPAHGVTSAPDTRPAPGSTAPP
-
-
-
?
UDP-N-acetyl-D-galactosamine + TAPPAHGVTSAPDTRPAPGSTAPP
UDP + N-acetyl-D-galactosaminylated TAPPAHGVTSAPDTRPAPGSTAPP
acceptor substrate
-
-
?
UDP-N-acetyl-D-galactosamine + VESDRSTTTTQAP
UDP + ?
-
34% of the activity with P-(O-GalNAc)T-TDSTTPAPTTK
-
-
?
UDP-N-acetyl-D-galactosamine + VESDRSTTTTQAP
UDP + ?
0.3% of the activity with GTTPSPVPTTS-(O-GalNAc)T-TSAP
-
-
?
UDP-N-acetyl-D-galactosamine + VLTTTATTPTA
UDP + ?
-
i.e. Muc5B
-
-
?
additional information
?
-
-
no activity with UDP-alpha-D-galactose, UDP-N-acetyl-alpha-D-glucoamine, GDP-fucose, UDP-xylose, UDP-glucuronic acid and CMP-neuraminic acid
-
-
?
additional information
?
-
-
not: alphaS1-casein, alphaS2-casein, beta-casein, alpha-lactalbumin, beta-lactoglobulin, bovine serum albumin
-
-
?
additional information
?
-
-
UDP-GalNAc:polypeptide alpha-N-acetylgalactosaminyltransferases transfer GalNAc from UDP-GalNAc to the Ser and Thr residues of polypeptide acceptors, and initiate and regulate mucin-type O-glycosylation
-
-
?
additional information
?
-
-
almost no activity with polypeptides biotin-EA2-7, biotin-MUC5AC, and no activity with polypeptide MUC5AC-biotin
-
-
?
additional information
?
-
-
almost no activity with polypeptides biotin-EA2-7, biotin-MUC5AC, and no activity with polypeptide MUC5AC-biotin
-
-
?
additional information
?
-
-
almost no activity with polypeptides biotin-EA2-7, biotin-MUC5AC, and no activity with polypeptide MUC5AC-biotin
-
-
?
additional information
?
-
enzyme exhibits a strong preference for UDP-GalNAc over other nucleotide sugar donors, with no activity against UDP-Gal, and 40% activity of that seen toward UDP-GalNAc toward UDP-GlcNAc
-
-
-
additional information
?
-
-
enzyme exhibits a strong preference for UDP-GalNAc over other nucleotide sugar donors, with no activity against UDP-Gal, and 40% activity of that seen toward UDP-GalNAc toward UDP-GlcNAc
-
-
-
additional information
?
-
519-amino acid putative catalytic region
-
-
?
additional information
?
-
-
519-amino acid putative catalytic region
-
-
?
additional information
?
-
involved in development, necessary for viability
-
-
?
additional information
?
-
-
involved in development, necessary for viability
-
-
?
additional information
?
-
GalNAc-T7: no activity with non-glycosylated peptides and GalNAc-glycosylated Muc1 substrate
-
-
?
additional information
?
-
-
GalNAc-T7: no activity with non-glycosylated peptides and GalNAc-glycosylated Muc1 substrate
-
-
?
additional information
?
-
-
different substrate specificities before and after Muc2 affinity chromatography of enzyme
-
-
?
additional information
?
-
-
14 distinct isoforms different with regard to their expression and specificity, but overlaps also occur, ppGalNAc-T14 forms together with ppGalNAc-T2 a subfamily of ppGalNAc-T gene family
-
-
?
additional information
?
-
-
all known rGalNAc-Ts are characterized by a putative lectin domain in their C-terminal regions
-
-
?
additional information
?
-
-
GalNAc-T9 contains a 485-amino acid putative catalytic region, GalNAc-T1, -T2, -T4 and -T8 belongs to the housekeeping enzymes with ubiquitous expression, GalNAc-T3, -T5, -T6, -T7 and -T9 belongs to the tissue-specific enzymes
-
-
?
additional information
?
-
-
existence of multiple enzyme activities, differentially expressed in different organs, at least 2 distinct enzyme specificities
-
-
?
additional information
?
-
-
amino acid sequences of seven GalNAc-transferases
-
-
?
additional information
?
-
amino acid sequences of seven GalNAc-transferases
-
-
?
additional information
?
-
-
amino acid sequences of seven GalNAc-transferases
-
-
?
additional information
?
-
-
pp-GalNAc-T13 is specifically expressed in neurons and synthesizes Tn antigen
-
-
?
additional information
?
-
pp-GalNAc-T13 is specifically expressed in neurons and synthesizes Tn antigen
-
-
?
additional information
?
-
MUC1-2GalNAc, MUC2-6GalNAc, OSM fragments, hCG-beta are not substrates for GalNAc-T11
-
-
?
additional information
?
-
MUC1-2GalNAc, MUC2-6GalNAc, OSM fragments, hCG-beta are not substrates for GalNAc-T11
-
-
?
additional information
?
-
MUC1-2GalNAc, MUC2-6GalNAc, OSM fragments, hCG-beta are not substrates for GalNAc-T11
-
-
?
additional information
?
-
MUC1-2GalNAc, MUC2-6GalNAc, OSM fragments, hCG-beta are not substrates for GalNAc-T11
-
-
?
additional information
?
-
MUC1b' and hCG-beta are no substrates for GalNAc-T1
-
-
?
additional information
?
-
MUC1b' and hCG-beta are no substrates for GalNAc-T1
-
-
?
additional information
?
-
MUC1b' and hCG-beta are no substrates for GalNAc-T1
-
-
?
additional information
?
-
MUC1b' and hCG-beta are no substrates for GalNAc-T1
-
-
?
additional information
?
-
-
UDP-GalNAc:polypeptide alpha-N-acetylgalactosaminyltransferases transfer GalNAc from UDP-GalNAc to the Ser and Thr residues of polypeptide acceptors, and initiate and regulate mucin-type O-glycosylation
-
-
?
additional information
?
-
-
most active with partially glycosylated MUC5AC (by a combination of UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 1 and UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase 2)
-
-
?
additional information
?
-
-
no activity observed with substrate peptides of other UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferases
-
-
?
additional information
?
-
no activity with 2'-deoxy-alpha-D-galactosamine or 2'-oxymethyl-alpha-D-galactosamine
-
-
?
additional information
?
-
-
no activity with 2'-deoxy-alpha-D-galactosamine or 2'-oxymethyl-alpha-D-galactosamine
-
-
?
additional information
?
-
-
enzyme structure, GT1 motif, DXD motif, Gal/GalNAc-T motif
-
-
?
additional information
?
-
-
UDP-GalNAc polypeptide:N-acetylgalactosaminyltransferase catalyzes the first step in the mucin-type O-glycan biosynthesis pathway by transferring GalNAc to Ser or Thr residues in a protein from the sugar donor UDP-GalNAc
-
-
?
additional information
?
-
-
different substrate specificities before and after Muc2 affinity chromatography of enzyme
-
-
?
additional information
?
-
-
not: lacto-N-fucopentaose I, ceramide dihexosides, ceramide trihexosides, globoside
-
-
?
additional information
?
-
-
contains a lectin-like repeat sequence at the C-terminus, role of GalNAc-T1 lectin domain, which is involved in glycosylation of substrates with multiple acceptor sites
-
-
?
additional information
?
-
ppGaNTase-T6: no activity with unglycosylated peptides
-
-
?
additional information
?
-
9 isoforms, ppGaNTase-T9 contains a 480-amino acid conserved region and shows no activity with non-glycosylated peptides
-
-
?
additional information
?
-
-
existence of multiple enzyme activities, differentially expressed in different organs, at least 2 distinct enzyme specificities
-
-
?
additional information
?
-
-
different substrate specificities before and after Muc2 affinity chromatography of enzyme
-
-
?
additional information
?
-
-
not: UDP-glucuronate, UDP-xylose, GDP-mannose, GDP-fucose, intact mucins, desialylated mucins, glycolipid acceptors, including globoside and ceramide hexosides, no transfer of GalNAc to terminal sugar residues in oligosaccharide chains
-
-
?
