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2.4.1.5: dextransucrase

This is an abbreviated version!
For detailed information about dextransucrase, go to the full flat file.

Word Map on EC 2.4.1.5

Reaction

sucrose
+
[(1->6)-alpha-D-glucosyl]n
=
D-fructose
+
[(1->6)-alpha-D-glucosyl]n+1

Synonyms

B-512F dextransucrase, B-512FMC dextransucrase, Cab3, CEP, DexT, dextran-sucrase, DS, DSase, DSR, Dsr S protein, DSR-F, DSR-S, DSRB742, DSRBCB4, DSRC39-2, DsrE563, DsrP, DSRS, DSRWC, DsrX, FT045B dextransucrase, glucansucrase, glucosyltransferase, glucosyltransferase, sucrose-1,6-alpha-glucan, glycosyltransferase R, Gtf, Gtf-DSM, GTFR, More, SGE, sucrose 6-glucosyltransferase, sucrose:1, 6-alpha-D-glucan 6-alpha-glucosyltransferase, sucrose:1,6-alpha-D-glucan-6-alpha-D-glucosyltransferase, Wc392-rDSR, WcCab3-DSR

ECTree

     2 Transferases
         2.4 Glycosyltransferases
             2.4.1 Hexosyltransferases
                2.4.1.5 dextransucrase

Specific Activity

Specific Activity on EC 2.4.1.5 - dextransucrase

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.012
-
purified recombinant mutant DRN4
0.027
-
purified recombinant mutant DRN2
0.037
-
purified recombinant wild-type DSRB742
0.185
-
purified recombinant mutant DRN3
0.287
-
purified recombinant mutant DRN1
0.4
-
commercial preparation, pH 5.2, 30°C
0.6
-
crude enzyme, culture supernatant, pH 5.4, 30°C
1
partially purrified recombinant enzyme, pH 5.4, 35°C
1.2
purified recombinant chimeric DSXR, dextransucrase activity, pH 5.8, 28°C
1.5
partially purrified recombinant enzyme, pH 5.0, 35°C
10.83
30°C, purified enzyme, at start of incubation, the reaction medium has pH 7.0
10.9
purified enzyme, pH 5.4, 35°C
126
purified recombinant enzyme
13.9
-
purified recombinant wild-type DSRBCB4
141
-
155000 Da enzyme form
145
-
170000 Da precursor
173.2
-
partially purified mutant enzyme, pH 5.0, 35°C
18
-
PEG 400-purified enzyme, pH 5.4, 30°C
18.9
recombinant His-tagged enzme, by and Nelson-Somogyi assay, pH 5.4, 35°C
20.83
30°C, purified enzyme, after 108 h of incubation, the reaction medium shows a pH of 4.0 from initially pH 7.0
23
-
purified enzyme, pH 5.4, 30°C
23.8
-
enzyme purified by the phase-partition using PEG 400
282
-
enzyme immobilized on carrageenan, pH and temperature not specified in the publication
31.3
-
partially purified wild-type enzyme, pH 4.5, 30°C
335.1
-
pH 5, 30°C
35.3
-
purified native enzyme
36
-
PEG 1500-purified enzyme, pH 5.4, 30°C
393
-
enzyme immobilized on calcium alginate, pH and temperature not specified in the publication
42.1
-
enzyme purified by the phase-partition using PEG 600
46
JX679020
purified native enzyme, pH 5.4, 30°C
54
-
cellobio-oligosaccharides synthesis
5830
-
pH 5.5, 37°C, enzyme immobilized on calcium-alginate beads. Substrate: sucrose
6062
-
pH 5.5, 37°C, soluble enzyme. Substrate: sucrose
61.7
-
enzyme immobilized on agar, pH and temperature not specified in the publication
686
purified recombinant enzyme, in presence of 0.1 mM Ca2+
7.2
recombinant His-tagged enzme, by the 14C-sucrose radioisotope assay, pH 5.4, 35°C
73.31
30°C, purified enzyme, after 60 h of incubation, the reaction medium shows a pH of 5.5 from initially pH 7.0
97.37
-
recombinant enzyme in crude enzyme extract
additional information